Pharmacognostic Evaluation and Antioxidant Activities of Tetracera indica (Christm. and Panz.) Merr

Vera Ladeska, Vera (2024) Pharmacognostic Evaluation and Antioxidant Activities of Tetracera indica (Christm. and Panz.) Merr. HAYATI J Biosci, 31 (5). pp. 836-853. ISSN Vol. 31 No. 5, September 2024

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Abstract

The community uses Tetracera indica (Christm. and Panz.) Merr extensively
as a traditional medicine. This plant was the main source of raw materials for
herbal medicinal products, so identification was needed to ensure its authenticity
through pharmacognosy evaluation. The present study evaluated macros- and
microscopic characteristics, performed phytochemical analysis, and performed
total phenolic (TPC) and flavonoid content (TFC) analysis. The organoleptic and
macroscopic studies were carried out through sensory organs. The microscopic
characteristics of leaf powder and twigs identification using chloral hydrate and
distilled water. Numerous chemical reagents, TLC methods and LC-MS/MS
analysis were employed in the phytochemical study. Research employed DPPH
and FRAP tests to assess the antioxidant activity. Furthermore, quercetin was
used as a marker of antioxidant activity. The morphological marker of the leaf
T. indica showed the surface of the leaves is rough bristles, shape elliptic-oblong,
margin serratus and phyllotaxy alternate. The stem is lignosus, and the rough
stem surface is slightly peeling. The lower leaf surface had paracytic stomata and
uniseriate trichomes with tapering ends. Raphide crystals and cork cells were
detected in the powder analysis of the twigs. The ethyl acetate extract's twig had
the highest TPC and TFC values. Ethyl acetate extract from Tetracera indica
twigs has potent antioxidant activity with IC50 = 99.84±0.011 μg/ml (DPPH) and
4296.67±0.024 mol/g (FRAP). The study showed that Tetracera indica contains
possible active components that could be developed as novel antioxidants. The
result of this study might provide early verification and identification to obtain
quality medicinal raw materials.

Item Type:	Article
Subjects:	Z Bibliography. Library Science. Information Resources > Z719 Libraries (General)
Depositing User:	Vera Ladeska
Date Deposited:	21 Jul 2025 01:13
Last Modified:	21 Jul 2025 01:13
URI:	http://repository.uhamka.ac.id/id/eprint/45085

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