An Investigation of Atorvastatin Calcium Loaded Transethosomes for Transdermal Delivery and Validation of HPLC Methods

Lestari, Pramulani Mulya (2024) An Investigation of Atorvastatin Calcium Loaded Transethosomes for Transdermal Delivery and Validation of HPLC Methods. Journal of Medicinal and Chemical Sciences, 7 (7). pp. 969-982. ISSN 2651-4702

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Abstract

Atorvastatin calcium is an antihyperlipidemic agent with low
bioavailability due to the first-pass metabolism after oral administration.
To overcome the limitation, an alternative method for delivering drug
molecules to the systemic circulation is needed, such as transethosomes
produced for transdermal delivery. Therefore, this study aimed to produce
atorvastatin calcium transethosomes with characteristics suitable for
transdermal delivery using validated analytical method. Transethosomes
formulations were prepared with different concentrations of soy lecithin
and ethanol. All formulations were evaluated for particle size, zeta
potential, polydispersity index (PDI), drug content, and deformability
index. Validation of the analytical method of atorvastatin calcium was
carried out using system suitability, selectivity, linearity, limit of detection
(LOD), limit of quantification (LOQ), precision, accuracy, and robustness.
The result showed that transethosomes had a small particle size of 123.90
nm-165.10 nm, high zeta potential values, acceptable PDI, the highest
atorvastatin calcium content, and good deformability index. In addition,
the system suitability test results were acceptable and there was no
interference in the atorvastatin calcium area. Linearity showed r =0.9999
with LOD and LOQ values of 0.388 µg/mL and 1.176 µg/mL, respectively.
The % RSD in the precision was < 2 and the recovery in the accuracy was
98.76%-101.04%. The method proved to be robust with respect to mobile
phase pH changes. Based on the results, atorvastatin calcium-loaded
transethosomes were suitable for transdermal delivery and could be
effectively produced with a 3% soy lecithin and 30% ethanol
concentration. The analytical method for determining drug content of
atorvastatin calcium-loaded transethosomes could be effectively applied.

Item Type:	Article
Subjects:	R Medicine
Divisions:	Fakultas Farmasi dan Sains > Farmasi
Depositing User:	Pramulani Mulya Lestari
Date Deposited:	21 Jul 2025 01:15
Last Modified:	21 Jul 2025 01:15
URI:	http://repository.uhamka.ac.id/id/eprint/45045

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