Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell line: A model system to understand Type 2 Diabetes Mellitus

Sri Suciati Ningsih, Suci dan Rizkyana Avissa, Kiki dan Endin Nokik Stujanna, Endin dan Erlin Listyaningsih, Erlin dan Takashi Yashiro, Yashiro dan Wawang Setiawan Sukarya, Wawang (2021) Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell line: A model system to understand Type 2 Diabetes Mellitus. Journal of Experimental and Clinical Medicine, 38 (3). pp. 211-215.

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Abstract

Type 2 Diabetes Mellitus (T2DM) is one of the major health issues in the world. The cellular mechanism of T2DM is still not fully understood.
It could be studied by using spheroid three-dimensional (3D) culture which is considered representative of the in vivo conditions. Several types
of pancreatic β cell lines have been used, one of which is the insulin-GLase (iGL) cell line. This study aims to evaluate the effect of cell density
and incubation time on spheroid morphology and cell viability in order to understand which one can be considered as the best option in studying
T2DM using iGL cell. Spheroid was made by using the Hanging drop method. The variations of initial seeding cells were 50, 100, 200, and 400
cells/µL then incubated for 1, 2, 3, and 4 days. The evaluated parameters in this study are spheroid morphology and cell viability. Spheroid
morphology was observed by using inverted phase contrast microscope integrated with camera (Nikon) and NIS-Elements Analysis D software.
Cell viability was determined by using LUNA-II™ Automated Cell Counter (Logos Biosystem). The result of this study showed that spheroid in
all of the group cell concentration have formed since the first day and its diameter was significantly increased on the following days (p<0,05).
The spheroid size was positively correlated with the cell density in group 50-200 cells/µL. A single and stable spheroid morphology was
observed in 50-100 cells/µL group. Cell viability in 3D culture system was lower and significantly decreased since day 3 compared to 2D culture
(p <0.05; 0.01). In conclusion, spheroid derived from iGL cell line with a stable morphology and good viability could be obtained from a cell
concentration of 50-100 cells / µL with two days of incubation.
Keywords: iGL, cell density, spheroid, viability

Item Type:	Article
Subjects:	R Medicine
Depositing User:	Endin Nokik Stujanna
Date Deposited:	08 Dec 2022 23:29
Last Modified:	29 Feb 2024 09:38
URI:	http://repository.uhamka.ac.id/id/eprint/17282

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