Optimization and Validation Methods for Assay of Acyclovir Cream Determination by High Performance Liquid Chromatography

Supandi Supandi, Supandi (2021) Optimization and Validation Methods for Assay of Acyclovir Cream Determination by High Performance Liquid Chromatography. In: 2nd International Conference Earth Science And Energy, 21-22 Oktober 2020, online.

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Abstract

The aim this study is to obtain an effective and efficient of method for
assay of acyclovir cream determination. Chromatographic separation on a reverse
phase a YMC - Triart C18 column (4.6 x 150 mm, 5 μm), the mobile phase
consisted of water and acetonitrile (95: 5 v/v) at a flow rate 1.2 mL/min with UV�Vis detection at 252 nm, volume injected was 20 μL, with UV detection at 252.0
nm. The retention time of acyclovir was ± 3.5 min. Validation studies were
achieved by using the limit of detection (LOD) and limit of quantification (LOQ),
precision, accuracy, selectivity parameter. The LOD and LOQ values were 0.27
µg/mL and 0.83 µg/mL. The average standard deviation (RSD) value of precision
is 0.35% and accuracy is 0.34%. Robustness shows that the method is resistant to
variation in the mobile phase, the Acyclovir cream analysis method meets the ICH
1994 requirements, so it can be applied to the assay of Acyclovir determination in
Cream dosage forms.

Item Type:	Conference or Workshop Item (Paper)
Additional Information:	-
Uncontrolled Keywords:	analysis
Divisions:	Fakultas Farmasi dan Sains > Farmasi
Depositing User:	Supandi Supandi
Date Deposited:	19 Aug 2021 14:09
Last Modified:	19 Aug 2021 14:09
URI:	http://repository.uhamka.ac.id/id/eprint/9197

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