The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.)

Wahyu Hidayati, Wahyu dan Ade Nur Padillah, Ade dan Maharadingga, Maharadingga dan Ni Putu Ermi Hikmawati, Ermi dan Rini Prastiwi, Rini dan Ani Pratiwi, Ani dan Lady Farahmayuni, Lady dan Rezza Syahputra, Rezza dan Muhammad Fahrul, Fahrul The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.). The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.).

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Abstract

Indonesian people use bay leaves as spices in local culinary and as traditional medicine, particularly to treat
diabetes. The problems with the mass production of antidiabetic drugs from bay leaves can be solved by
utilizing endophytic fungi as an alternative source for antidiabetic compounds. This study aimed to isolate
endophytic fungi from bay leaves and identify their antidiabetic activity through the in vitro inhibition of
alpha-amylase. The leaves were processed on potato dextrose agar media, and five isolates were grown in
an agar medium. The fermentation used a potato dextrose yeast medium that was left for five days on an
orbital shaker at room temperature. The crude was extracted using ethyl acetate solvent. In the in vitro
alpha-amylase inhibition test, the antidiabetic assay used the ethyl acetate extract of the endophytic fungi.
The inhibition percentage was calculated from the absorbance value read by a microplate reader. All isolates
inhibited alpha-amylase activity, but only three of them had high inhibition percentages (14.385%,
12.849%, and 39.246%). As a conclusion, the endophytic fungi isolated from bay leaves are potential as an
alternative source for the production of secondary metabolites to cure diabetes.

Item Type:	Article
Subjects:	R Medicine > R Medicine (General) R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Keguruan & Ilmu Pendidikan > Pendidikan Ekonomi
Depositing User:	Furqon FFS
Date Deposited:	04 Feb 2020 09:15
Last Modified:	04 Feb 2020 09:15
URI:	http://repository.uhamka.ac.id/id/eprint/871

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