Acute oral toxicity of sunda porcupine’s (Hystrix javanica, F. Cuvier 1823) quills crude extract on male sprague Dawley rats using fixed dose method

Budiman, Muhamad Arif Acute oral toxicity of sunda porcupine’s (Hystrix javanica, F. Cuvier 1823) quills crude extract on male sprague Dawley rats using fixed dose method. AIP Conf. Proc. 2972.

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Sunda porcupine (Hystrix javanica F. Cuvier 1823) is an endemic mammalian fauna from Indonesia known as
a pest since they damage crops like corn and tubers. Local people hunt the porcupine to decrease the damage, but others
hunt for its medicinal benefits. They believe that sunda porcupine has medicinal benefits, yet this ethnomedicine has not
been recorded well and has no sufficient scientific report. One of the interesting ethnomedicines is that local people
believe the quills of sunda porcupine can treat toothache. Our research aims to add a scientific record of the sunda
porcupine’s quills as well the acute toxicity effect of sunda porcupine’s quills crude extract on male Sprague Dawley rats.
The extraction of the active compound of the quills was using ethanol 70%. The determination of acute toxicity effect
was using a fixed dose method by oral administration, including two steps: sighting study and main study, with four
doses: 5 mg/kg; 50 mg/kg; 300 mg/kg; and 2.000 mg/kg. We also observed the histological effect on liver and kidney
tissues. The result showed that the oral administration of the sunda porcupine’s quills crude extract by the method of the
fixed dose does not cause death in all rats used both after 24 hours (acute effect) and 14 days (delayed effect). Based on
the fixed dose method, we can categorize the sunda porcupine’s quills crude extract as non-toxic. Although the rats were
clinically healthy and showed no symptoms of intoxication during 14 days of observation, histopathological analysis of
the liver and kidney showed lesions which cannot be ignored. Histopathological analysis showed that the higher the dose,
the lesions tended to increase the severity on the liver and kidney, but the active substance administered did not kill the
rats and the severity was mostly mild. It is in line with the percentage of body weight gain, which is a group of placebo >
300 mg/kg > 2000 mg/kg, which may occur because the body prioritizes repairing the damage of the tissues due to the
exact administration rather than for growth. These findings can be used as consideration for further toxicity study on
sunda porcupine’s quills crude extract, such as chronic and subchronic toxicity tests, both to ensure the safety and to
complete the toxicity study of the extract.

Item Type: Article
Subjects: Q Science > QD Chemistry
Q Science > QL Zoology
R Medicine > R Medicine (General)
Divisions: Fakultas Kedokteran > Kedokteran
Depositing User: Muhamad Arif Budiman
Date Deposited: 31 Jan 2024 07:16
Last Modified: 31 Jan 2024 07:16

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