eprintid: 9197
rev_number: 10
eprint_status: archive
userid: 1558
dir: disk0/00/00/91/97
datestamp: 2021-08-19 14:09:42
lastmod: 2021-08-19 14:09:42
status_changed: 2021-08-19 14:09:42
type: conference_item
metadata_visibility: show
creators_name: Supandi Supandi, Supandi
creators_id: supandi@uhamka.ac.id
contributors_type: http://www.loc.gov/loc.terms/relators/AUT
contributors_name: Supandi, Supandi
contributors_id: supandi@uhamka.ac.i
corp_creators: UHAMKA
title: Optimization and Validation Methods for Assay of Acyclovir 
Cream Determination by High Performance Liquid 
Chromatography
ispublished: pub
divisions: 48201
keywords: analysis
note: -
abstract: The aim this study is to obtain an effective and efficient of method for 
assay of acyclovir cream determination. Chromatographic separation on a reverse 
phase a YMC - Triart C18 column (4.6 x 150 mm, 5 μm), the mobile phase 
consisted of water and acetonitrile (95: 5 v/v) at a flow rate 1.2 mL/min with UV�Vis detection at 252 nm, volume injected was 20 μL, with UV detection at 252.0 
nm. The retention time of acyclovir was ± 3.5 min. Validation studies were 
achieved by using the limit of detection (LOD) and limit of quantification (LOQ), 
precision, accuracy, selectivity parameter. The LOD and LOQ values were 0.27 
µg/mL and 0.83 µg/mL. The average standard deviation (RSD) value of precision 
is 0.35% and accuracy is 0.34%. Robustness shows that the method is resistant to 
variation in the mobile phase, the Acyclovir cream analysis method meets the ICH 
1994 requirements, so it can be applied to the assay of Acyclovir determination in 
Cream dosage forms.
date: 2021-07-04
date_type: published
official_url: https://iopscience.iop.org/article/10.1088/1755-1315/819/1/012055/pdf
full_text_status: public
pres_type: paper
pagerange: 1-8
event_title: 2nd International Conference Earth Science And Energy
event_location: online
event_dates: 21-22 Oktober 2020
event_type: conference
related_url_url: https://iopscience.iop.org/article
related_url_type: pub
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[3] Departemen Kesehatan RI, “UU RI No 36 Tentang Kesehatan,” 2009. 
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Farmakope Indonesia Edisi V, Edisi V., Jakarta: Departemen Kesehatan, 2014, pp. 173–176. 
[5] P. D. Tzanavaras and C. K. Zacharis, “Extraction of acyclovir from pharmaceutical creams for 
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[6] Harmita H., “Petunjuk Pelaksanaan Validasi Metode Dan Cara Perhitungannya,” Majalah Ilmu 
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funders: -
projects: -
citation:   Supandi Supandi, Supandi  (2021) Optimization and Validation Methods for Assay of Acyclovir Cream Determination by High Performance Liquid Chromatography.  In: 2nd International Conference Earth Science And Energy, 21-22 Oktober 2020, online.     
document_url: http://repository.uhamka.ac.id/id/eprint/9197/1/IOP_1.pdf