TY  - CONF
Y1  - 2021/07/04/
A1  - Supandi Supandi, Supandi
M2  - online
N2  - The aim this study is to obtain an effective and efficient of method for 
assay of acyclovir cream determination. Chromatographic separation on a reverse 
phase a YMC - Triart C18 column (4.6 x 150 mm, 5 ?m), the mobile phase 
consisted of water and acetonitrile (95: 5 v/v) at a flow rate 1.2 mL/min with UV?Vis detection at 252 nm, volume injected was 20 ?L, with UV detection at 252.0 
nm. The retention time of acyclovir was ± 3.5 min. Validation studies were 
achieved by using the limit of detection (LOD) and limit of quantification (LOQ), 
precision, accuracy, selectivity parameter. The LOD and LOQ values were 0.27 
µg/mL and 0.83 µg/mL. The average standard deviation (RSD) value of precision 
is 0.35% and accuracy is 0.34%. Robustness shows that the method is resistant to 
variation in the mobile phase, the Acyclovir cream analysis method meets the ICH 
1994 requirements, so it can be applied to the assay of Acyclovir determination in 
Cream dosage forms.
KW  - analysis
ID  - repository9197
UR  - https://iopscience.iop.org/article/10.1088/1755-1315/819/1/012055/pdf
AV  - public
N1  - -
T2  - 2nd International Conference Earth Science And Energy
TI  - Optimization and Validation Methods for Assay of Acyclovir 
Cream Determination by High Performance Liquid 
Chromatography
SP  - 1
EP  - 8
ER  -