eprintid: 871
rev_number: 10
eprint_status: archive
userid: 1193
dir: disk0/00/00/08/71
datestamp: 2020-02-04 09:15:31
lastmod: 2020-02-04 09:15:31
status_changed: 2020-02-04 09:15:31
type: article
metadata_visibility: show
creators_name: Wahyu Hidayati, Wahyu
creators_name: Ade Nur Padillah, Ade
creators_name: Maharadingga, Maharadingga
creators_name: Ni Putu Ermi Hikmawati, Ermi
creators_name: Rini Prastiwi, Rini
creators_name: Ani Pratiwi, Ani
creators_name: Lady Farahmayuni, Lady
creators_name: Rezza Syahputra, Rezza
creators_name: Muhammad Fahrul, Fahrul
creators_id: wahyu_hidayati@uhamka.ac.id
creators_id: ermy0907@uhamka.ac.id
creators_id: rini_prastiwi@uhamka.ac.id
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
corp_creators: UHAMKA
corp_creators: UHAMKA
corp_creators: UHAMKA
corp_creators: UHAMKA
title: The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.)
ispublished: pub
subjects: R1
subjects: RS
divisions: sch_med
abstract: Indonesian people use bay leaves as spices in local culinary and as traditional medicine, particularly to treat
diabetes. The problems with the mass production of antidiabetic drugs from bay leaves can be solved by
utilizing endophytic fungi as an alternative source for antidiabetic compounds. This study aimed to isolate
endophytic fungi from bay leaves and identify their antidiabetic activity through the in vitro inhibition of
alpha-amylase. The leaves were processed on potato dextrose agar media, and five isolates were grown in
an agar medium. The fermentation used a potato dextrose yeast medium that was left for five days on an
orbital shaker at room temperature. The crude was extracted using ethyl acetate solvent. In the in vitro
alpha-amylase inhibition test, the antidiabetic assay used the ethyl acetate extract of the endophytic fungi.
The inhibition percentage was calculated from the absorbance value read by a microplate reader. All isolates
inhibited alpha-amylase activity, but only three of them had high inhibition percentages (14.385%,
12.849%, and 39.246%). As a conclusion, the endophytic fungi isolated from bay leaves are potential as an
alternative source for the production of secondary metabolites to cure diabetes.
publisher: Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development (MICH-PhD 2018)
full_text_status: public
publication: The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.)
refereed: TRUE
citation:   Wahyu Hidayati, Wahyu dan Ade Nur Padillah, Ade dan Maharadingga, Maharadingga dan Ni Putu Ermi Hikmawati, Ermi dan Rini Prastiwi, Rini dan Ani Pratiwi, Ani dan Lady Farahmayuni, Lady dan Rezza Syahputra, Rezza dan Muhammad Fahrul, Fahrul   The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.).  The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.).       
document_url: http://repository.uhamka.ac.id/id/eprint/871/1/MICH-PhD_2018_19.pdf