<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)"^^ . "Reconstructed Human Epidermis (RHE) is an artificial epidermis\r\nmade in such a way that it resembles human skin, and can be used for the\r\nidentification of irritant chemicals, especially for cosmetic and topical medicinal\r\nproducts. Currently the new RHE is produced by European and American\r\ncountries, whose skin physiology is very different from Indonesia. Based on\r\nthis, the Center for Research on Drugs and Food, NADFC of Republic of\r\nIndonesia took the initiative to develop the reconstruction of keratinocyte,\r\nmelanocytes and fibroblasts cells into RHE adapted to the anatomical and\r\nphysiological functions of the skin of Indonesians. RHE is made from an epidermal\r\nlayer composed of keratinocyte and melanocytes cells that are reconstructed\r\nwith a dermis layer composed of fibroblast and collagen cells.\r\nKeratinocyte, melanocytes and fibroblasts cells are cultured on suitable mediums\r\nby adding a suitable growth medium. To find out that RHE has been\r\nsuccessfully reconstructed, measured percentage of cell life, made histology\r\npreparation to see the existence of cell nucleus, and conducted Immunohistochemical\r\nexamination to see existence of integration (bond) between antigen.\r\nFrom the research results can be seen that keratinocyte cells grown on culture\r\nmedium Keratinocyte SFM (IX) with rEGF supplements; melanocyte cells\r\ngrown on Melanocyte 254 culture medium with HMGS supplementation; and\r\nfibroblast cells grown on Fibroblast M 106 culture medium with LSGS supplementation.\r\nThe percentage of epidermal cell life grew well in the planting of\r\n10 � 104 cells /mL keratinocytes and 0.25 � 104 cells /mL of melanocyte cells\r\nand survived until the 11th day with live cell percentage of 93.45%. In making\r\npreparation for histology with HE staining, there is a cell life in RHE tissue.\r\nUsed Immunohistochemical (IHC) examination using cytokeratin 10 antibody\r\nmarker to view physiological function of epidermal tissue."^^ . . . "doi.org"^^ . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)"^^ . . . . . . . . . . . . . . . . . . . . . . . "Murtiningsih"^^ . "Murtiningsih"^^ . "Murtiningsih Murtiningsih"^^ . . "Rina"^^ . "Rina Adriany"^^ . "Rina Rina Adriany"^^ . . "Tuty"^^ . "Tuty Erlina"^^ . "Tuty Tuty Erlina"^^ . . "Herlina"^^ . "Herlina B. Setijanti"^^ . "Herlina Herlina B. Setijanti"^^ . . "Rahmi"^^ . "Rahmi Fitria"^^ . "Rahmi Rahmi Fitria"^^ . . "Eka"^^ . "Eka Rusmawati"^^ . "Eka Eka Rusmawati"^^ . . "UHAMKA"^^ . . . . . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE) (Text)"^^ . . . "2019_Chapter_.pdf"^^ . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE) (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE) (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE) (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE) (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE) (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #822 \n\nDevelopment the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)\n\n" . "text/html" . . . "R Medicine (General)"@en . . . "RS Pharmacy and materia medica"@en . .