%0 Journal Article
%A Herlina B. Setijanti, Herlina
%A Eka Rusmawati, Eka
%A Rahmi Fitria, Rahmi
%A Tuty Erlina, Tuty
%A Rina Adriany, Rina
%A Murtiningsih, Murtiningsih
%A UHAMKA,
%F repository:822
%I doi.org
%J Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)
%T Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)
%U http://repository.uhamka.ac.id/id/eprint/822/
%X Reconstructed Human Epidermis (RHE) is an artificial epidermis  made in such a way that it resembles human skin, and can be used for the  identification of irritant chemicals, especially for cosmetic and topical medicinal  products. Currently the new RHE is produced by European and American  countries, whose skin physiology is very different from Indonesia. Based on  this, the Center for Research on Drugs and Food, NADFC of Republic of  Indonesia took the initiative to develop the reconstruction of keratinocyte,  melanocytes and fibroblasts cells into RHE adapted to the anatomical and  physiological functions of the skin of Indonesians. RHE is made from an epidermal  layer composed of keratinocyte and melanocytes cells that are reconstructed  with a dermis layer composed of fibroblast and collagen cells.  Keratinocyte, melanocytes and fibroblasts cells are cultured on suitable mediums  by adding a suitable growth medium. To find out that RHE has been  successfully reconstructed, measured percentage of cell life, made histology  preparation to see the existence of cell nucleus, and conducted Immunohistochemical  examination to see existence of integration (bond) between antigen.  From the research results can be seen that keratinocyte cells grown on culture  medium Keratinocyte SFM (IX) with rEGF supplements; melanocyte cells  grown on Melanocyte 254 culture medium with HMGS supplementation; and  fibroblast cells grown on Fibroblast M 106 culture medium with LSGS supplementation.  The percentage of epidermal cell life grew well in the planting of  10 � 104 cells /mL keratinocytes and 0.25 � 104 cells /mL of melanocyte cells  and survived until the 11th day with live cell percentage of 93.45%. In making  preparation for histology with HE staining, there is a cell life in RHE tissue.  Used Immunohistochemical (IHC) examination using cytokeratin 10 antibody  marker to view physiological function of epidermal tissue.