eprintid: 818
rev_number: 7
eprint_status: archive
userid: 1193
dir: disk0/00/00/08/18
datestamp: 2019-12-19 05:50:35
lastmod: 2019-12-19 05:50:35
status_changed: 2019-12-19 05:50:35
type: article
metadata_visibility: show
creators_name: Fitri Yuniarti, Fitri
creators_name: Wahyu Hidayati, Wahyu
creators_name: Lulu Shofaya, Lulu
corp_creators: UHAMKA
corp_creators: UHAMKA
title: Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)
ispublished: pub
subjects: R1
subjects: RS
divisions: sch_med
abstract: Soursop leaves (Annona muricata L.) is one of the medicinal plants identified as a source of endophytic
bacteria producing secondary metabolites. Several studies have reported that secondary metabolite
compounds extracted from soursop leaves have inhibitory activity against pathogenic bacteria and fungi and
have anticancer activity. This study aims to isolate endophytic bacteria of soursop leaf and identify
molecular isolates producing antibacterial metabolites by PCR method. This study began with endophytic
bacterial isolation of soursop leaves, followed by screening for antibacterial potency using disc diffusion
method and identification of molecular isolates which had the highest antibacterial activity. After isolation,
three isolates were obtained: BW-1LM, BW-2LP, and BW-3LK. The result of antibacterial activity test
showed that BW-1LM isolate had the highest activity against bacterium of Bacillus subtilis and Salmonella
typhi test. Molecular identification was obtained by BW-1LW isolate having 99% similarity level to
Bacillus licheniformis DSM 13 strain. The conclusion is soursop leaves contains endophytic bacteria which
have antibacterial activity against Bacillus subtilis and Salmonella typhi.
publisher: In Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development (MICH-PhD 2018)
full_text_status: public
publication: Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)
refereed: TRUE
citation:   Fitri Yuniarti, Fitri dan Wahyu Hidayati, Wahyu dan Lulu Shofaya, Lulu   Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.).  Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.).       
document_url: http://repository.uhamka.ac.id/id/eprint/818/1/MICH-PhD_2018_29.pdf