relation: http://repository.uhamka.ac.id/id/eprint/818/
title: Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)
creator: Fitri Yuniarti, Fitri
creator: Wahyu Hidayati, Wahyu
creator: Lulu Shofaya, Lulu
subject: R Medicine (General)
subject: RS Pharmacy and materia medica
description: Soursop leaves (Annona muricata L.) is one of the medicinal plants identified as a source of endophytic  bacteria producing secondary metabolites. Several studies have reported that secondary metabolite  compounds extracted from soursop leaves have inhibitory activity against pathogenic bacteria and fungi and  have anticancer activity. This study aims to isolate endophytic bacteria of soursop leaf and identify  molecular isolates producing antibacterial metabolites by PCR method. This study began with endophytic  bacterial isolation of soursop leaves, followed by screening for antibacterial potency using disc diffusion  method and identification of molecular isolates which had the highest antibacterial activity. After isolation,  three isolates were obtained: BW-1LM, BW-2LP, and BW-3LK. The result of antibacterial activity test  showed that BW-1LM isolate had the highest activity against bacterium of Bacillus subtilis and Salmonella  typhi test. Molecular identification was obtained by BW-1LW isolate having 99% similarity level to  Bacillus licheniformis DSM 13 strain. The conclusion is soursop leaves contains endophytic bacteria which  have antibacterial activity against Bacillus subtilis and Salmonella typhi.
publisher: In Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development (MICH-PhD 2018)
type: Article
type: PeerReviewed
format: text
language: en
identifier: http://repository.uhamka.ac.id/id/eprint/818/1/MICH-PhD_2018_29.pdf
identifier:   Fitri Yuniarti, Fitri dan Wahyu Hidayati, Wahyu dan Lulu Shofaya, Lulu   Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.).  Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.).