TY  - JOUR
SN  - 2477 0256
JF  - Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient
UR  - http://repository.uhamka.ac.id/id/eprint/812/
PB  - Pharmaciana
ID  - repository812
N2  - A therapy development with recombinant protein is a new and potential innovation to destroy
cancer stem cells (CSCs). Various ways of killing CSCs include the provision of polyvalent antiprotein
antibodies that code pluripotency. Therefore, it takes a mixture of Oct-4, c-Myc, Sox2, and
Klf4 protein antigens that can stimulate the formation of polyvalent antibodies. This study aimed to
construct Sox2 recombinant by identifying the target genes by reverse transcriptase PCR and then
arranging their designs to be inserted into the cloning vector pET101/D-TOPOŽ. The target gene was
developed by finding the complete sequences of Sox2 nucleotides on the NCBI GenBank. The growth
on LB-ampicillin agar plates was amplified by PCR to obtain colonies with pET101/D-TOPOŽ
vectors and inserts of the pluripotent gene of CSCs, then the PCR results were observed through
electrophoresis. A total of fifteen colonies have DNA bands with a base pair of about 300 bp in length.
The recombinant clones produced Sox2 genes with a base length of 330 bp.
TI  - Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient
A1  - Hanifah, Rahmi
A1  - Tiodinar Theresia, Tiodinar
A1  - Amarila Malik, Amarila
A1  - Septelia Inawati Wanandi, Septelia
AV  - public
ER  -