relation: http://repository.uhamka.ac.id/id/eprint/812/
title: Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient
creator: Hanifah, Rahmi
creator: Tiodinar Theresia, Tiodinar
creator: Amarila Malik, Amarila
creator: Septelia Inawati Wanandi, Septelia
subject: R Medicine (General)
subject: RS Pharmacy and materia medica
description: A therapy development with recombinant protein is a new and potential innovation to destroy  cancer stem cells (CSCs). Various ways of killing CSCs include the provision of polyvalent antiprotein  antibodies that code pluripotency. Therefore, it takes a mixture of Oct-4, c-Myc, Sox2, and  Klf4 protein antigens that can stimulate the formation of polyvalent antibodies. This study aimed to  construct Sox2 recombinant by identifying the target genes by reverse transcriptase PCR and then  arranging their designs to be inserted into the cloning vector pET101/D-TOPO®. The target gene was  developed by finding the complete sequences of Sox2 nucleotides on the NCBI GenBank. The growth  on LB-ampicillin agar plates was amplified by PCR to obtain colonies with pET101/D-TOPO®  vectors and inserts of the pluripotent gene of CSCs, then the PCR results were observed through  electrophoresis. A total of fifteen colonies have DNA bands with a base pair of about 300 bp in length.  The recombinant clones produced Sox2 genes with a base length of 330 bp.
publisher: Pharmaciana
type: Article
type: PeerReviewed
format: text
language: en
identifier: http://repository.uhamka.ac.id/id/eprint/812/1/11919-33934-1-PB.pdf
identifier:   Hanifah, Rahmi dan Tiodinar Theresia, Tiodinar dan Amarila Malik, Amarila dan Septelia Inawati Wanandi, Septelia   Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient.  Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient.   ISSN 2477 0256