eprintid: 753
rev_number: 11
eprint_status: archive
userid: 1193
dir: disk0/00/00/07/53
datestamp: 2019-12-18 04:57:14
lastmod: 2020-11-02 08:37:39
status_changed: 2019-12-18 04:57:14
type: article
metadata_visibility: show
creators_name: Prihartini Widiyanti, Prihartini
creators_name: Bambang Prajogo, Bambang
creators_name: Ni Putu Ermi Hikmawati, Ermi
creators_id: ermy0907@uhamka.ac.id
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
corp_creators: UHAMKA
title: CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF
EXTRACTS ON MOLT-4 CELL
ispublished: pub
subjects: H
divisions: 12
abstract: Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present
study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the
fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The
treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a
wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by
viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to
determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract
and 70% ethanol extract of J. gendarussa leaves were 94 μg/ml and 78 μg/ml, respectively. The cytotoxicity of fractionated-extract and
70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract
and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.
publisher: Indonesian Journal of Tropical and Infectious Disease
full_text_status: public
publication: CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL
refereed: TRUE
citation:   Prihartini Widiyanti, Prihartini dan Bambang Prajogo, Bambang dan Ni Putu Ermi Hikmawati, Ermi   CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL.  CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL.       
document_url: http://repository.uhamka.ac.id/id/eprint/753/1/CYTOTOXICITY_OF_JUSTICIA_GENDARUSSA_BURM_F_LEAF_EX.pdf