eprintid: 56429
rev_number: 8
eprint_status: archive
userid: 2314
dir: disk0/00/05/64/29
datestamp: 2026-09-16 09:23:19
lastmod: 2026-09-16 09:23:19
status_changed: 2026-09-16 09:23:19
type: thesis
metadata_visibility: show
creators_name: RARA NADIKA, RARA
title: SKRIPSI-2210015037-RARA NADIKA-REPOSITORY
ispublished: pub
subjects: R
subjects: Skripsi
divisions: 11201
abstract: Obesitas merupakan kondisi patologis yang ditandai oleh akumulasi jaringan adiposa berlebih
dan dikaitkan dengan peningkatan produksi reactive oxygen species (ROS) yang
memicu stres oksidatif dan penurunan kadar glutathione (GSH). Holothuria atra merupakan
spesies teripang yang mengandung senyawa bioaktif yang diduga mampu memodulasi
kapasitas antioksidan endogen. Penelitian ini bertujuan untuk mengevaluasi efek pemberian
ekstrak H. atra terhadap kadar GSH aorta pada tikus obesitas yang diinduksi high
fat diet (HFD). Penelitian eksperimental in vivo ini menggunakan tikus jantan Sprague
Dawley yang dibagi menjadi tiga kelompok: kelompok Pakan Normal, kelompok Pakan
HFD, dan kelompok Pakan HFD+Ekstrak H. atra dengan dosis 300 mg/kgBB selama 6
minggu. Kadar GSH aorta diukur menggunakan metode ELISA. Hasil penelitian menunjukkan
bahwa kadar GSH aorta kelompok Pakan HFD+Ekstrak H. atra secara signifikan
lebih tinggi dibandingkan kelompok Pakan HFD (3,36 ± 0,15 μg/mL vs 1,70 ± 0,02 μg/mL;
p < 0,001), meskipun belum mencapai kadar kelompok Pakan Normal (10,38 ± 1,08
μg/mL). Temuan ini mengindikasikan bahwa ekstrak H. atra berpotensi meningkatkan kapasitas
antioksidan endogen pada jaringan aorta tikus obesitas.
Kata kunci: glutathione, high fat diet, Holothuria atra, obesitas, Sprague Dawley
date: 2026
date_type: published
full_text_status: restricted
institution: Universitas Muhammadiyah Prof. DR. Hamka
department: PROGRAM STUDI PENDIDIKAN DOKTER
thesis_type: bachelor
thesis_name: bphil
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citation:   RARA NADIKA, RARA  (2026) SKRIPSI-2210015037-RARA NADIKA-REPOSITORY.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. Hamka.   
document_url: http://repository.uhamka.ac.id/id/eprint/56429/1/SKRIPSI-2210015037-RARA%20NADIKA-REPOSITORY.pdf