eprintid: 56382
rev_number: 8
eprint_status: archive
userid: 2314
dir: disk0/00/05/63/82
datestamp: 2026-09-14 09:02:13
lastmod: 2026-09-14 09:02:13
status_changed: 2026-09-14 09:02:13
type: thesis
metadata_visibility: show
creators_name: FATIH ABDURRAHMAN A, FATIH
title: SKRIPSI-2210015031-FATIH ABDURRAHMAN A-REPOSITORY
ispublished: pub
subjects: R
subjects: Skripsi
divisions: 11201
abstract: Eksplorasi agen sitotoksik bahan alam diperlukan untuk mengatasi efek samping dari terapi
konvensional yang sudah ada pada kanker payudara subtipe Luminal A. Penelitian in vitro
ini bertujuan mengevaluasi efek ekstrak metanol daun Mangifera sumbawaensis terhadap
viabilitas dan morfologi sel kanker payudara MCF-7. Komponen bioaktif diekstraksi
melalui maserasi metanol, lalu diidentifikasi menggunakan Gas Chromatography-Mass
Spectrometry (GC-MS). Uji sitotoksik selanjutnya dilakukan via MTT assay pada
konsentrasi 62,5–1000 ppm dengan inkubasi selama 24 jam. Analisis data menggunakan
One-Way ANOVA dan regresi linear untuk menentukan nilai IC!". Hasil GC-MS
menunjukkan profil metabolit didominasi ester asam lemak dengan Methyl cis-13-
octadecenoate (20,37%) sebagai komponen utama. Secara mikroskopis, paparan ekstrak
memicu perubahan morfologi berupa sel membulat, pengerutan sitoplasma, dan serpihan
apoptosis pada konsentrasi 500 dan 1000 ppm. Walaupun perlakuan ekstrak Mangifera
pada konsentrasi 1000 ppm mampu menurunkan viabilitas sel hingga 47,15%, tren
penurunan tersebut secara statistik tidak menunjukkan perbedaan yang signifikan (p =
0,1044). Perhitungan regresi linear menunjukkan estimasi nilai IC!" sebesar 947,152 ppm.
Hal ini menunjukkan bahwa ekstrak metanol daun Mangifera sumbawaensis memiliki
aktivitas sitotoksik yang lemah, namun tetap berpotensi memicu apoptosis pada konsentrasi
tinggi.
Kata Kunci: Mangifera sumbawaensis, MCF-7, MTT assay, Sitotoksisitas Lemah.
date: 2026
date_type: published
full_text_status: restricted
institution: Universitas Muhammadiyah Prof. DR. Hamka
department: PROGRAM STUDI PENDIDIKAN DOKTER
thesis_type: bachelor
thesis_name: bphil
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citation:   FATIH ABDURRAHMAN A, FATIH  (2026) SKRIPSI-2210015031-FATIH ABDURRAHMAN A-REPOSITORY.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. Hamka.   
document_url: http://repository.uhamka.ac.id/id/eprint/56382/1/SKRIPSI-2210015031-FATIH%20ABDURRAHMAN%20A-REPOSITORY.pdf