eprintid: 56377
rev_number: 8
eprint_status: archive
userid: 2314
dir: disk0/00/05/63/77
datestamp: 2026-09-14 08:06:23
lastmod: 2026-09-14 08:06:23
status_changed: 2026-09-14 08:06:23
type: thesis
metadata_visibility: show
creators_name: SALZABILLA SYAHRAJAB, SALZABILLA
title: SKRIPSI-2210015026-SALZABILLA SYAHRAJAB-REPOSITORY
ispublished: pub
subjects: R
subjects: Skripsi
divisions: 11201
abstract: Penelitian ini bertujuan untuk menentukan efek sitotoksik ekstrak metanol daun Rasamala
Altingia excelsa Noronha terhadap viabilitas sel kanker payudara MCF-7 secara in vitro.
Penelitian ini menggunakan metode penelitian eksperimental, yang kemudian terbagi
menjadi kelompok kontrol serta kelompok perlakuan dengan variasi konsentrasi ekstrak
39,06; 78,125; 156,25; dan 312,5 μg/mL. Karakterisasi senyawa aktif fitokimia dianalisis
menggunakan instrumen Gas Chromatography-Mass Spectrometry (GC-MS) yang
memperlihatkan dominasi senyawa golongan terpenoid dan ester asam lemak. Efek
perlakuan dievaluasi berdasarkan pengamatan kualitatif perubahan morfologi sel
menggunakan mikroskop inversi yang memperlihatkan adanya tanda kerusakan sel akibat
paparan ekstrak berupa gejala pengerutan (cell shrinkage), perubahan bentuk menjadi
membulat (cell rounding), serta akumulasi debris sel seiring peningkatan konsentrasi
perlakuan. Uji viabilitas kuantitatif dilakukan dengan metode MTT assay dan di lakukan
analisis data menggunakan uji One-Way ANOVA dan analisis regresi linear untuk
menentukan nilai Inhibitory Concentration 50 (IC50). Hasil penelitian menunjukkan bahwa
ekstrak metanol daun Rasamala mampu menurunkan viabilitas sel MCF-7 secara signifikan
seiring peningkatan konsentrasi perlakuan (dose-dependent manner) dengan p < 0,0001.
Nilai IC50 ekstrak metanol daun Rasamala terhadap sel MCF-7 diperoleh sebesar 191,67
μg/mL yang menunjukkan aktivitas sitotoksik kategori sedang (moderate cytotoxic
activity).
Kata kunci: Altingia excelsa Noronha, aktivitas sitotoksik, IC50, MCF-7, MTT assay.
date: 2026
date_type: published
full_text_status: restricted
institution: Universitas Muhammadiyah Prof. DR. Hamka
department: PROGRAM STUDI PENDIDIKAN DOKTER
thesis_type: bachelor
thesis_name: bphil
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citation:   SALZABILLA SYAHRAJAB, SALZABILLA  (2026) SKRIPSI-2210015026-SALZABILLA SYAHRAJAB-REPOSITORY.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. Hamka.   
document_url: http://repository.uhamka.ac.id/id/eprint/56377/1/SKRIPSI-2210015026-SALZABILLA%20SYAHRAJAB-REPOSITORY.pdf