eprintid: 56373
rev_number: 8
eprint_status: archive
userid: 2314
dir: disk0/00/05/63/73
datestamp: 2026-09-14 07:22:26
lastmod: 2026-09-14 07:22:26
status_changed: 2026-09-14 07:22:26
type: thesis
metadata_visibility: show
creators_name: CUT ASHILAH HAURA, ASHILAH
title: SKRIPSI-2210015020-CUT ASHILAH HAURA-REPOSITORY
ispublished: pub
subjects: R
subjects: Skripsi
divisions: 11201
abstract: Resistensi antibiotik yang terus meningkat mendorong pencarian sumber
antibakteri alternatif dari bahan alam. Bellis parennis (bunga daisy) diketahui
mengandung berbagai senyawa bioaktif yang berpotensi sebagai antibakteri.
Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak Bellis parennis
terhadap pertumbuhan Staphylococcus aureus dan Escherichia coli secara in vitro
serta menentukan konsentrasi yang memberikan aktivitas antibakteri terbaik.
Penelitian ini merupakan true experimental laboratory research dengan rancangan
posttest-only control group design. Ekstrak Bellis parennis diperoleh melalui
metode maserasi menggunakan etanol 96% dan diuji pada konsentrasi 0,67 g/mL,
1 g/mL dan 3 g/mL. Aktivitas antibakteri dievaluasi menggunakan metode difusi
cakram Kirby–Bauer terhadap Staphylococcus aureus ATCC 25923 dan
Escherichia coli ATCC 25922, dengan ciprofloxacin sebagai kontrol positif dan
DMSO sebagai kontrol negatif. Data dianalisis menggunakan uji Kruskal–Wallis
dan Mann-Whitney. Hasil penelitian menunjukkan bahwa ekstrak Bellis parennis
memiliki aktivitas antibakteri terhadap kedua baktersi uji. Pada Staphylococcus
aureus, zona hambat terbentuk pada seluruh konsentrasi yang diuji, sedangkan pada
Escherichia coli zona hambat hanya terbentuk pada konsentrasi 3 g/mL. Uji
Kruskal-Wallis menunjukkan tidak terdapat perbedaan bermakna antar konsentrasi
pada Staphylococcus aureus (p=0,060), tetapi terhadap perbedaan bermakna pada
Escherichia coli (p=0,021). Uji Mann-Whitney menunjukkan bermakna antara
Staphylococcus aureus dan Escherichia coli pada seluruh konsentrasi (p<0,05).
Disimpulkan bahwa ekstrak Bellis parennis memiliki aktivitas antibakteri secara in
vitro dan lebih efektif terhadap Staphylococcus aureus dibandingkan Escherichia
coli.
Kata kunci: Bellis parennis, antibakteri, Staphylococcus aureus, Escherichia
coli, difusi cakram Kirby–Bauer.
date: 2026
date_type: published
full_text_status: restricted
institution: Universitas Muhammadiyah Prof. DR. Hamka
department: PROGRAM STUDI PENDIDIKAN DOKTER
thesis_type: bachelor
thesis_name: bphil
referencetext: Abdallah, E. M., Alhatlani, B. Y., de Paula Menezes, R., & Martins, C. H. G.
(2023). Back to Nature: Medicinal Plants as Promising Sources for
Antibacterial Drugs in the Post-Antibiotic Era. Plants (Basel, Switzerland),
12(17). https://doi.org/10.3390/plants12173077
Ahmed Bahram Wlia, & Soma Majedi. (2025). Optimizing hygiene and microbial
aspect of paper recycling: a sustainable approach for environmental
conservation. Cellular and Molecular Biology, 71(3), 151–157.
https://doi.org/10.14715/cmb/2025.71.3.18
Albien, A.-L., & Stark, T. D. (2023). (Bio)active Compounds in Daisy Flower
(Bellis perennis). Molecules (Basel, Switzerland), 28(23). https://doi.org/10.33
90/molecules28237716
Al-Saleh, A., Shahid, M., Farid, E., & Bindayna, K. (2022). Trends in methicillinresistant
Staphylococcus aureus in the Gulf Cooperation Council countries:
antibiotic resistance, virulence factors and emerging strains. Eastern
Mediterranean Health Journal, 28(6), 434443. https://doi.org/10.26719/emhj.2
2.042
Anggraini, L., Wahyu Margi Sidoretno, & Liza Salsabila Rifwan Putri. (2022). Uji
Efektivitas Antibakteri Ekstrak Metanol Daun Bandotan (Ageratum
conyzoides L.) Terhadap Staphylococcus Aureus. SEHATMAS: Jurnal Ilmiah
Kesehatan Masyarakat, 1(1), 01–08. https://doi.org/10.55123/sehatmas.v1i1.15
Anisya Kurnia, K., Laily Hilmi, I., Singaperbangsa Karawang, U., Barat, J., &
Author, I. (2023). Journal of Pharmaceutical and Sciences |Volume 6|No.
Annisa Rahma Aryanti, Made Helen Susanti, Anjar Hermadi Saputro, Herayati,
Indah Puspita Sari, & Syahjoko Saputra, I. (2025). Perbandingan Metode
Ekstraksi Maserasi, Sokletasi, Dan Sonikasi Terhadap Nilai Rendemen Ekstrak
Rimpang Kunyit (Curcuma Longa L.). Journal of Chemistry
Sciences and Education, 2(01), 1–9. https://doi.org/10.69606/jcse.v2i01.237
Bhakti, T. S., Porwanti, R., Pribadi, P., & Rahmawati, D. (2024). Total Plate
Number Test at 0.5 McFarland Standard in Escherichia coli Culture. Bioma :
Berkala Ilmiah Biologi, 25(2), 94 97. https://doi.org/10.14710/bioma.2023.520
57
Chaisaeng, S., Chopjitt, P., Kasemsiri, P., Putthanachote, N., Boueroy, P.,
Takeuchi, D., Akeda, Y., Hamada, S., & Kerdsin, A. (2024). High prevalence of
ESBL-producing E. coli phylogroup B2 clinical isolates in northeaste
Thailand. BMC Microbiology, 24(1), 425. https://doi.org/10.1186/s12866-024-
03582-0
Cheung, G. Y. C., Bae, J. S., & Otto, M. (2021). Pathogenicity and virulence of
Staphylococcusaureus.Virulence,12(1),547–569.
https://doi.org/10.1080/21505594.2021.1878688
Dong, Y., Yang, C., Zhong, W., Shu, Y., Zhang, Y., & Yang, D. (2022).
Antibacterial effect and mechanism of anthocyanin from Lycium ruthenicum Murr.
Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.974602
Elzuhria A, N., Kaffah, N. S., N, N. R., Hanidah, U., S, A. M., Nabilla, F. A.,
Azkadhafina, F., Hidayat, T. A., Putri, N. F., Zahra, P. A., Setiawan, T., &
Buulolo, F. (2023). ANTIBIOTICS SENSITIVITY TEST DIFFUSION AND
DILUTION METHODS. Journal of Research in Pharmacy and Pharmaceutical
Sciences, 2(1), 38–47. https://doi.org/10.33533/jrpps.v2i1.7027
Fuad Abdilah, & Kurniawan. (2022). morfologi s aureus . Habboush, Y., &
Guzman, N. (2025). Antibiotic Resistance.
Hasanpour, A. H., Sepidarkish, M., Mollalo, A., Ardekani, A., Almukhtar, M.,
Mechaal, A., Hosseini, S. R., Bayani, M., Javanian, M., & Rostami, A. (2023).
The global prevalence of methicillin-resistant Staphylococcus aureus
colonization in residents of elderly care centers: a systematic review and metaanalysis.
Antimicrobial Resistance & Infection Control, 12(1), 4.
https://doi.org/10.1186/s13756-023-01210-6
Izquierdo-Vega, J. A., Castillo-Juarez, R. J., Sánchez-Gutiérrez, M., Ares, M. A., &
De La Cruz, M. A. (2023). A Mini-Review of Enteroaggregative
Escherichia coli with a Specific Target on the Virulence Factors Controlled by the
AggR Master Regulator. Polish Journal of Microbiology, 72(4), 347–354.
https://doi.org/10.33073/pjm-2023-037
Ji, Q.-Y., Wang, W., Yan, H., Qu, H., Liu, Y., Qian, Y., & Gu, R. (2023). The Effect
of Different Organic Acids and Their Combination on the Cell Barrier and
Biofilm of Escherichia coli. Foods, 12(16), 3011. https://doi.org/10.3390/foods12163011
Jung, B., & Hoilat, G. J. (2025). MacConkey Medium.
Karahan, F. (2020a). Morphology, anatomy, palynology and achene
micromorphology of Bellis L. (Asteraceae) species from Turkey. Acta Botanica
Croatica, 79(1), 59–67. https://doi.org/10.37427/botcro-2020-006
Karahan, F. (2020b). Morphology, anatomy, palynology and achene
micromorphology of Bellis L. (Asteraceae) species from Turkey. Acta
Botanica Croatica, 79(1), 59–67. https://doi.org/10.37427/botcro-2020-006
Lasmini, T., Anisha, T., Kesehatan, A., Kesehatan, A., & Paul, J. (n.d.). POLA
KEPEKAAN ANTIBIOTIK ISOLAT BAKTERI ENTEROBACTERIACEAE
KONTAMINAN PADA SIKAT GIGI. Cross- Border, 3, 371–378.
Lee, J.-E., Jayakody, J. T. M., Kim, J.-I., Jeong, J.-W., Choi, K.-M., Kim, T.-S.,
Seo, C., Azimi, I., Hyun, J.-M., & Ryu, B.-M. (2024). The Influence of Solvent
Choice on the Extraction of Bioactive Compounds from Asteraceae: A
Comparative Review. Foods (Basel, Switzerland), 13(19).
https://doi.org/10.3390/foods13193151
Li, J., & Monje-Galvan, V. (2024). Effect of Glycone Diversity on the Interaction of
Triterpenoid Saponins and Lipid Bilayers. ACS Applied Bio Materials, 7(2),
553–563. https://doi.org/10.1021/acsabm.2c00928
Liu, Y., Zhu, J., Liu, Z., Zhi, Y., Mei, C., & Wang, H. (2025). Flavonoids as
Promising Natural Compounds for Combating Bacterial Infections.
International Journal of Molecular Sciences, 26(6), 2455.
https://doi.org/10.3390/ijms26062455
Lobiuc, A., Pavăl, N.-E., Mangalagiu, I. I., Gheorghiță, R., Teliban, G.-C.,
Amăriucăi-Mantu, D., & Stoleru, V. (2023). Future Antimicrobials: Natural and
Functionalized Phenolics. Molecules, 28(3), 1114.
https://doi.org/10.3390/molecules28031114
Martak, D., Henriot, C. P., Broussier, M., Couchoud, C., Valot, B., Richard, M.,
Couchot, J., Bornette, G., Hocquet, D., & Bertrand, X. (2020). High Prevalence
of Human-Associated Escherichia coli in Wetlands Located in Eastern France.
Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.552566
Michalik, M., Podbielska-Kubera, A., & Dmowska-Koroblewska, A. (2025).
Antibiotic Resistance of Staphylococcus aureus Strains—Searching for New
Antimicrobial Agents—Review. Pharmaceuticals, 18(1), 81.
https://doi.org/10.3390/ph18010081
Mousa, N., Aiesh, B. M., Jomaa, R., Zouneh, Y., Namrouti, A., Abutaha, A., Al-
Jabi, S. W., Sabateen, A., & Zyoud, S. H. (2025). Antibiotic resistance profiles
and risk factors of multidrug-resistant Escherichia coli in a large tertiary care
hospital in a low- and middle-income country. Scientific
Reports, 15(1), 26667. https://doi.org/10.1038/s41598-025-12384-x
Mueller, M., & Tainter, C. R. (2025a). Escherichia coli Infection. Mueller, M., &
Tainter, C. R. (2025b). Escherichia coli Infection. Mueller, M., & Tainter, C. R.
(2025c). Escherichia coli Infection.
Mulyawan, B., Widodo, A. D. W., & Endraswari, P. D. (2024). Comparison of
generation time between Escherichia coli non- extended spectrum betalactamase
(non-ESBL) and ESBL on ciprofloxacin and tetracycline induction:
Experimental research. Bali Medical Journal, 13(1), 811–815.
https://doi.org/10.15562/bmj.v13i1.4890
Nandhini, P., Kumar, P., Mickymaray, S., Alothaim, A. S., Somasundaram, J., &
Rajan, M. (2022). Recent Developments in Methicillin-Resistant
Staphylococcus aureus (MRSA) Treatment: A Review. Antibiotics, 11(5), 606.
https://doi.org/10.3390/antibiotics11050606
Nurjanah, G. S., Cahyadi, A. I., & Windria, S. (2020). ESCHERICHIA COLI
RESISTANCE TO VARIOUS KINDS OF ANTIBIOTICS IN ANIMALS
AND HUMANS: A LITERATURE STUDY. Indonesia Medicus Veterinus, 9(6),
970–983. https://doi.org/10.19087/imv.2020.9.6.970
Puji Natalia Kristiani Sitorus, Aina Azzahra, Dimas Ramadhan Lubis, Karen
Zefanya Gulo, Puan Adila, & Tri Amanda Siregar. (2024). Keberadaan
Esherichia Coli Pada Berbagai Jenis Air. Algoritma : Jurnal Matematika, Ilmu
Pengetahuan Alam, Kebumian Dan Angkasa, 2(5), 32–29.
https://doi.org/10.62383/algoritma.v2i5.116
Rahmah, W. N., Ramdhani, F. H., & Hidayani, A. (2024). Gambaran Hasil Uji
Sensitivitas Antibiotik Terhadap Bakteri Escherichia coli dengan Metode DISC
dan Sumuran. Jurnal Surya Medika, 10(2), 344–348. https://doi.org/10.33084/jsm.v10i2.7495
Rahmawati, M. D., Suardana, I. W., & Dharmawan, N. S. (2025).
IDENTIFICATION OF STAPHYLOCOCCUS SP. ISOLATES FROM PIG
TONSILS BASED ON MANNITOL SALT AGAR TEST. Buletin Veteriner
Udayana, 366–377. https://doi.org/10.24843/bulvet.2025.v17.i02.p15
Ramirez, D. M., & Schweizer, F. (2025). Development of polymyxin- and
aminoglycoside-based outer membrane permeabilizers: a review. Frontiers in
Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1625300
Rasquel-Oliveira, F. S., Ribeiro, J. M., Martelossi-Cebinelli, G., Costa, F. B.,
Nakazato, G., Casagrande, R., & Verri, W. A. (2025). Staphylococcus aureus in
Inflammation and Pain: Update on Pathologic Mechanisms. Pathogens, 14(2),
185. https://doi.org/10.3390/pathogens14020185
Rayhan, M., Rahman, F., Stat Oksi, S., Hadi, A., & Stat, S. (2025). Data Resistensi
Antibiotik Indonesia Tahun 2024: Pola Patogen dan Antibiogram Penyusun.
Rente, D., Cvjetko Bubalo, M., Panić, M., Paiva, A., Caprin, B., Radojčić
Redovniković, I., & Duarte, A. R. C. (2022). Review of deep eutectic systems
from laboratory to industry, taking the application in the cosmetics industry as
an example. Journal of Cleaner Production, 380, 135147.
https://doi.org/10.1016/j.jclepro.2022.135147
Riset, A., Khairunnisa, N., Lisa Yuniati, K., St Fahirah Arsal, A., & Faisal Syamsu,
R. (2023). FAKUMI MEDICAL JOURNAL Efektifitas Ekstrak Daun
Kemangi & Ekstrak Daun Sirih Merah sebagai Anti Mikroba Staphylococcus
aureus Penyebab Furunkle. Fakumi Medical Journal: Jurnal Mahasiswa
Kedokteran, 3(2).
Schoch, C. L., Ciufo, S., Domrachev, M., Hotton, C. L., Kannan, S., Khovanskaya,
R., Leipe, D., Mcveigh, R., O’Neill, K., Robbertse, B., Sharma, S., Soussov,
V., Sullivan, J. P., Sun, L., Turner, S., & Karsch- Mizrachi, I. (2020). NCBI
Taxonomy: a comprehensive update on curation, resources and tools.
Database, 2020. https://doi.org/10.1093/database/baaa062
Shijila Rani, A. S., Babu, S., Anbukumaran, A., Veeramani, S., Ambikapathy, V.,
Gomathi, S., & Senthilkumar, G. (2021). Prospective Approaches of
Pseudonocardia alaniniphila Hydrobionts for Litopenaeus vannamei. In Advances in Probiotics (pp. 327–348). Elsevier. https://doi.org/10.1016/B978-0-12-
822909-5.00021-6
Sizar, O., Leslie, S. W., & Unakal, C. G. (2025). Gram-Positive Bacteria. Taylor,
T. A., & Unakal, C. G. (2025). Staphylococcus aureus Infection.
Thiago H. (n.d.). Phytochemical Profile and Qualifi cation of Biological Activity of
an Isolated Fraction of Bellis perennis.
Touaitia, R., Mairi, A., Ibrahim, N. A., Basher, N. S., Idres, T., & Touati, A.
(2025a). Staphylococcus aureus: A Review of the Pathogenesis and Virulence
Mechanisms.Antibiotics,14(5),470.https://doi.org/10.3390/antibiotics14050470
Touaitia, R., Mairi, A., Ibrahim, N. A., Basher, N. S., Idres, T., & Touati, A. (2025b).
Staphylococcus aureus: A Review of the Pathogenesis and Virulence
Mechanisms.Antibiotics,14(5),470.https://doi.org/10.3390/antibiotics14050470
Tuon, F. F., Suss, P. H., Telles, J. P., Dantas, L. R., Borges, N. H., & Ribeiro, V. S.T.
(2023). Antimicrobial Treatment of Staphylococcus aureus Biofilms.
Antibiotics, 12(1), 87. https://doi.org/10.3390/antibiotics12010087
Wari Rahman, I., Arfani, N., & Veronica Tadoda, J. (2023). Deteksi Bakteri MRSA
Methicillin-Resistant Staphylococcus aureus pada Sampel Darah Pasien
Rawat Inap. https://journal.unhas.ac.id/index.php/jai2
WHO. (2025). Global antibiotic resistance surveillance report 2025 WHO Global
Antimicrobial Resistance and Use Surveillance System (GLASS).
Wibisono, F. J., SUMIARTO, B., UNTARI, T., Effendi, M. H., PERMATASARI,
D. A., & WITANINGRUM, A. M. (2020). Short Communication: The presence of
extended-spectrum beta-lactamase (ESBL) producing Escherichia coli on layer
chicken farms in Blitar Area, Indonesia.Biodiversitas Journal of Biological
Diversity, 21(6). https://doi.org/10.13057/biodiv/d210638
World Health Organization. (2025). Proportion of bloodstream infection due to
methicillin-resistant Staphylococcus aureus (MRSA) (%).
Yahav, D., Shepshelovich, D., & Tau, N. (2021). Cost Analysis of New Antibiotics
to Treat Multidrug-Resistant Bacterial Infections: Mind the Gap. Infectious
Diseases and Therapy, 10(1), 621–630. https://doi.org/10.1007/s40121-021-
00412-y
Agus Rahmadi, (2023). Albien, A.-L., & Stark, T. D. (2023). (Bio)active Compounds in Daisy Flower
(Bellis perennis). Molecules (Basel, Switzerland), 28(23).
https://doi.org/10.3390/molecules28237716
Belay, W. Y., Getachew, M., Tegegne, B. A., Teffera, Z. H., Dagne, A., Zeleke,
T. K., Abebe, R. B., Gedif, A. A., Fenta, A., Yirdaw, G., Tilahun, A., &
Aschale, Y. (2024). Mechanism of antibacterial resistance, strategies and
next-generation antimicrobials to contain antimicrobial resistance: a review.
Frontiers in Pharmacology, 15. https://doi.org/10.3389/fphar.2024.1444781
Karić, E., Horozić, E., Pilipović, S., Dautović, E., Ibišević, M., Džambić, A.,
Čeliković, S., & Halilčević, A. (2023). Tyrosinase Inhibition, Antioxidant and
Antibacterial Activity of Commercial Daisy Extract (Bellis perennis). Journal
ofPharmaceuticalResearchInternational,35(5),13–19.
https://doi.org/10.9734/jpri/2023/v35i57325
Mueller, M., & Tainter, C. R. (2025). Escherichia coli Infection.
Sizar, O., Leslie, S. W., & Unakal, C. G. (2026). Gram-Positive Bacteria.
Whelan, S., Lucey, B., & Finn, K. (2023). Uropathogenic Escherichia coli
(UPEC)-Associated Urinary Tract Infections: The Molecular Basis for
Challenges to Effective Treatment. Microorganisms, 11(9), 2169.
https://doi.org/10.3390/microorganisms11092169
Yu, H., Liu, X., Zhang, S., Lei, Y., Zou, B., Xie, P., Sun, B., & Zang, M. (2025).
Advances in antibacterial strategies of organic acids coupled with cold
sterilization methods for controlling bacterial contamination in beef carcasses
and cuts. Critical Reviews in Food Science and Nutrition, 1–16.
https://doi.org/10.1080/10408398.2025.2534162
citation:   CUT ASHILAH HAURA, ASHILAH  (2026) SKRIPSI-2210015020-CUT ASHILAH HAURA-REPOSITORY.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. Hamka.   
document_url: http://repository.uhamka.ac.id/id/eprint/56373/1/SKRIPSI-2210015020-CUT%20ASHILAH%20HAURA-REPOSITORY.pdf