%I INTERNATIONAL RESEARCH JOURNAL OF PHARMACY
%L repository5004
%A Supandi Supandi Supandi
%T PRINCIPAL COMPONENT ANALYSIS (PCA)-COMBINED HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) FOR DIFFERENTIATION OF BOVINE AND PORCINE GELATIN IN VITAMIN C GUMMIES
%X Gelatin is a polymer derived from skin and bone of vertebrate, bovine and porcine. Gelatin is used as a gelling agent in the pharmaceutical and food
products including in the preparation of vitamin C gummies. Porcine gelatin is forbidden to consume by Muslim and Jewish. The aim of this study was
to differentiate and classify of bovine and porcine gelatin in vitamin C gummies using a combination method of High-Performance Liquid
Chromatography (HPLC) and Principal Component Analysis (PCA). The analysis procedure involved complete hydrolysis of samples by 6N
hydrochloride acid in order to release their amino acid residues. To get an amino acid composition of gelatin, all of the samples were performed by
reversed-phase (RP) HPLC. The peak height of HPLC chromatogram was analyzed using PCA to classify both bovine and porcine gelatin. The Results
of PCA showed a clear distinction between bovine and porcine gelatin in vitamin C gummies. Hence, this method could be successfully used for
differentiation of bovine and porcine gelatin in vitamin C gummies.
%J PRINCIPAL COMPONENT ANALYSIS (PCA)-COMBINED HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) FOR DIFFERENTIATION OF BOVINE AND PORCINE GELATIN IN VITAMIN C GUMMIES