relation: http://repository.uhamka.ac.id/id/eprint/4862/
title: Determination of Captopril in Rat Plasma by  LC-MS/MS in Presence of Apigenin
creator: Siska, Siska
subject: Q Science (General)
description: To determine and validate of captopril in presence of apigenin by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in rat plasma. The captopril and apigenin were extracted from rat plasma by protein  precipitation with acetonitrile. Sample containing captopril and apigenin were analyzed by using LC-MS/MS with  C18 column Acquity® (100 mm×21 mm), 1.7 m particles size column at 40 C. The gradient system of mobile  phase composition was a mixture of 0.1% formic acid and acetonitrile (60; 40 v/v), with flow rate 0.3 ml/second.  Mass detection was performed on Waters Xevo Triple Quadrupole equipped with an electrospray ionization  (ESI) source in positive ion mode in the multiple reaction monitoring (MRM) modes. Captopril was detected  at m/z 415 > 21616, apigenin was detected at m/z 27113 > 15307 and propranolol an internal standard was  detected at m/z 260 > 18317. Results: The method was validated according to EMEA guidelines which showed  good reproducibility and linearity of 0.9992, the LLOQ were 10 ng/ml for captopril. The precision (%CV) value of  Within-run and between-run analysis is 3.90–10.90% and 3.77–8.13% whereas the accuracy (%diff) of captopril  was less than 20%. Stability studies revealed that captopril has been stable for 6 hours at room temperature,  three freeze-thaw cycles, and at least 120 days at −40 C. Conclusion: The developed LC-MS/MS method  is valid to evaluate captopril in present of apigenin in vitro and meet the requirement of linearity, accuracy,  selectivity, precision, matrix effect, and stability according to EMEA 2011
publisher: American Scientific Publishers
date: 2017
type: Article
type: PeerReviewed
format: text
language: en
identifier: http://repository.uhamka.ac.id/id/eprint/4862/2/10.%20ASL_2017_OK.pdf
format: text
language: en
identifier: http://repository.uhamka.ac.id/id/eprint/4862/5/ASL_2017_Determination%20of%20Captopril%20in%20Rat%20Plasma%20by%20LC-MS_MS%20in%20Presence%20of%20Apigenin.pdf
format: text
language: en
identifier: http://repository.uhamka.ac.id/id/eprint/4862/22/10.%20PR%20ASL%202017.pdf
format: text
language: en
identifier: http://repository.uhamka.ac.id/id/eprint/4862/23/10.%20Bukti%20Korespondensi_ASL_2017.pdf
identifier:   Siska, Siska  (2017) Determination of Captopril in Rat Plasma by LC-MS/MS in Presence of Apigenin.  Advanced Science Letters, 23 (12).  pp. 12447-12450.  ISSN ISSN: 1936-6612 (Print); 1936-7317 (Online).     
relation: https://www.ingentaconnect.com/content/asp/asl/2017/00000023/00000012/art00179;jsessionid=a96ge5jmefm8d.x-ic-live-02