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primary topic: Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time

Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time
type: AcademicArticle
type: Article
abstract: DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins. Contamination of RNA, carbohydrates, lipids, and proteins can increase DNA purity. DNA purity was measured using a NanoDrop 2000 spectrophotometer measured by the absorbance ratio at 260 nm and 280 nm wavelengths. Good quality DNA will have an A260/A280 ratio of 1.7–2.0 and a concentration > 0.03 pg. This study aimed to obtain the appropriate DNA extraction method for fresh meat samples (a mixture of rat and chicken meat). This research consisted of two stages: the DNA extraction stage using the Progenus EasyFast™ Extraction Kit for Meat Products and the amplification stage using the EASYFAST™ Rat Detection Kit. This study used 16 samples of a mixture of rat meat and chicken with concentrations of rat meat: 5, 10, 15, and 20%. At the extraction stage, the incubation time was optimized for 15, 30, 45 minutes, and 1 hour. The results showed that the one hour incubation had a lowest CT value in the results of PCR amplification.
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time (Text)
subject: Fakultas Farmasi dan Sains

Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time (Text)
type: Document
has file: IJHAR-Artikel Optimization of DNA Extraction.pdf
has part: IJHAR-Artikel Optimization of DNA Extraction.pdf

Indonesian Journal of Halal Research
type: Organization

Indonesian Journal of Halal Research
type: Collection

Permanasari Etin Diah
type: Person
given name: Permanasari
family name: Etin Diah

Hadi Hadi Sunaryo
type: Person
given name: Hadi
family name: Hadi Sunaryo

Azmah Nikmatullah Nurul
type: Person
given name: Azmah Nikmatullah
family name: Nurul

Adia Adia Putra Wirman
type: Person
given name: Adia
family name: Adia Putra Wirman

UHAMKA
type: Organization

HTML Summary of #48490 Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time
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primary topic: Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time

Fakultas Farmasi dan Sains
type: Concept