eprintid: 41517
rev_number: 7
eprint_status: archive
userid: 2310
dir: disk0/00/04/15/17
datestamp: 2025-01-30 04:09:39
lastmod: 2025-01-30 04:09:39
status_changed: 2025-01-30 04:09:39
type: thesis
metadata_visibility: show
creators_name: Maifitrianti, maifitrianti
creators_orcid: 0000-0002-9233-1557
title: PENGARUH EKSTRAK ETANOL 96% DAUN UNGU (Graptophyllum
pictum (L.) Griff.) TERHADAP PENURUNAN KADAR MDA PADA
OTAK TIKUS PUTIH JANTAN MODEL DEMENSIA
ispublished: pub
subjects: RS
divisions: 48201
abstract: PENGARUH EKSTRAK ETANOL 96% DAUN UNGU (Graptophyllum
pictum (L.) Griff.) TERHADAP PENURUNAN KADAR MDA PADA
OTAK TIKUS PUTIH JANTAN MODEL DEMENSIA
MUHAMMAD ALIF HUDA
1804015252
Ekstrak etanol 96% daun ungu mengandung senyawa flavonoid, alkaloid, fenol,
triterpenoid dan saponin yang diduga memiliki khasiat sebagai antioksidan.
Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol
96% daun ungu terhadap kadar malondialdehid (MDA) pada otak tikus putih
jantan yang diinduksi dengan skopolamin sebagai pemodelan hewan dengan
gangguan kognitif (demensia). Hewan percobaan dibagi menjadi 5 kelompok
yaitu kelompok kontrol normal, kelompok kontrol negatif, kelompok kontrol
positif, kelompok dosis 200mg/kgBB dan kelompok dosis 400mg/kg BB.
Demensia pada tikus diinduksi dengan menggunakan skopolamin dosis 20 mg/kg
BB i.p. Hewan diuji secara (moriz water maze). Suspensi ekstrak diberikan secara
oral selama 15 hari. Satu hari setelah pemberian ekstrak dilakukan pengambilan
otak dan dibuat homogenat. Kadar MDA diukur dengan metode TBARs
(Thiobarbituric Acid-Reactif Substance). Data kadar MDA dibaca secara statistik
dengan ANOVA satu arah dan dilanjutkan dengan uji tukey. Hasil penelitian
menunjukan bahwa pemberian ekstrak etanol 96% daun ungu dengan dosis 200
dan 400 dapat menurunkan kadar MDA secara signifikan (p<0,05). Kelompok
ekstrak etanol 96% daun ungu dosis 400mg/kg BB memiliki efek penurunan
kadar MDA lebih baik dibandingkan dengan dosis 200mg/kg BB.
Kata Kunci: ekstrak etanol 96% daun ungu, MDA, otak tikus, demensia,
date: 2024
date_type: completed
full_text_status: public
institution: UNIVERSITAS MUHAMMADIYAH PROF. DR. HAMKA
department: Fakultas Farmasi Dan Sains
thesis_type: bachelor
thesis_name: bphil
referencetext: Al-Kuraishy, H. M., & Al-Gareeb, A. I. (2020). Research paper: Citicoline
improves human vigilance and visual working memory: The role of neuronal
activation and oxidative stress. Basic and Clinical Neuroscience, 11(4), 423–
432. https://doi.org/10.32598/bcn.11.4.1097.1
Ali, I. R., Lestari, R. D., & Andriana, D. (2020). Perbandingan Efek Perasan
Lidah Buaya (Aloe Vera) Dengan Povidone Iodine Terhadap Kadar
Superoxid Dismutase Dan Malondialdehid Serum Tikus Wistar Dengan Luka
Sayat. Jurnal Kedokteran Komunitas, 8(1), 1–8.
Andrian, K., Rochmah, N., & Arifah, F. (2018). Karakterisasi Parameter Spesifik
Dan Non Spesifik Ekstrak Etanol Dan Teratai (Nelumbium nelumbo D.).
Prosiding Seminar Nasional Sains, Teknologi Dan Analisis Ke-1 2018, 197–
205. https://prosidingonline.iik.ac.id/index.php/sintesis/article/view/63
Arvanitakis, Z., Shah, R. C., & Bennett, D. A. (2019). Diagnosis and Management
of Dementia: Review. JAMA - Journal of the American Medical Association,
322(16), 1589–1599. https://doi.org/10.1001/jama.2019.4782
Aski, M. L., Rezvani, M. E., Khaksari, M., Hafizi, Z., Pirmoradi, Z., Niknazar, S.,
& Mehrjerdi, F. Z. (2018). Neuroprotective effect of berberine chloride on
cognitive impairment and hippocampal damage in experimental model of
vascular dementia. Iranian Journal of Basic Medical Sciences, 21(1), 53–58.
https://doi.org/10.22038/ijbms.2017.23195.5865
Ayala, A., Muñoz, M. F., & Argüelles, S. (2014). Lipid peroxidation: Production,
metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-
2-nonenal. Hindawi Publishing Corporation, 2014, 1–31.
https://doi.org/10.1155/2014/360438
Butterfield, D. A., Griffin, S., Munch, G., & Pasinetti, G. M. (2002). Amyloid β-
peptide and amyloid pathology are central to the oxidative stress and
inflammatory cascades under which Alzheimer’s disease brain exists.
Journal of Alzheimer’s Disease, 4(3), 193–201. https://doi.org/10.3233/JAD-
2002-4309
Cheon, S. Y., Koo, B. N., Kim, S. Y., Kam, E. H., Nam, J., & Kim, E. J. (2021).
Scopolamine promotes neuroinflammation and delirium-like
neuropsychiatric disorder in mice. Scientific Reports, 11(1), 1–13.
https://doi.org/10.1038/s41598-021-87790-y
Darwatik, Ratnawati, R., Rianawati, S., & Sarwono, I. (2018). Pengaruh
Antosianin Ubi Ungu terhadap TNF-α , Apoptosis dan Memori Spasial
Hipokampus Tikus Model Diabetes Melitus Effect of Purple Sweet Potato
Anthocyanin towards TNF-α , Apoptosis , and Spatial Memory in
Hippocampus of Rat Model of Diabetes Mellitus. Jurnal Kedokteran
Brawijaya, 30(1), 12–18.
Departemen Kesehatan RI. (2000). Parameter Standar Umum Ekstrak Tanaman
Obat. In Departemen Kesehatan RI (Vol. 1, pp. 10–11).
Dwita, L. P., Iwo, M. I., Elfahmi, & Mauludin, R. (2023). Brain Antioxidant
Properties of Piper Cubeba L. Extracts and Essential Oil. Farmacia, 71(2),
296–302. https://doi.org/10.31925/farmacia.2023.2.9
Gareri, P., Castagna, A., Cotroneo, A. M., Putignano, S., De Sarro, G., & Bruni,
A. C. (2015). The role of citicoline in cognitive impairment
Pharmacological characteristics, possible advantages, and doubts for an old
drug with new perspectives. Clinical Interventions in Aging, 10, 1421–1429.
https://doi.org/10.2147/CIA.S87886
Haider, S., Tabassum, S., & Perveen, T. (2016). Scopolamine-induced greater
alterations in neurochemical profile and increased oxidative stress
demonstrated a better model of dementia: A comparative study. Brain
Research Bulletin, 127, 234–247.
https://doi.org/10.1016/j.brainresbull.2016.10.002
Jiangseubchatveera, N., Liawruangrath, S., Teerawutgulrag, A., Santiarworn, D.,
Pyne, S. G., & Liawruangrath, B. (2017). Phytochemical screening, phenolic
and flavonoid contents, antioxidant and cytotoxic activities of
Graptophyllum pictum (L.) Griff. Chiang Mai Journal of Science, 44(1),
193–202.
Makkiyah, F., Rahmi, E. P., Revina, R., Susantiningsih, T., & Setyaningsih, Y.
(2021). Graptophyllum pictum (l.) griff. (syn: Justicia picta linn.) and its
effectiveness: A well-known indonesian plant. Pharmacognosy Journal,
13(3), 835–838. https://doi.org/10.5530/pj.2021.13.106
Manoi, F. (2011). Analisa Fitokimia dan Kandungan Bahan Aktif dari Lima
Aksesi Tanaman Handeuleum ( Graptophyllum pictum ( L .) Griff ) Analysis
Phytochemical and Active Component Compound from Five Accession
Graptophyllum pictum ( L .) Griff ). Jurnal Penelitian Pertanian Terapan,
11(1), 15–24.
Mulianto, N. (2020). Malondialdehid sebagai Penanda Stres Oksidatif pada
Berbagai Penyakit Kulit. Cermin Dunia Kedokteran, 47(1), 39–44.
Neff, R. T., Jindal, R. M., Yoo, D. Y., Hurst, F. P., Agodoa, L. Y., & Abbott, K.
C. (2009). Analysis of USRDS: Incidence and risk factors for Pneumocystis
jiroveci pneumonia. Transplantation, 88(1), 135–141.
https://doi.org/10.1097/TP.0b013e3181aad256
Salim, R. (2018). Uji Aktivitas Antioksidan Infusa Daun Ungu Dengan Metoda
DPPH (1,1- diphenil- 2-picrylhidrazil). Jurnal Katalisator, 3(2), 153.
https://doi.org/10.22216/jk.v3i2.3372
Sartika, S., & Indradi, R. B. (2021). Pharmacological Activities of Graptophyllum
pictum L. Griff. Indonesian Journal of Biological Pharmacy, 1(2), 88–96.
Shabani, S., & Mirshekar, M. A. (2018). Diosmin is neuroprotective in a rat
model of scopolamine-induced cognitive impairment. Biomedicine and
Pharmacotherapy, 108(September), 1376–1383.
https://doi.org/10.1016/j.biopha.2018.09.127
Sinaga, F. A. (2016). Stress oksidatif dan status antioksidan pada aktivitas fisik
maksimal. Jurnal Generasi Kampus, 9(2), 176–189.
Tahseen, M. A., Bhatnagar, A., & Bhatnagar, A. (2023). Phytochemical and
pharmacological investigations of Graptophyllum pictum leaves. Journal of
Pharmacognosy and Phytochemistry, 12(6), 252–266.
Tsikas, D., Rothmann, S., Schneider, J. Y., Suchy, M. T., Trettin, A., Modun, D.,
Stuke, N., Maassen, N., & Frölich, J. C. (2016). Development, validation and
biomedical applications of stable-isotope dilution GC-MS and GC-MS/MS
techniques for circulating malondialdehyde (MDA) after pentafluorobenzyl
bromide derivatization: MDA as a biomarker of oxidative stress and its
relation to 1. Journal of Chromatography B: Analytical Technologies in the
Biomedical and Life Sciences, 1019, 95–111.
https://doi.org/10.1016/j.jchromb.2015.10.009
Werdhasari, A. (2014). Peran Antioksidan Bagi Kesehatan. Jurnal Biotek
Medisiana Indonesia, 03(2), 59–68.
Zaki, H. F., Abd-El-Fattah, M. A., & Attia, A. S. (2014). Naringenin protects
against scopolamine-induced dementia in rats. Bulletin of Faculty of
Pharmacy, Cairo University, 52(1), 15–25.
https://doi.org/10.1016/j.bfopcu.2013.11.001
Zalukhu, M. L., Phyma, A. R., & Pinzon, R. T. (2016). Proses Menua , Stres
Oksidatif , dan Peran Antioksidan. CDK-245 43(10), 733–736.
citation:   Maifitrianti, maifitrianti  (2024) PENGARUH EKSTRAK ETANOL 96% DAUN UNGU (Graptophyllum pictum (L.) Griff.) TERHADAP PENURUNAN KADAR MDA PADA OTAK TIKUS PUTIH JANTAN MODEL DEMENSIA.  Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PROF. DR. HAMKA.   
document_url: http://repository.uhamka.ac.id/id/eprint/41517/1/FS03-240110.pdf