<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND\r\n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID\r\nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY"^^ . "Objective: 6-mercaptopurine (6-MP) is a chemotherapeutic agent in the antimetabolite class. It has to go through the metabolic pathway to form\r\n6-methyl MP (6-MMP). This study aimed to obtain an optimum and validated method for the analysis of 6-MP and 6-MMP in dried blood spot (DBS)\r\nsamples simultaneously and to evaluate the potential for future drug concentration monitoring in DBS samples.\r\nMethods: The quality control and calibration curves were made by spotting 40 µL blood on DBS paper and dried for 3 hrs. DBS papers were cut with a\r\ndiameter of 8 mm and extracted with acetonitrile-methanol (1:3) containing internal standard 5-fluorouracil (5-FU). Separation was performed with\r\nwaters acquity ultra performance liquid chromatography BEH C18 column of 1.7 μm (2.1×100 mm) with a mobile phase consisting of 0.1% formic\r\nacid in water 0.1% formic acid in acetonitrile with gradient elution and a flow rate of 0.2 mL/minute. Mass detection was performed using Waters\r\nXevo TQD with positive electrospray ionization (ESI) for 6-MP and 6-MMP and negative ESI for 5-FU in the multiple reaction monitoring mode.\r\nResults: The detection rates of 6-MP, 6-MMP, and 5-FU were 153.09>119.09, 167.17>126.03, and 129.09>42.05, respectively. This method was linear\r\nwith the range at 26-1000 ng/mL for 6-MP and 13-500 ng/mL for 6-MMP with consecutive r≥0.998 and ≥0.999, respectively. The % relative error\r\nvalue and % relative standard deviation for accuracy and precision of intraday and interday were not more than 15% and not more than 20% at the\r\nlower limit of quantification concentration, respectively.\r\nConclusions: This method fulfilled the requirements of selectivity, linearity, carry over, and matrix effects referring to the European Medicines Agency\r\nguidelines."^^ . . . "International Journal of Applied Pharmaceutics"^^ . . . "09757058" . . . . . . . "Supandi"^^ . "Supandi"^^ . "Supandi Supandi"^^ . . . . . . "SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND\r\n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID\r\nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY (Text)"^^ . . . "supandi_IJAP_2017.pdf"^^ . . . "SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND\r\n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID\r\nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND\r\n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID\r\nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND\r\n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID\r\nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY (Other)"^^ . . . . . . "preview.jpg"^^ . . . "SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND\r\n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID\r\nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY (Other)"^^ . . . . . . "medium.jpg"^^ . . . "SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND\r\n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID\r\nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #382 \n\nSIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTOPURINE AND \n6-METHYLMERCAPTOPURINE IN DRIED BLOOD SPOT USING ULTRA PERFORMANCE LIQUID \nCHROMATOGRAPHY TANDEM MASS SPECTROMETRY\n\n" . "text/html" . .