eprintid: 381
rev_number: 8
eprint_status: archive
userid: 587
dir: disk0/00/00/03/81
datestamp: 2017-12-12 06:56:06
lastmod: 2017-12-12 06:56:06
status_changed: 2017-12-12 06:56:06
type: article
metadata_visibility: show
creators_name: Supandi, Supandi
creators_id: supandiuhamka@gmail.com
title: Simultaneous Analytical Method Of 6-Mercaptopurine and 6-
Methylmercaptopurine In-vitro Study With Bio-Sampling
Venipuncture and Dried Blood Spot
ispublished: pub
subjects: R1
subjects: RA0421
divisions: sch_med
abstract: Objective: 6-Mercaptopurine (6-MP) is a cancer chemotherapeutic agent. Metabolic pathway by
thiopurine S-methyltransferase (TPMT) to become 6-methylmercaptopurine (6-MMP).Bio-sampling is
required to obtainbiological sample, using two technique; invasive (venipuncture) and minimum
invasive(dried blood spot). This study aims to obtain an optimization and validation analysis 6-MP and
6-MMP in vitro study with bio-sampling venipuncture and dried blood spot (DBS). Method: Plasma from
venipuncture method extraction was done using dichloromethane. Separation was performed using
Waters HPLC, C18 SunfireTM column (5μm, 250 x 4.6 mm), with gradient elution, flow rate 1 mL/min
and detected at UV-PDA wavelength of 303 nm. Bio-sampling dried blood spot with DBS CAMAG®
paper diameter of 8 mm and extracted with acetonitrile-methanol (1:3). Separation was performed with
Waters LC-MS/MS UPLC C18 column (1.7 μm, 2.1 x 100 mm) with gradient elution, flow rate 0.2
mL/min. 5-fluorouracil (5-FU) was used as internal standard.Result: The method venipuncture was
linear at concentration range of 2-200 ng/mL for 6-MP and 20-2000 ng/mL for 6-MMP. The method dried
blood spot using Waters Xevo TQD for mass detection with positive electrospray ionization (ESI) for 6-
MP, 6-MMP and negative ESI for 5-FU in Multiple Reaction Monitoring mode. Linear with the range 26-
1000 ng/mL for 6-MP and 13-500 ng/mL for 6-MMP.Conclusion: The developed method is valid for 6-MP
and 6-MMP simultaneously in vitro from venipuncture using HPLC and from dried blood spot using LCMS/MS
and showed good selectivity, linearity, accuracy and precision, matrix effect and stability.
publisher: JGPT
official_url: http://jgpt.co.in/index.php/jgpt/
full_text_status: public
publication: Journal of Global Pharma Technology
refereed: TRUE
issn: 0975 -8542
citation:   Supandi, Supandi   Simultaneous Analytical Method Of 6-Mercaptopurine and 6- Methylmercaptopurine In-vitro Study With Bio-Sampling Venipuncture and Dried Blood Spot.  Journal of Global Pharma Technology.   ISSN 0975 -8542     
document_url: http://repository.uhamka.ac.id/id/eprint/381/1/Supandi_jgpt_2017.pdf