<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Simultaneous Analytical Method Of 6-Mercaptopurine and 6-\r\nMethylmercaptopurine In-vitro Study With Bio-Sampling\r\nVenipuncture and Dried Blood Spot"^^ . "Objective: 6-Mercaptopurine (6-MP) is a cancer chemotherapeutic agent. Metabolic pathway by\r\nthiopurine S-methyltransferase (TPMT) to become 6-methylmercaptopurine (6-MMP).Bio-sampling is\r\nrequired to obtainbiological sample, using two technique; invasive (venipuncture) and minimum\r\ninvasive(dried blood spot). This study aims to obtain an optimization and validation analysis 6-MP and\r\n6-MMP in vitro study with bio-sampling venipuncture and dried blood spot (DBS). Method: Plasma from\r\nvenipuncture method extraction was done using dichloromethane. Separation was performed using\r\nWaters HPLC, C18 SunfireTM column (5μm, 250 x 4.6 mm), with gradient elution, flow rate 1 mL/min\r\nand detected at UV-PDA wavelength of 303 nm. Bio-sampling dried blood spot with DBS CAMAG®\r\npaper diameter of 8 mm and extracted with acetonitrile-methanol (1:3). Separation was performed with\r\nWaters LC-MS/MS UPLC C18 column (1.7 μm, 2.1 x 100 mm) with gradient elution, flow rate 0.2\r\nmL/min. 5-fluorouracil (5-FU) was used as internal standard.Result: The method venipuncture was\r\nlinear at concentration range of 2-200 ng/mL for 6-MP and 20-2000 ng/mL for 6-MMP. The method dried\r\nblood spot using Waters Xevo TQD for mass detection with positive electrospray ionization (ESI) for 6-\r\nMP, 6-MMP and negative ESI for 5-FU in Multiple Reaction Monitoring mode. Linear with the range 26-\r\n1000 ng/mL for 6-MP and 13-500 ng/mL for 6-MMP.Conclusion: The developed method is valid for 6-MP\r\nand 6-MMP simultaneously in vitro from venipuncture using HPLC and from dried blood spot using LCMS/MS\r\nand showed good selectivity, linearity, accuracy and precision, matrix effect and stability."^^ . . . "JGPT"^^ . . . "Journal of Global Pharma Technology"^^ . . . "09758542" . . . . . . . "Supandi"^^ . "Supandi"^^ . "Supandi Supandi"^^ . . . . . . "Simultaneous Analytical Method Of 6-Mercaptopurine and 6-\r\nMethylmercaptopurine In-vitro Study With Bio-Sampling\r\nVenipuncture and Dried Blood Spot (Text)"^^ . . . "Supandi_jgpt_2017.pdf"^^ . . . "Simultaneous Analytical Method Of 6-Mercaptopurine and 6-\r\nMethylmercaptopurine In-vitro Study With Bio-Sampling\r\nVenipuncture and Dried Blood Spot (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Simultaneous Analytical Method Of 6-Mercaptopurine and 6-\r\nMethylmercaptopurine In-vitro Study With Bio-Sampling\r\nVenipuncture and Dried Blood Spot (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Simultaneous Analytical Method Of 6-Mercaptopurine and 6-\r\nMethylmercaptopurine In-vitro Study With Bio-Sampling\r\nVenipuncture and Dried Blood Spot (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Simultaneous Analytical Method Of 6-Mercaptopurine and 6-\r\nMethylmercaptopurine In-vitro Study With Bio-Sampling\r\nVenipuncture and Dried Blood Spot (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Simultaneous Analytical Method Of 6-Mercaptopurine and 6-\r\nMethylmercaptopurine In-vitro Study With Bio-Sampling\r\nVenipuncture and Dried Blood Spot (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #381 \n\nSimultaneous Analytical Method Of 6-Mercaptopurine and 6- \nMethylmercaptopurine In-vitro Study With Bio-Sampling \nVenipuncture and Dried Blood Spot\n\n" . "text/html" . . . "R Medicine (General)"@en . . . "RA0421 Public health. Hygiene. Preventive Medicine"@en . .