relation: http://repository.uhamka.ac.id/id/eprint/381/
title: Simultaneous Analytical Method Of 6-Mercaptopurine and 6-  Methylmercaptopurine In-vitro Study With Bio-Sampling  Venipuncture and Dried Blood Spot
creator: Supandi, Supandi
subject: R Medicine (General)
subject: RA0421 Public health. Hygiene. Preventive Medicine
description: Objective: 6-Mercaptopurine (6-MP) is a cancer chemotherapeutic agent. Metabolic pathway by  thiopurine S-methyltransferase (TPMT) to become 6-methylmercaptopurine (6-MMP).Bio-sampling is  required to obtainbiological sample, using two technique; invasive (venipuncture) and minimum  invasive(dried blood spot). This study aims to obtain an optimization and validation analysis 6-MP and  6-MMP in vitro study with bio-sampling venipuncture and dried blood spot (DBS). Method: Plasma from  venipuncture method extraction was done using dichloromethane. Separation was performed using  Waters HPLC, C18 SunfireTM column (5μm, 250 x 4.6 mm), with gradient elution, flow rate 1 mL/min  and detected at UV-PDA wavelength of 303 nm. Bio-sampling dried blood spot with DBS CAMAG®  paper diameter of 8 mm and extracted with acetonitrile-methanol (1:3). Separation was performed with  Waters LC-MS/MS UPLC C18 column (1.7 μm, 2.1 x 100 mm) with gradient elution, flow rate 0.2  mL/min. 5-fluorouracil (5-FU) was used as internal standard.Result: The method venipuncture was  linear at concentration range of 2-200 ng/mL for 6-MP and 20-2000 ng/mL for 6-MMP. The method dried  blood spot using Waters Xevo TQD for mass detection with positive electrospray ionization (ESI) for 6-  MP, 6-MMP and negative ESI for 5-FU in Multiple Reaction Monitoring mode. Linear with the range 26-  1000 ng/mL for 6-MP and 13-500 ng/mL for 6-MMP.Conclusion: The developed method is valid for 6-MP  and 6-MMP simultaneously in vitro from venipuncture using HPLC and from dried blood spot using LCMS/MS  and showed good selectivity, linearity, accuracy and precision, matrix effect and stability.
publisher: JGPT
type: Article
type: PeerReviewed
format: text
language: en
identifier: http://repository.uhamka.ac.id/id/eprint/381/1/Supandi_jgpt_2017.pdf
identifier:   Supandi, Supandi   Simultaneous Analytical Method Of 6-Mercaptopurine and 6- Methylmercaptopurine In-vitro Study With Bio-Sampling Venipuncture and Dried Blood Spot.  Journal of Global Pharma Technology.   ISSN 0975 -8542     
relation: http://jgpt.co.in/index.php/jgpt/