%J Jurnal Farmasi Indonesia (PHARMACY)
%D 2014
%X Ortho, meta, and para phenylenediamine are prohibited ingredients of hair dye because they cause dermatitis, irritation, allergic reactions, and cancer. It is required a reliable and validated method to identify all three hair dyes simultaneously. The analysis method using High Performance Liquid Chromatography (HPLC) with a ultra violet detector has been developed to identify ortho, meta, and para phenylenediamine in hair dye preparations. HPLC analytical method for the identification of ortho, meta , and para phenylenediamine was optimized using methanol: water: 0.5% sodium ascorbate (50: 40: 10) as a solvent and phenyl-C18 column X-bridge (4.6 x 250 mm, 5 µm particle size) with phosphate buffer solution pH 10: methanol (95: 5) as mobile phase, with a flow rate of 1.0 mL / min and UV detector at 290 nm. After optimized, the methode was validated. It can be concluded that the analysis method is valid with the retention time of ortho, meta and para phenylenediamine are 1.092%, 1.049% and 0.817%, respectively, and the limit of detection of ortho, meta, and para phenylenediamine are 0.132 µg/g, 0.195 µg/g and 62.468 µg/g, respectively.
%N 01
%V 11
%I Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto
%T OPTIMASI DAN VALIDASI METODE ANALISIS IDENTIFIKASI ORTO, META, DAN PARA FENILENDIAMIN DALAM SEDIAAN PEWARNA RAMBUT SECARA KROMATOGRAFI CAIR KINERJA TINGGI
%A Supandi Supandi
%A Tashdiq Tashdiq Anwarulloh
%A Almawati Almawati Situmorang
%L repository351
%P 49-61