eprintid: 348
rev_number: 6
eprint_status: archive
userid: 5
dir: disk0/00/00/03/48
datestamp: 2017-10-10 10:20:11
lastmod: 2017-10-10 10:20:11
status_changed: 2017-10-10 10:20:11
type: article
metadata_visibility: show
creators_name: Supandi, Supandi
creators_name: Yahdiana Harahap, Yahdiana
creators_name: Nurul Azizah, Nurul
creators_name: Rizka Andalusia, Rizka
creators_id: supandiuhamka@gmail.com
corp_creators: Department of Pharmacy, Faculty of Pharmacy and Science, UHAMKA, Jakarta, Indonesia
corp_creators: Faculty of Pharmacy Universitas Indonesia, Depok, INDONESIA.
title: SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTO¬PURINE AND 6-METHYLMERCAPTOPURINE IN PLASMA BY HIGH PERFOR¬MANCE LIQUID CHROMATOGRAPHY-PHOTODIODE ARRAY
ispublished: pub
subjects: RS
abstract: Objective: 6-Mercaptopurin is antineoplastic drug that is included in antimetabolite
group and is used in acute lymphoblastic leukemia medication. 6-Mercaptopurin
is inactive pro-drug that will be metabolized into metabolites. One
of its metabolites is 6-methylmercaptopurine. This study is aimed to optimize
the analytical conditions and perform validation for the analysis of 6-mercaptopurine
and 6-methylmercaptopurine in plasma. Method: Separation was performed
using Waters 2996 HPLC, C18 SunfireTM column (5μm, 250 x 4.6 mm)
with the mobile phase containing water-methanol-acetonitrile with gradient
elution, and detected at 303 nm. 5-Fluorouracil was used as internal standard.
Plasma extraction was done by liquid-liquid extraction using dichloromethane.
Result: The method was linear at concentration range of 2.0 – 200.0 ng/mL
with r > 0.9991 for 6-mercaptopurine and 20 – 2000 ng/mL with r > 0.9993 for
6-methylmercaptopurine. Accuracy and precision within-run and between-run
fulfill the acceptance criteria with % RE and relative standard deviation (%
RSD) ≤ 20% (LLOQ) and ≤ 15% (QC samples). 6-Mercaptopurine and 6-methylmercaptopurine
was stable in plasma at least for 21 days when stored at -20ºC.
Conclusion: The bio-analytical method was sensitive, selective and all the parameters
fulfilled the acceptance criteria of the EMA Bio-analytical Method
Validation Guideline, 2011.
date: 2017
official_url: http://doi.org/10.5530/jyp.2017.1s.8
full_text_status: public
publication: Journal of Young Pharmacists
volume: 9
number: 1
pagerange: s29-s34
refereed: TRUE
issn: 0975-1483
citation:   Supandi, Supandi dan Yahdiana Harahap, Yahdiana dan Nurul Azizah, Nurul dan Rizka Andalusia, Rizka  (2017) SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTO¬PURINE AND 6-METHYLMERCAPTOPURINE IN PLASMA BY HIGH PERFOR¬MANCE LIQUID CHROMATOGRAPHY-PHOTODIODE ARRAY.  Journal of Young Pharmacists, 9 (1).  s29-s34.  ISSN 0975-1483     
document_url: http://repository.uhamka.ac.id/id/eprint/348/1/jurnal%20Young%20Pharm.pdf