TY  - JOUR
VL  - 9
SP  - s29
UR  - http://doi.org/10.5530/jyp.2017.1s.8
EP  - s34
AV  - public
N2  - Objective: 6-Mercaptopurin is antineoplastic drug that is included in antimetabolite
group and is used in acute lymphoblastic leukemia medication. 6-Mercaptopurin
is inactive pro-drug that will be metabolized into metabolites. One
of its metabolites is 6-methylmercaptopurine. This study is aimed to optimize
the analytical conditions and perform validation for the analysis of 6-mercaptopurine
and 6-methylmercaptopurine in plasma. Method: Separation was performed
using Waters 2996 HPLC, C18 SunfireTM column (5?m, 250 x 4.6 mm)
with the mobile phase containing water-methanol-acetonitrile with gradient
elution, and detected at 303 nm. 5-Fluorouracil was used as internal standard.
Plasma extraction was done by liquid-liquid extraction using dichloromethane.
Result: The method was linear at concentration range of 2.0 ? 200.0 ng/mL
with r > 0.9991 for 6-mercaptopurine and 20 ? 2000 ng/mL with r > 0.9993 for
6-methylmercaptopurine. Accuracy and precision within-run and between-run
fulfill the acceptance criteria with % RE and relative standard deviation (%
RSD) ? 20% (LLOQ) and ? 15% (QC samples). 6-Mercaptopurine and 6-methylmercaptopurine
was stable in plasma at least for 21 days when stored at -20ºC.
Conclusion: The bio-analytical method was sensitive, selective and all the parameters
fulfilled the acceptance criteria of the EMA Bio-analytical Method
Validation Guideline, 2011.
A1  - Supandi, Supandi
A1  - Yahdiana Harahap, Yahdiana
A1  - Nurul Azizah, Nurul
A1  - Rizka Andalusia, Rizka
SN  - 0975-1483
IS  - 1
ID  - repository348
TI  - SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTO¬PURINE AND 6-METHYLMERCAPTOPURINE IN PLASMA BY HIGH PERFOR¬MANCE LIQUID CHROMATOGRAPHY-PHOTODIODE ARRAY
Y1  - 2017///
JF  - Journal of Young Pharmacists
ER  -