%0 Journal Article
%@ 0975-1483
%A Supandi, Supandi
%A Yahdiana Harahap, Yahdiana
%A Nurul Azizah, Nurul
%A Rizka Andalusia, Rizka
%A Department of Pharmacy, Faculty of Pharmacy and Science, UHAMKA, Jakarta, Indonesia,
%A Faculty of Pharmacy Universitas Indonesia, Depok, INDONESIA.,
%D 2017
%F repository:348
%J Journal of Young Pharmacists
%N 1
%P s29-s34
%T SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTO¬PURINE AND 6-METHYLMERCAPTOPURINE IN PLASMA BY HIGH PERFOR¬MANCE LIQUID CHROMATOGRAPHY-PHOTODIODE ARRAY
%U http://repository.uhamka.ac.id/id/eprint/348/
%V 9
%X Objective: 6-Mercaptopurin is antineoplastic drug that is included in antimetabolite  group and is used in acute lymphoblastic leukemia medication. 6-Mercaptopurin  is inactive pro-drug that will be metabolized into metabolites. One  of its metabolites is 6-methylmercaptopurine. This study is aimed to optimize  the analytical conditions and perform validation for the analysis of 6-mercaptopurine  and 6-methylmercaptopurine in plasma. Method: Separation was performed  using Waters 2996 HPLC, C18 SunfireTM column (5μm, 250 x 4.6 mm)  with the mobile phase containing water-methanol-acetonitrile with gradient  elution, and detected at 303 nm. 5-Fluorouracil was used as internal standard.  Plasma extraction was done by liquid-liquid extraction using dichloromethane.  Result: The method was linear at concentration range of 2.0 – 200.0 ng/mL  with r > 0.9991 for 6-mercaptopurine and 20 – 2000 ng/mL with r > 0.9993 for  6-methylmercaptopurine. Accuracy and precision within-run and between-run  fulfill the acceptance criteria with % RE and relative standard deviation (%  RSD) ≤ 20% (LLOQ) and ≤ 15% (QC samples). 6-Mercaptopurine and 6-methylmercaptopurine  was stable in plasma at least for 21 days when stored at -20ºC.  Conclusion: The bio-analytical method was sensitive, selective and all the parameters  fulfilled the acceptance criteria of the EMA Bio-analytical Method  Validation Guideline, 2011.