@article{repository348,
          number = {1},
         journal = {Journal of Young Pharmacists},
           title = {SIMULTANEOUS ANALYTICAL METHOD DEVELOPMENT OF 6-MERCAPTO?PURINE AND 6-METHYLMERCAPTOPURINE IN PLASMA BY HIGH PERFOR?MANCE LIQUID CHROMATOGRAPHY-PHOTODIODE ARRAY},
          volume = {9},
           pages = {s29--s34},
            year = {2017},
            issn = {0975-1483},
          author = {Supandi, Supandi and Yahdiana Harahap, Yahdiana and Nurul Azizah, Nurul and Rizka Andalusia, Rizka},
             url = {http://doi.org/10.5530/jyp.2017.1s.8},
        abstract = {Objective: 6-Mercaptopurin is antineoplastic drug that is included in antimetabolite
group and is used in acute lymphoblastic leukemia medication. 6-Mercaptopurin
is inactive pro-drug that will be metabolized into metabolites. One
of its metabolites is 6-methylmercaptopurine. This study is aimed to optimize
the analytical conditions and perform validation for the analysis of 6-mercaptopurine
and 6-methylmercaptopurine in plasma. Method: Separation was performed
using Waters 2996 HPLC, C18 SunfireTM column (5{\ensuremath{\mu}}m, 250 x 4.6 mm)
with the mobile phase containing water-methanol-acetonitrile with gradient
elution, and detected at 303 nm. 5-Fluorouracil was used as internal standard.
Plasma extraction was done by liquid-liquid extraction using dichloromethane.
Result: The method was linear at concentration range of 2.0 - 200.0 ng/mL
with r {\ensuremath{>}} 0.9991 for 6-mercaptopurine and 20 - 2000 ng/mL with r {\ensuremath{>}} 0.9993 for
6-methylmercaptopurine. Accuracy and precision within-run and between-run
fulfill the acceptance criteria with \% RE and relative standard deviation (\%
RSD) {$\leq$} 20\% (LLOQ) and {$\leq$} 15\% (QC samples). 6-Mercaptopurine and 6-methylmercaptopurine
was stable in plasma at least for 21 days when stored at -20?C.
Conclusion: The bio-analytical method was sensitive, selective and all the parameters
fulfilled the acceptance criteria of the EMA Bio-analytical Method
Validation Guideline, 2011.}
}