%V 32
%I Friends Sciences Publisher
%P 71-78
%N 01
%X Inflammation is the body's natural immune response to infection or injury. If the inflammation continues, it will cause organ function failure due to activating inflammatory mediators, including lipoxygenase (LOX). This study aimed to predict the affinity of hydroxychromone and quercetin compounds found in T. indica leaves in inhibiting 5-LOX in silico and In vitro. In silico prediction compares Autodock Tools-1.5.7 software with zileuton. In silico results were used to base In vitro experiments using RAW 264.7 cells induced by lipopolysaccharide. A viability assay was carried out on RAW 264.7 cells using the MTT method. Based on the binding energy and inhibition constant, hydroxychromone and quercetin compounds showed a higher affinity for 5-LOX than zileuton. These results are in line with In vitro research, which shows that ethyl acetate extract of T. indica leaves has a lower LOX activity value (6,763 ± 0.819 mU/mL), which indicates that this extract has potential as an inhibitor of 5-LOX enzyme activity. This extract contained hydroxychromone compounds based on LC-MS/MS analysis from previous research. The viability test shows that a dose of 0-62.5 ppm is safe for RAW 264.7 cells with a viability percentage above 80%. This study can be expanded to hunt for novel natural anti-inflammatory sources that inhibit the 5-LOX enzyme's action.
%A Vera Vera Ladeska
%T artikel IJAB
%D 2024
%L repository34500
%J In Silico and In Vitro Anti-Inflammatory Studies on Lipoxygenase Inhibitor from Tetracera indica Leaves