eprintid: 22047
rev_number: 8
eprint_status: archive
userid: 3858
dir: disk0/00/02/20/47
datestamp: 2023-04-02 10:54:15
lastmod: 2023-04-02 10:54:15
status_changed: 2023-04-02 10:54:15
type: thesis
metadata_visibility: show
creators_name: ANDRIANO, EGO
creators_name: Wahyudi, Priyo
creators_name: Hikmawanti, Ni Putu
title: AKTIVITAS INHIBITOR TIROSINASE METABOLIT KAPANG
ENDOFIT DAUN SUKUN (Artocarpus altilis (Parkinson.) Fosberg
ispublished: pub
subjects: R
subjects: RS
divisions: 48201
abstract: Melanin merupakan zat yang memberi warna cokelat atau cokelat kehitaman pada
kulit. Tirosinase merupakan enzim utama dalam proses biosintesis melanin.
Pemanfaatan daun sukun berdasarkan senyawa kimia yang terkandung
didalamnya seperti flavonoid dan kuersetin yang akan digunakan dalam Uji
aktivitas inhibitor tirosinase. Tujuan penelitian ini adalah untuk mengetahui
aktivitas inhibitors tirosinase oleh metabolit sekunder yang dihasilkan oleh
kapang endofit daun sukun. Metode yang digunakan untuk isolasi kapang endofit
daun sukun adalah metode tanam langsung pada media pertumbuhan Potato
Dextrosa Agar (PDA) dan Potato Dextrose Yeast (PDY) sebagai media kultivasi.
Pengujian aktivitas inhibitor enzim tirosinase dilakukan terhadap supernatan yang
dihasilkan oleh seluruh isolat, hasil pengujian menunjukan supernatan pada DSE I
yang memiliki inhibitor tertinggi, kemudian diekstraksi dan dilakukan pengujian
kembali. Hasil ekstrak kental n-Butanol metabolit kapang endofit daun sukun
isolat DSE I memiliki nilai IC50 261,216 ppm dan ekstrak kental air sebesar
193,642 ppm dengan potensi relatif ekstrak kental n-Butanol 0,220 kali asam
kojat dan ekstrak kental air 0,296 kali asam kojat. Hasil tersebut menunjukan
kedua ekstrak kental air dan butanol belum sebanding dengan asam kojat dalam
menghambat aktivitas tirosinase dan perlu dilakukan penelitian lebih lanjut.
 Kata Kunci: Daun Sukun (Artocarpus altilis (Parkinson.) Fosberg, Kapang
Endofit, Enzim Tirosinase, Inhibitors
date: 2021
date_type: completed
full_text_status: public
institution: Universitas Muhammadiyah Prof. DR. HAMKA
department: Fakultas Farmasi dan Sains
thesis_type: bachelor
thesis_name: bphil
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citation:   ANDRIANO, EGO dan Wahyudi, Priyo dan Hikmawanti, Ni Putu  (2021) AKTIVITAS INHIBITOR TIROSINASE METABOLIT KAPANG ENDOFIT DAUN SUKUN (Artocarpus altilis (Parkinson.) Fosberg.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. HAMKA.   
document_url: http://repository.uhamka.ac.id/id/eprint/22047/1/FS03-220121.pdf