eprintid: 21985
rev_number: 8
eprint_status: archive
userid: 3858
dir: disk0/00/02/19/85
datestamp: 2023-04-02 10:49:01
lastmod: 2023-04-02 10:49:01
status_changed: 2023-04-02 10:49:01
type: thesis
metadata_visibility: show
creators_name: ISTIQOMAH, IIS
creators_name: Wahyudi, Priyo
title: PENENTUAN KONSENTRASI HAMBAT DAN BUNUH MINIMUM
EKSTRAK ETANOL 90% HERBA BARU CINA (Artemisia vulgaris L.)
TERHADAP Candida tropicalis
ispublished: pub
subjects: R
subjects: RS
divisions: 48201
abstract: Infeksi yang disebabkan oleh jamur, umumnya diobati menggunakan pengobatan
sintetik. Penggunaan obat antijamur sintetik secara terus menerus dapat
mengakibatkan resistensi dan efek samping yang serius. Herba baru cina memiliki
berbagai aktivitas farmakologi, salah satunya sebagai antijamur. Tujuan dari
penelitian ini untuk mengetahui konsentrasi hambat dan bunuh minimum ekstrak
etanol 90% herba baru cina terhadap Candida tropicalis. Ekstraksi herba baru cina
dilakukan dengan metode maserasi dengan pelarut etanol 90%, diperoleh rendemen
sebesar 12,44%, kadar abu 2,2702%, dan kadar air 11,0671%. Herba baru cina
mengandung senyawa alkaloid, saponin, tanin, flavonoid, dan terpenoid. Herba baru
cina dibuat dalam beberapa variasi konsentrasi, diujikan terhadap Candida tropicalis,
lalu diamati turbiditas larutan uji serta pemastian hasil dengan penanaman pada
medium Sabouraud Dextrose Agar (SDA) diperoleh nilai KHM dan KBM ekstrak
etanol 90% herba baru cina berturut-turut ialah 18.000 dan 20.000 ppm
Kata kunci: kandidiasis, Candida tropicalis, herba baru cina, konsentrasi hambat dan
bunuh minimum
date: 2022
date_type: completed
full_text_status: public
institution: Universitas Muhammadiyah Prof. DR. HAMKA
department: Fakultas Farmasi dan Sains
thesis_type: bachelor
thesis_name: bphil
referencetext: Aboody MS, Al Mickymaray S. 2020. Anti-fungal efficacy and mechanisms of
flavonoids. Antibiotics, 9(2).1 - 42.
Andrews J. 2001. Determination of minimum inhibitory concentrations. Journal of
Antimicrobial Chemotherapy, Hlm. 5–16.
Apsari AS, Adiguna MS. 2013. Resistensi Antijamur dan Strategi Untuk Mengatasi.
MDVI, 40(2): 1 - 6.
Araujo OE, Flowers FP, King MM. 1990. Griseofulvin: A new look at an old drug.
DICP, Annals of Pharmacotherapy, 24(9): 851–854.
Bangol E, Momuat LI, Abidjulu J. 2014. Aktivitas Antioksidan Ekstrak Etanol dan
N-Heksana dari Daun Rumput Santa Maria (Artemisia vulgaris L.) pada Minyak
Ikan. Jurnal Ilmiah Sains, 14(2):129.
Barney JN, DiTommaso A. 2003. The biology of Canadian weeds. 118. Artemisia
vulgaris L. Canadian Journal of Plant Science, 83(1): 205–215.
Berkow EL, Lockhart SR, Ostrosky-Zeichner L. 2020. Antifungal susceptibility
testing: Current approaches. Clinical Microbiology Reviews, 33(3): 1–30.
Bohlin L. 1998. Natural Products Isolation. Drug Discovery Today, 3(12): 536–537.
Bradley RL. 2007. Moisture and Total Solids Analysis. S.S. Nielsen, ed., Food
Analaysis, 4 ed. New York: Springer, Hlm.94.
Campoy S, Adrio JL. 2017. Antifungals. Biochemical Pharmacology, 133: 86–96.
Canuto MM, Rodero FG. 2002. Antifungal drug resistance to azoles and polyenes.
Lancet Infectious Diseases, 2(9): 550–563.
De Carli L, Larizza L. 1988. Griseofulvin. Mutation Research/Reviews in Genetic
Toxicology, 195(2): 91–126.
Chai LYA, Denning DW, Warn P. 2010. Candida tropicalis in human disease.
Critical Reviews in Microbiology, 36(4): 282–298.
CLSI 2008. Reference Method for Broth Dilution Antifungal Susceptibility Testing
of Yeasts ; Approved Standard — Second Edition Serving the World ’ s Medical
Science Community Through Voluntary Consensus. NCCLS standards and
guidelines. Hlm.1-51.
Cosoveanu A. 2018. Endophytic Fungi in Species of Artemisia. Journal of Fungi,
4(53): 1.
Departemen Kesehatan Republik Indonesia. 2000. Parameter Standar Umum Ekstrak
Tumbuhan Obat. Departemen Kesehatan Republik Indonesia. Jakarta. Hlm.13-
17.
Departemen Kesehatan Republik Indonesia. 1989. Materia Medika Indonesia.
Departemen Kesehatan Republik Indonesia. Jakarta. Hlm. 59.
Ekiert H, Pajor J, Klin P, Rzepiela A, Slesak H, Szopa A. 2020. Significance of
Artemisia vulgaris L. (Common Mugwort) in the history of medicine and its
possible contemporary applications substantiated by phytochemical and
pharmacological studies. Molecules, 25(19): 1–32.
Elgharbawy AA, Zuhanis Y, Hashim Y, Fadwa F, Belgacem B. 2020.
Phytochemicals with antifungal properties: Cure from nature. Malaysian Journal
of Microbiology, 16(4): 1–24.
Endarini LH. 2016. Farmakognisi dan Fitokimia. Pusdik SDM Kesehatan. Jakarta.
hlm132-133, 138.
Fidel PL, Wozniak KL. 2010. Superficial Candidiasis. Topley & Wilson’s
Microbiology and Microbial Infections, Hlm.1–19.
Free SJ. 2013. Fungal Cell Wall Organization and Biosynthesis. 1 ed. Advances in
Genetics, New York: Elsevier Inc. Hlm. 35.
Gajic I, Kabic J, Kekic D, Jovicevic M, Milenkovic M, Mitic Culafic D, Trudic A,
Ranin L, Opavski N. 2022. Antimicrobial Susceptibility Testing: A
Comprehensive Review of Currently Used Methods. Antibiotics, 11(4): 1–26.
Graybill JR, Burgess DS, Hardin, T.C. 1997. Key issues concerning fungistatic
versus fungicidal drugs. European Journal of Clinical Microbiology and
Infectious Diseases, 16(1): 42–50.
Hafsan. 2014. Mikrobiologi Analitik. Alauddin University Press. Makassar. Hlm. 62-
69.
Hanani E. 2014. Analisis Fitokimia. Buku Kedokteran EGC. Jakarta. Hlm.10-11.
Hani U, Shivakumar H, Vaghela RM, Osmani R, Shrivastava A. 2015. Candidiasis: A
Fungal Infection- Current Challenges and Progress in Prevention and Treatment.
Infectious Disorders - Drug Targets, 15(1): 42–52.
Harborne J. 1973. Metode Fitokimia. 2 ed. ITB Press. Bandung. Hlm. 6.
Hidayat R, Hayati, L. 2020. Eureka Herba Indonesia. Eureka Herba Indonesia, 1(1):
1–5.
Hrytsyk RA, Kutsyk RV, Yurchyshyn OI, Struk ОА, Kireev IV, Grytsyk AR. 2021.
The investigation of antimicrobial and antifungal activity of some Artemisia L.
species. Pharmacia, 68(1): 93–100. 
Integrated Taxconomic Information System (ITIS)
https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_va
lue=35505#null. Diakeses pada: 12 Juli 2022, pukul 10.20 WIB
Jiang C, Li Z, Zhang L, Tian Y, Dong D, Peng Y. 2016. Significance of hyphae
formation in virulence of Candida tropicalis and transcriptomic analysis of
hyphal cells. Microbiological Research, 192: 65–72
Jordá T, Puig S. 2020. Regulation of ergosterol biosynthesis in Saccharomyces
cerevisiae. Genes, 11(7): 1–18.
Julianto TS. 2019. Fitokimia Tinjauan Metabolit Sekunder dan Skrining Fitokimia.
Universitas Islam Indonesia. Yogyakarta. Hlm. 18.
Kanafani ZA, Perfect JR. 2008. Resistance to antifungal agents: Mechanisms and
clinical impact. Clinical Infectious Diseases, 46(1): 120–128.
Kementerian Kesehatan Republik Indonesia. 2017. Farmakope Herbal Indonesia.
Edisi II. Kementrian Kesehatan Republik Indonesia. Jakarta. Hlm. 301
Klepser ME, Ernst EJ, Pfaller MA. 1997. Update on antifungal resistance. Trends in
Microbiology, 5(9): 372–375.
Kothavade RJ, Kura MM, Valand AG, Panthaki MH. 2010. Candida tropicalis: Its
prevalence, pathogenicity and increasing resistance to fluconazole. Journal of
Medical Microbiology, 59(8): 873–880.
Kotthoff-Burrell E, Fahy B, Lareau S, Hage C, Sockrider M. 2019. Candidemia
(blood infection) and other candida infections. American Journal of Respiratory
and Critical Care Medicine, 200(5): 9–10.
Kowalska-Krochmal B, Dudek-Wicher R. 2021. The minimum inhibitory
concentration of antibiotics: Methods, interpretation, clinical relevance.
Pathogens, 10(2): 1–21.
Kullberg BJ, Arendrup MC. 2015. Invasive Candidiasis. The New England Journal of
Medicine, Hlm.1445–1456.
Kuper KM, Coyle EA, Wanger A. 2012. Antifungal susceptibility testing: A primer
for clinicians. Pharmacotherapy, 32(12): 1112–1122.
Lee Y, Puumala E, Robbins N, Cowen LE. 2021. Antifungal Drug Resistance:
Molecular Mechanisms in Candida albicans and beyond. Chemical Reviews,
121(6): 3390–3411.
Makanjuola O, Bongomin F, Fayemiwo SA. 2018. An update on the roles of nonalbicans candida species in vulvovaginitis. Journal of Fungi, 4(4). Hlm.4
Meliala L. 2018. Aktivitas Antimutagenik Ekstrak Etanol Herba Binara ( Artemisia
vulgaris L. ) pada Mencit yang Diinduksi Siklofosfamid. Tesis. Fakultas Farmasi
Universitas Sumatera Utara, Medan. Hlm. 42
Perlin DS. 2015. Mechanisms of echinocandin antifungal drug resistance. Annals of
the New York Academy of Sciences, 1354(1):1–11.
Perlin DS, Rautemaa-Richardson R, Alastruey-Izquierdo A. 2017. The global
problem of antifungal resistance: prevalence, mechanisms, and management.
The Lancet Infectious Diseases, 17(12): 383–392.
Pinheiro RR, Singh A, Barreto-Bergter E, Del Poeta M. 2016. Sphingolipids as
targets for treatment of fungal infections. Future Medicinal Chemistry, 8(12):
1469–1484.
Pizzi A. 2021. Tannins medical / pharmacological and related applications: A critical
review. Sustainable Chemistry and Pharmacy. Hlm 1-24.
Rasul MG. 2018. Conventional Extraction Methods Use in Medicinal Plants, their
Advantages and Disadvantages. International Journal of Basic Sciences and
Applied Computing, (6): 10–14.
Robbins N, Wright GD, Cowen LE. 2017. Antifungal drugs: The current
armamentarium and development of new agents. The Fungal Kingdom. Hlm.
903–922.
Rofifah D. 2020. Botani Farmasi. Paper Knowledge. Toward a Media History of
Documents. Universitas Islam Indonesia. Yogyakarta. Hlm. 142-146.
Silva S, Negri M, Henriques M, Oliveira R, Williams DW, Azeredo J. 2012. Candida
glabrata, Candida parapsilosis and Candida tropicalis: Biology, epidemiology,
pathogenicity and antifungal resistance. FEMS Microbiology Reviews, 36(2):
288–305.
Smaill F, Chb MB. 2000. Antibiotic susceptibility and resistance testing: An
overview. Can J Gastroenterol. Hlm. 1-6.
Surain P, Aggarwal NK. 2020. Candida, A Human Pathogen And Major Types Of
Candidiasis. International Journal of Pharmaceutical Sciences and Research,
11(1): 41.
Tamo SPB. 2020. Candida Infections: Clinical Features, Diagnosis and Treatment.
Infectious Diseases and Clinical Microbiology, 2(2): 91–102.
Vincent IR, Carbonari DM, Lewis JD, Forde KA, Goldberg DS, Reddy KR, Haynes
K, Roy JA, Sha D, Marks AR, Schneider JL, Strom BL, Corley DA. 2017. Oral
Azole Antifungal Medications and Risk of Acute Liver Injury, Overall and by
Chronic Liver Disease Status. Am J Med.129(3): 1–23.
White TC, Marr KA, Bowden RA. 1998. Clinical, cellular, and molecular factors that
contribute to antifungal drug resistance. Clinical Microbiology Reviews, 11(2):
382–402.
Wiegand I, Hilpert K, Hancock REW. 2008. Agar and broth dilution methods to
determine the minimal inhibitory concentration (MIC) of antimicrobial
substances. Nature Protocols, 3(2): 163–175.
Witebsky F, MacLowry J, French S. 1979. Broth dilution minimum inhibitory
concentrations: Rationale for use of selected antimicrobial concentrations.
Journal of Clinical Microbiology, 9(5): 589–595.
Xu J. 2021. Is Natural Population of Candida tropicalis Sexual, Parasexual, and/or
Asexual? Frontiers in Cellular and Infection Microbiology, 11: 1–11.
Zhang QW, Lin LG, Ye WC. 2018. Techniques for extraction and isolation of natural
products: A comprehensive review. Chinese Medicine (United Kingdom), 13(1):
1–26.
Zubair Z, Shameem I, Ameen SMN. 2020. a Comprehensive Review With
Pharmacological Potential of “Mother of Herbs”-Artemisia Vulgaris Linn.
World Journal of Pharmacy and Pharmaceutical Sciences, 9: 240–251.
Zuza-Alves, D.L., Silva-Rocha, W.P. & Chaves, G.M. 2017. An update on Candida
tropicalis based on basic and clinical approaches. Frontiers in Microbiology, 8:
1–25.
citation:   ISTIQOMAH, IIS dan Wahyudi, Priyo  (2022) PENENTUAN KONSENTRASI HAMBAT DAN BUNUH MINIMUM EKSTRAK ETANOL 90% HERBA BARU CINA (Artemisia vulgaris L.) TERHADAP Candida tropicalis.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. HAMKA.   
document_url: http://repository.uhamka.ac.id/id/eprint/21985/1/FS03-220466.pdf