eprintid: 21548
rev_number: 8
eprint_status: archive
userid: 3858
dir: disk0/00/02/15/48
datestamp: 2023-04-01 13:35:42
lastmod: 2023-04-01 13:35:42
status_changed: 2023-04-01 13:35:42
type: thesis
metadata_visibility: show
creators_name: Agistina, Nisa
creators_name: Wahyudi, Priyo
creators_name: Yuniarti, Fitri
title: SKRINING POTENSI BAKTERI ENDOFIT RANTING NANGKA
(Artocarpus heterophyllus Lam.) SEBAGAI PENGHASIL ENZIM
XILANASE DAN AMILASE
ispublished: pub
subjects: R
subjects: RS
divisions: 48201
abstract: Bakteri endofit adalah mikroorganisme yang hidup pada jaringan tanaman dengan
waktu tertentu. Ranting nangka (Artocarpus heterophyllus Lam.) salah satu
tanaman berkayu, pohon tahunan yang terdapat mikroba endofit berpotensi
menghasilkan xilanase dan amilase. Tujuan dari penelitian ini untuk mengisolasi
bakteri endofit ranting nangka yang diketahui dapat berpotensi menghasilkan
aktivitas xilanase dan amilase. Isolasi bakteri endofit ranting nangka
menggunakan teknik tanam langsung pada medium NA. Pengujian aktivitas
xilanase dan amilase dilakukan secara kualitatif dilihat dari zona bening yang
terbentuk pada media mengandung xilan dan pati, selanjutnya isolat yang
berpotensi tersebut dikultur dalam fermentasi cair. Metode yang digunakan uji
aktivitas amilase secara kuantitatif dengan Dinitrosalisilat menggunakan
spektrofotometer UV-Vis λ 535. Bakteri endofit yang diperoleh 3 isolat, satu
diantaranya isolat (N3) yang berpotensi menghasilkan amilase. Hasil dari
penelitian ini dapat disimpulkan isolat bakteri endofit yang menghasilkan aktivitas
enzim amilase didapatkan hasil rata-rata yaitu 8,0078 (U/ml). Namun, ketiga
isolat tersebut tidak dapat menghasilkan enzim xilanase.
Kata Kunci: Bakteri Endofit, Ranting Nangka (Artocarpus heterophyllus Lam.),
Xilanase, Amilase.
date: 2021
date_type: completed
full_text_status: public
institution: Universitas Muhammadiyah Prof. DR. HAMKA
department: Fakultas Farmasi dan Sains
thesis_type: bachelor
thesis_name: bphil
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citation:   Agistina, Nisa dan Wahyudi, Priyo dan Yuniarti, Fitri  (2021) SKRINING POTENSI BAKTERI ENDOFIT RANTING NANGKA (Artocarpus heterophyllus Lam.) SEBAGAI PENGHASIL ENZIM XILANASE DAN AMILASE.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. HAMKA.   
document_url: http://repository.uhamka.ac.id/id/eprint/21548/1/FS03-210429.pdf