eprintid: 21547 rev_number: 8 eprint_status: archive userid: 3858 dir: disk0/00/02/15/47 datestamp: 2023-04-01 13:35:39 lastmod: 2023-04-01 13:35:39 status_changed: 2023-04-01 13:35:39 type: thesis metadata_visibility: show creators_name: Vesaliana, Zella creators_name: Wahyudi, Priyo creators_name: Rachmania, Rizky Arcinthya title: SKRINING POTENSI BAKTERI ENDOFIT RANTING KELOR (Moringa oleifera Lam.) SEBAGAI PENGHASIL ENZIM XILANASE DAN AMILASE ispublished: pub subjects: R subjects: RS divisions: 48201 abstract: Ranting kayu kelor memiliki mikroba endofit yang hidup di dalam jaringan tanaman yang dapat menghasilkan enzim ekstraseluler seperti enzim xilanase dan amilase. Tanaman berkayu terdiri dari komponen hemiselulosa dan polimer berbahan dasar karbohidrat, yaitu xilan dan pati. Tujuan penelitian ini untuk memperoleh bakteri endofit ranting kelor yang memiliki potensi tertinggi aktivitas enzim xilanase dan amilase. Bakteri endofit ranting kelor diisolasi menggunakan metode tanam langsung pada medium Nutrient Agar (NA). Pengujian aktivitas enzim secara kualitatif dilakukan dengan mengamati zona bening yang terbentuk di sekitar koloni pada medium xilan dan pati, kemudian dilakukan pengujian kuantitatif dengan menggunakan metode Asam Dinitrosalisilat (DNS). Hasil penelitian isolasi bakteri endofit ranting kelor didapatkan 6 isolat, dua diantara isolat tersebut (KL 1 dan KL 2) menunjukkan kemampuan yang dapat menghasilkan enzim amilase dan isolat KL 2 memiliki potensi tertinggi aktivitas enzim amilase yaitu 8,1939 U/ml. Namun, ke-6 isolat bakteri endofit raning kelor tidak dapat menghasilkan enzim xilanase. Kata Kunci: Ranting Kelor (Moringa oleifera Lam.), Bakteri Endofit, Enzim Xilanase, Enzim Amilase. date: 2021 date_type: completed full_text_status: public institution: Universitas Muhammadiyah Prof. DR. 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