eprintid: 21545
rev_number: 10
eprint_status: archive
userid: 3858
dir: disk0/00/02/15/45
datestamp: 2023-04-01 13:35:34
lastmod: 2023-04-01 13:35:34
status_changed: 2023-04-01 13:35:34
type: thesis
metadata_visibility: show
creators_name: Nurwulandari, Tri
creators_name: Wahyudi, Priyo
creators_name: Yuniarti, Fitri
title: SKRINING POTENSI BAKTERI ENDOFIT RANTING ASAM JAWA
(Tamarindus indica L.) SEBAGAI PENGHASIL ENZIM
XILANASE DAN AMILASE
ispublished: pub
subjects: R
subjects: RS
divisions: 48201
abstract: Bakteri endofit hidup di dalam jaringan tanaman tanpa merugikan tanaman
inangnya, dapat ditemukan pada semua jenis tumbuhan seperti pohon berkayu,
salah satunya pohon asam jawa (Tamarindus indica L.). Penelitian ini bertujuan
untuk skrining bakteri endofit ranting asam jawa yang memiliki aktivitas enzim
xilanase dan amilase yang paling potensial. Isolasi bakteri endofit ranting asam
jawa dilakukan dengan teknik penanaman langsung pada medium Nutrient Agar
(NA). Uji aktivitas enzim secara kualitatif dilakukan menggunakan medium
starch dan xilan dengan mengamati zona bening yang terbentuk di sekitar koloni
bakteri endofit yang ditumbuhkan pada medium, kemudian uji aktivitas enzim
kuantitatif dilakukan menggunakan metode Dinitrosalisilat (DNS) dengan
spektrofotometer UV-Vis λ 535 nm. Isolasi bakteri endofit dari ranting asam
jawa diperoleh 3 isolat yang dua diantaranya (AJ 2, AJ 3) menunjukkan
kemampuan untuk menghasilkan enzim amilase dan isolat AJ 3 memiliki
aktivitas enzim amilase terbesar yaitu 6,3317 U/ml. Namun ketiga isolat tersebut
tidak dapat menghasilkan enzim xilanase.
Kata kunci: Bakteri Endofit, Xilanase, Amilase, Ranting Asam Jawa
(Tamarindus indica L.).
date: 2021
date_type: completed
full_text_status: public
institution: Universitas Muhammadiyah Prof. DR. HAMKA
department: Fakultas Farmasi dan Sains
thesis_type: bachelor
thesis_name: bphil
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citation:   Nurwulandari, Tri dan Wahyudi, Priyo dan Yuniarti, Fitri  (2021) SKRINING POTENSI BAKTERI ENDOFIT RANTING ASAM JAWA (Tamarindus indica L.) SEBAGAI PENGHASIL ENZIM XILANASE DAN AMILASE.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. HAMKA.   
document_url: http://repository.uhamka.ac.id/id/eprint/21545/1/FS03-210426.pdf