eprintid: 21139
rev_number: 8
eprint_status: archive
userid: 3858
dir: disk0/00/02/11/39
datestamp: 2023-02-14 08:21:39
lastmod: 2023-02-14 08:21:39
status_changed: 2023-02-14 08:21:39
type: thesis
metadata_visibility: show
creators_name: Bellaliana, Ria
creators_name: Sunaryo, Hadi
creators_name: Sjahid, Landyyun Rahmawan
title: UJI AKTIVITAS ANTIDEMENSIA NANOEMULSI EKSTRAK LIKOPEN
DARI BUAH SEMANGKA (Citrullus lanatus Thunb.) PADA TIKUS
JANTAN GALUR Sprague dawley DENGAN METODE
MORRIS WATER MAZE
ispublished: pub
subjects: R
subjects: RS
divisions: 48201
abstract: Semangka (Citrullus lanatus Thunb.) mengandung likopen yang tidak larut dalam
air sehingga perlu dibuat dalam bentuk sediaan nanoemulsi. Penelitian ini
bertujuan untuk menguji aktivitas antidemensia dari nanoemulsi ekstrak likopen
dari buah semangka. Penelitian diawali dengan pembuatan ekstrak likopen dengan
metode maserasi dan dilanjutkan dengan pembuatan nanoemulsi ekstrak likopen.
Penelitian ini dibagi enam kelompok yaitu kontrol positif (Donepezil 0,5139
mg/kgBB, kontrol negatif, kontrol normal, dosis 1 (5 mg/kgBB), dosis 2 (10
mg/kgBB), dan dosis 3 (20 mg/kgBB). Semua kelompok menggunakan MWM
bertujuan sebagai navigasi pada hewan percobaan dan diinduksi ECS dengan
intensitas 120mA kecuali kelompok normal, lalu diujikan dengan MWM. Analisa
data dilakukan dengan metode Uji Distribusi Normal dengan melihat ShapiroWilk, Uji Homogenitas, Uji Anova Satu Arah dan Uji Tukey dan hasil pengujian
yang diperoleh berupa %TPPQ. Rara-rata kontrol positif (44,1675%), kontrol
negatif (20%), kontrol normal (35,8325%), dosis 1 (34,1675%), dosis 2 (33,25%),
dosis 3 (45,4175%). Berdasarkan hasil yang didapat dosis 2 (10 mg/kgBB) dan
dosis 1 (5 mg/kgBB) memiliki efek yang sama dengan kontrol normal. Hasil
penelitian menunjukkan semua kelompok dosis uji mempunyai aktivitas anti
demensia karena meningkatkan daya ingat dibandingkan dengan kontrol negatif
(p<0,05). Dosis 3 (20 mg/kgBB) memiliki aktivitas anti demensia yang sebanding
dengan kontrol positif (p>0,05).
Kata Kunci: Nanoemulsi, Antidemensia, Likopen, Morris Water Maze, Buah
Semangka.
date: 2020
date_type: completed
full_text_status: public
institution: Universitas Muhammadiyah Prof. DR. HAMKA
department: Fakultas Farmasi dan Sains
thesis_type: bachelor
thesis_name: bphil
referencetext: Agarwal, S. and Rao, A.V., 2000. Tomato Lycopene And Its Role In Human
Health And Chronic Diseases. International Journal of Canadian Medical
Association or its licensors. Vol 163. No 6. Hlm 740.
Alda, L.M., Gogoasa, I., Bordean, D.M., Gergen, I., Alda, S., Moldovan, C. and
Nita, L., 2009. Lycopene Content Of Tomatoes And Tomato
Products. Journal of Agroalimentary Processes and Technologies. Vol 15. No
4.Hlm 540-542.
Alzheimer’s Association. 2019.Dementiahttp://www.alz.org/alzheimer_s_deme
Ntia. Diperoleh Tanggal 10 Juni 2019. Alzheimer’s Association. 2019.
Anggaeni, D.T. 2017. Uji Aktivitas Antidemensia Jus buah Tomat (Solanum
lycopersicu L.) Berdasarkan Peningkatan Memori Spasial Terhadap Tikus
Model Demensia. Skripsi.. Fakultas Farmasi dan Sains Universitas
Muhammadiyah Prof. DR. HAMKA, Jakarta. Hlm 26.
Ansel, H.C., 1989. Pengantar Bentuk Sediaan Farmasi. Edisi IV. Terjemahan:
Farida Ibrahim. UI Press, Jakarta. Hlm 387-388.
Anton, N. and Vandamme, T.F. 2011. Nano-emulsions and Micro-emulsions:
Clarifications of the Critical Differences. Pharmaceutical Research. Vol 28.
No 5. Hlm 978-985.
Arista, M. 2013. Aktivitas Antioksidan Ekstrak Etanol 80% dan 96% daun katuk
(Sauropus androgynous (L.) Merrr.). Jurnal Ilmiah Mahasiswa Universitas
Surabaya.Vol 2. No 2. Hlm 1-16.
Ariviani, S., Atmaka, W. and Raharjo, S. 2018. Karakterisasi dan Uji Stabilitas
Digestif Nanoemulsi β-Karoten yang Dibuat dengan Metode Emulsifikasi
Spontan. agriTECH. Vol 38. No 1. Hlm 30-38.
Aspamufita, N. dan Yuliani S. 2013. Efek Ekstrak Etanol Rimpang Temulawak
(Curcuma xanthorrhiza Roxb) Terhadap Memori Spasial Tikus Model
Demensia Yang Diinduksi Trimethyltin. Dalam: Pharmaciana. Vol 3. No 2.
Hlm 57-62.
Bala, R., Khanna, D., Mehan, S. and Kalra, S., 2015. Experimental Evidence For
The Potential Of Lycopene In The Management Of Scopolamine Induced
Amnesia. RSC Advances. Vol 5. No 89. Hlm 72881-72892.
Barner, E.L. and Gray, S.L., 1998. Donepezil use in Alzheimer disease. Annals of
Pharmacotherapy. Vol 32 No 1. Hlm 70-77.
Ben, E. S., M. Suardi., T. C. Chalid, dan Tomi. Y. 2013. Optimasi Nanoemulsi
Minyak Kelapa Sawit Menggunakan Sukrosa Monoester. Dalam: Seminar,
Perkembangan Terkini Sains Farmasi dan Klinik III. Hlm 31-62.
Bhatt, P. and Madhav, S., 2011. A Detailed Review On Nanoemulsion Drug
Delivery System. SYNTHESIS. Vol 2. No210. Hlm 2292-2298. 
Bromley-Brits, K., Deng, Y. and Song, W., 2011. Morris Water Maze Test For
Learning And Memory Deficits In Alzheimer's Disease Model Mice. Journal
of Visualized Experiments. Vol 53. Hlm 1-5.
Chauhan, K., Sharma, S., Agarwal, N. and Chauhan, B. 2011. Lycopene of
Tomato Fame: Its Role In Health and Disease. International Journal of
Pharmaceutical Sciences Review and Research, vol 10 no.1. Hlm: 99-115.
Departemen Kesehatan RI. 1995. Farmakope Indonesia. Edisi IV. Badan
Pengawasam Obat dan Makanan, Jakarta. Hlm 6-7, 511, 630, 687, 796.
Departemen Kesehatan RI. 2000. Inventaris Tanaman Obat Indonesia. Jilid I.
Jakarta: Departemen Kesehatan dan Kesejahteraan Sosial RI. Hlm 193-194.
Diba, R.F., Yasni, S. and Yuliani, S., 2014. Nanoemulsifikasi Spontan Ekstrak
Jintan Hitam Dan Karakteristik Produk Enkapsulasinya (Spontaneous
Nanoemulsification of Black Cumin Extract and the Characteristics of
Encapsulation Product). Jurnal Teknologi dan Industri Pangan. Vol 25. No 2.
Hlm 134-139.
Duong, S., Patel, T. and Chang, F., 2017. Dementia: What Pharmacists Need To
Know. Canadian Pharmacists Journal/Revue des Pharmaciens du Canada.
Vol 150. No 2. Hlm 118-129.
Elvira., Sylvia, D., Hadisukanto. dan Gitayanti. 2010. Buku Ajar Psikiatri. Badan
Penerbit Fakultas Kedokteran Universitas Indonesia, Jakarta. Hlm 197-208.
Gadhave, A.D., 2014. Nanoemulsions: Formation, Stability and Application.
International Journal for Research in Sciesce and Advanced Technologies.
Vol 2. No 3. Hlm 38-43.
Gupta, A., Eral, H. B., Hatton, T. A. and Doyle, P. S. 2016. Nanoemulsions
Formation, Properties and Application. The Royal Society of Chemistry. Vol
12. No 11. Hlm : 2826-2841.
Gupta, P.K., Pandit, J.K., Kumar, A., Swaroop, P. and Gupta, S., 2010.
Pharmaceutical Nanotechnology Novel Nanoemulsion-high Energy
Emulsification Preparation, Evaluation and Application. The Pharma
Research. Vol 3. Hlm 117-138.
Ha, T.V.A., Kim, S., Choi, Y., Kwak, H.S., Lee, S.J., Wen, J., Oey, I. and Ko, S.
2015. Antioxidant Activity and Bioaccessibility of Size-different
Nanoemulsions For Lycopene-enriched Tomato Extract. Food Chemistry. Vol
178. Hlm 115-121.
Halnor, V.V., Pande, V.V., Borawake, D.D. and Nagare, H.S. 2018.
Nanoemulsion: A Novel Platform For Drug Delivery System. J Mat Sci
Nanotechol, vol 6 no.1, hlm:104.
Hanani, E. 2015. Analisis Fitokimia. Jakarta: Buku Kedokteran EGC. Hlm 79-90.
Handayani, S.F., Nugroho, H.B., dan Munawiroh, Z.S. 2018. Optimasi Formulasi
Nanoemulsi Minyak Biji Anggur Energi Rendah dengan D-Optimal Mixture
Design (DMD). Jurnal Ilmiah Farmasi, vol 14 no. 1. Hlm: 20. 
Harbone, JB. 1987. Metode Fitokimia Penuntun Cara Modern Menganalisa
Tumbuhan. ITB. Bandung.
Haroon, S. 2014. Extraction of Lycopene from Tomato Paste and its
Immobilization for Controlled Release. Thesis. The University of WAIKATO.
Hlm 50.
Hodisan, T., Socaciu, C., Ropan, I. and Neamtu, G., 1997. Carotenoid
Composition of Rosa Canina Fruits Determined By Thin Layer
Chromatography and High Performance Liquid Chromatography. Journal of
Pharmaceutical and Biomedical Analysis. Vol 16. No 3. Hlm 521-528.
Huang, S. and Chang, W.H., 2009. Advantages of Nanotechnology-based Chinese
Herb Drugs On Biological Activities. Current Drug Metabolism. Vol 10. No
8. Hlm 905-913.
ITIS report: Citrullus lanatus (Thumb). Matsum & Nakai Taxonomic Serial No
527396 Dalam https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TS
_topic=TSN&search_value=527396#nullDiperoleh Tanggal 29 maret 2019.
Jaiswal, M., Dudhe, R. and Sharma, P.K., 2015. Nanoemulsion: An Advanced
Mode Of Drug Delivery System. 3 Biotech. Vol 5. No 2. Hlm 123-125.
Jelic, V. and Darreh-Shori, T., 2010. Donepezil: a review of pharmacological
characteristics and role in the management of Alzheimer disease. Clinical
Medicine Insights: Therapeutics. Vol 2 No 1. Hlm: 772.
KEMENKES RI. 2008. Farmakope Herbal Indonesia. Edisi I. Kementerian
Kesehatan Republik Indonesia, Jakarta. Hlm 165.
KEMENKES RI. 2010. Farmakope Herbal Indonesia. Edisi I. Kementerian
Kesehatan Republik Indonesia, Jakarta. Hlm 136.
Kim, G.H., Kim, J.E., Rhie, S.J. and Yoon, S., 2015. The Role Of Oxidative
Stress In Neurodegenerative Diseases. Experimental Neurobiology. Vol 24.
No 4. Hlm 325-335.
Kumar, N. V., Elango, P., Asmathulla, S., and Kavimani, S. 2017. A Systematic
Review on Lycopene and its Beneficial Effects”. Biomedical and
Pharmacology Journal, Vol.10 No.4. Hlm: 2115.
Kusumastuti, U.D., Sukarsa, S. and Widodo, P., 2017. Keanekaragaman Kultivar
Semangka [Citrullus Lanatus (Thunb.) Matsum. & Nakai] di Sentra Semangka
Nusawungu Cilacap. Scripta Biologica. Vol 4. No 1. Hlm 15-19.
Launer, L.J., Andersen, K., Dewey, M., Letenneur, L., Ott, A., Amaducci, L.A.,
Brayne, C., Copeland, J.R.M., Dartigues, J.F., Kragh-Sorensen, P. and Lobo,
A., 1999. Rates and Risk Factors For Dementia and Alzheimer’s Disease:
Results from EURODEM Pooled Analyses. Neurology. Vol 52 No 1. Hlm
1,52,78-84.
Luo, J., Min, S., Wei, K., Cao, J., Wang, B., Li, P., Dong, J. and Liu, Y., 2014.
Propofol Prevents Electroconvulsive-shock-induced Memory Impairment
Through Regulation Of Hippocampal Synaptic Plasticity In a Rat Model of
Depression. Neuropsychiatric Disease and Treatment. Vol 10. Hlm 1847-
1859.
Luo, J., Min, S., Wei, K., Li, P., Dong, J. and Liu, Y.F., 2011. Propofol Protects
Against Impairment of Learning-memory and Imbalance of Hippocampal
Glu/GABA Induced by Electroconvulsive Shock in Depressed Rats. Journal
of anesthesia. Vol 25. No 5. Hlm 657-665.
Martin A, Swarbick J. and Cammarata A. 1993. Farmasi Fisik II Edisi 3.
Terjemahan: Yoshita. UI Press, Jakarta. Hlm 1077-1160.
Miller, L.A., Hsieh, S., Lah, S., Savage, S., Hodges, J.R. and Piguet, O., 2012.
One Size Does Not Fit All: Face Emotion Processing Impairments In
Semantic Dementia, Behavioural-variant Frontotemporal Dementia and
Alzheimer's Disease Are Mediated by Distinct Cognitive
Deficits. Behavioural Neurology. Vol 25. No 1. Hlm 53-58.
Monica, E. and Rollando, R., 2019. Identifikasi dan Isolasi Senyawa Likopen dari
Semangka (Citrullus Lanatus).Jurnal Ilmu Farmasi dan Farmasi Klinik. Vol.
16. No.01. Hlm 80-85.
Naz, A., Butt, M.S., Sultan, M.T., Qayyum, M.M.N. and Niaz, R.S., 2014.
Watermelon Lycopene and Allied Health Claims. EXCLI journal. Vol 13.
Hlm 255-260.
Ningrum, R., 2015. Identifikasi Senyawa Alkaloid dari Batang Karamunting
(Rhodomyrtus tomentosa) Sebagai Bahan Ajar Biologi Untuk SMA Kelas X.
Skripsi. University of Muhammadiyah Malang. Hlm 1-8.
Novita, M., Mangimbulude, J. and Rondonuwu, F.S. 2010. Prosiding Seminar
Nasional Sains dan Pendidikan Sains Universitas Kristen Satya Wacana.Hlm
30-31.
Nunez, J. 2008. Morris Watrer Maze Experiment. Journal of Visualized
Experiments. Vol 19. Hlm 1-2.
Pahuja, M., J. Mehla and Y.K. Gupta. 2012. Anticonvulsant and Antioxidative of
Hydroalcoholic Extract of Tuber of Orchis Mascula in Pentylenetetrazole and
Maximal Electroshock Induced Seizure in Rats. J Ethnopharmacol. Vol. 142.
No 1. Hlm 23-27.
Phaniendra, A., Jestadi, D.B. and Periyasamy, L., 2015. Free Radicals: Properties,
Sources, Targets, and Their Implication in Various Diseases. Indian Journal
of Clinical Biochemistry. Vol 30. No 1. Hlm 11-12.
Purnamaningsih, H., Nururrozi, A. and Indarjulianto, S., 2017. Saponin: Dampak
terhadap Ternak (Ulasan). Jurnal Peternakan Sriwijaya. Vol 6. No 2. Hlm 79-
80.
Reagen-Shaw, S., Nihal, M. and Ahmad, N. 2008. Dose Translation From Animal
To Human Studies Revisited. The FASEB journal. Vol 22. No 3. Hlm 659-
661.
Robinson, T. 1995. Kandungan organik tumbuhan tinggi. Bandung: ITB. Vol 14
No.33. Hlm 1-6.
Rodriguez-Amaya, D.B., 2001. A guide to carotenoid analysis in foods.
Washington: ILSI press. Hlm 5-10.
Rusdi, M. 2013. Desain Formulasi Nanoemulsi Pewarna Alam Ekstrak Kayu
Secang (Caesalpinia sappan linn). Tesis. Magister Sains Program Studi
Teknologi Industri Pertanian IPB, Bogor. Hlm 29,32.33.
Safwan S, Yuliani S. dan Pramono S. 2014. Uji Aktivitas Minyak Atsiri Rimpang
Kunyit (Curcuma longa Linn) Pada Tikus Sprague Dawley Model Demensia.
Dalam: Kartika Jurnal Ilmiah Farmasi. Hlm 20-26.
Salamah, N., Rozak, M., Al Abror, Muhti. 2017. Pengaruh Metode Penyarian
Kadar Alkaloid Total Daun Jembirit dengan Metode Spektrofotometri Visibel.
Pharmaciana. Vol 7. No 1. Hlm 113-122.
Salvia-Trujillo, L., Martin-Belloso, O. and McClements, D., 2016. Excipient
Nanoemulsions For Improving Oral Bioavailability Of Bioactives.
Nanomaterials. Vol 6. No 1. Hlm 17.
Sari, A.I., Herdiana, Y. 2018. Formulasi Nanoemulsi Terhadap Peningkatan
Kualitas Obat. Farmaka. Vol 16. No 1. Hlm 247-252.
Sari, D. K, dan R. S. D. Lestari. 2015. Pengaruh Waktu Dan Kecepatan
Pengadukan Terhadap Emulsi Minyak Biji Matahari (Helianthus anuus L)
Dan Air. Dalam: Jurnal Integrasi Proses. Vol 5. No 3. Hlm 312-322.
Sari, D.C.R., Pratama, R.S., Aswin, S. and Suharmi, S., 2016. Pengaruh Durasi
Pemberian Ekstrak Etanol Pegagan (Centella asiatica sp.) terhadap Memori
Spasial Tikus Putih (Rattus norvegicus) pascastres Listrik. Mutiara Medika:
Jurnal Kedokteran dan Kesehatan. Vol 12. No 2. Hlm 67-68.
Savardekar, P., and A. Bajaj. 2016. Nanoemulsions. Dalam :International of
Research In Pharmacy and Chemistr. Vol 06. No 2. Hlm 312-322.
Shi, J., and Le Maguer. M. 2000. Lycopene In Tomatoes: Chemical And Physical
Properties Affected By Food Processing. Dalam: Critical Reviews in
Biotechnology. Vol 20. No 4. Hlm 293-334.
Singh, R., Lillard, JW. 2009. Review Nanoparticle Based Targeted Drug
Delevery. Experimental and Molecular Pathology. Hlm 86, 215-223.
Singh, R.P., Sharad, S. and Kapur, S., 2004. Free Radicals and Oxidative Stress in
Neurodegenerative Diseases: Relevance of Dietary Antioxidants. J Indian
Acad Clin Med. Vol 5. No 3. Hlm 218-225.
Sinha, N., Dua, D. and Wadhwa, D. 2015. Antioxidant Activity, Free Radical
Scavanging Capacity and Phytochemical Screening of Some Selected Indian
Plants of Medicinal Importance. Int J Innov Pharm Sci Res. Vol 3. Hlm 151-
63. 
Sulastri, E., Ikram, M. and Yuliet, Y., 2017. Uji Stabilitas Dan Aktivitas
Antioksidan Mikroemulsi Likopen Tomat (Solanum lycopersicum L.). Jurnal
Farmasi Galenika (Galenika Journal of Pharmacy). Vol 3 No 1. Hlm: 15.
Suryadi, D. 2017. Uji Aktivitas Jus Kukusan Buah Semangka (Citrullus lanatus
thumb.) Terhadap Peningkatan Memori Spasial Pada Tikus Model Demensia.
Skripsi.. Fakultas Farmasi dan Sains Universitas Muhammadiyah Prof. DR.
HAMKA, Jakarta. Hlm 18.
Swain, S., C. N. Patra, and Bhanoji, R. M. E. 2016. Pharmaceutical Drug
Delivery System and Vehicles. Woodhed Publishing India. Hal. 27, 31-32,38.
Syafitri, S. 2018. Pengaruh Kecepatan Pengadukan Terhadap Sifat Fisik
Nanoemulsi Ekstrak Likopen Buah Semangka (Citrullus lanatus). Skripsi.
Fakultas Farmasi dan Sains Universitas Muhammadiyah Prof. DR. HAMKA,
Jakarta. Hlm 1-34.
Tadmor, Y., King, S., Levi, A., Davis, A., Meir, A., Wasserman, B., Hirschberg,
J. and Lewinsohn, E., 2005. Comparative Fruit Colouration in Watermelon
and Tomato. Food Research International. Vol 38. No 8. Hlm 837-841.
United States Department of Agriculture. 2019. Agricultural Research Service.
Dalam https://ndb.nal.usda.gov/ndb/foods/show/09326?fgcd=&manu=&form
at=&count=&max=25&offset=&sort=default&order=asc&qlookup=watermelon&
ds=&qt=&qp=&qa=&qn=&q=&ing=. Diperoleh tanggal 24 Juni 2019.
Uttara, B., Singh, A.V., Zamboni, P. and Mahajan, R.T., 2009. Oxidative Stress
and Neurodegenerative Diseases: a Review of Upstream and Downstream
Antioxidant Therapeutic Options. Current Neuropharmacology. Vol 7. No 1.
Hlm 65-74.
Vorhees, C.V. and Williams, M.T., 2006. Morris Water Maze: Procedures For
Assessing Spatial and Related Forms of Learning and Memory. Nature
Protocols. Vol 1. No 2. Hlm 848-858.
Yuliani S. 2016. Efek Ekstrak Rimpang Kunyit (Curcuma longa Linn) Terstandar
Kurkumin. Tikus Model Demensia Yang Diinduksi Trimetiltin. Disertasi.
Fakultas Kedokteran Universitas Gadjah Mada, Yogyakarta. Hlm 1-170.
citation:   Bellaliana, Ria dan Sunaryo, Hadi dan Sjahid, Landyyun Rahmawan  (2020) UJI AKTIVITAS ANTIDEMENSIA NANOEMULSI EKSTRAK LIKOPEN DARI BUAH SEMANGKA (Citrullus lanatus Thunb.) PADA TIKUS JANTAN GALUR Sprague dawley DENGAN METODE MORRIS WATER MAZE.  Bachelor thesis, Universitas Muhammadiyah Prof. DR. HAMKA.   
document_url: http://repository.uhamka.ac.id/id/eprint/21139/1/FS03-210092.pdf