eprintid: 20960
rev_number: 9
eprint_status: archive
userid: 3858
dir: disk0/00/02/09/60
datestamp: 2023-02-13 02:50:06
lastmod: 2023-02-13 02:50:06
status_changed: 2023-02-13 02:50:06
type: thesis
metadata_visibility: show
creators_name: Rafiq, Muhammad
creators_name: Hikmawanti, Ni Putu Ermi
creators_name: Fatmawati, Sofia
title: KADAR TOTAL FENOLIK DAN PENGUJIAN TOKSISITAS AWAL
DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)
PADA EKSTRAK ETANOL 70 % TANAMAN GALING (Causonis
trifolia (L.) Mabb. & J.Wen)
ispublished: pub
subjects: R
subjects: RS
divisions: 48201
abstract: Tanaman Galing (Causonis trifolia (L.) Mabb. & J.Wen.) adalah salah satu tanaman
yang banyak digunakan sebagai pengobatan di Indonesia. Pada tanaman galing
memiliki senyawa turunan fenol yang dapat berpotensi menghambat aktivitas untuk
menurunkan aktivitas dari sel kanker, seperti reveratrol, viniferin, ampelosin, dan
piceid. Dalam memperoleh kualitas kandungan dalam ekstrak dipengaruhi beberapa
faktor, salah satunya metode ekstraksi. Tujuan penelitian ini adalah untuk
mengetahui pengaruh variasi metode ekstraksi (Maserasi, Refluks, dan Ultrasound
Assisted Extraction (UAE)) pada tanaman galing terhadap perolehan kandungan
fenolik total dan efek toksisitas terhadap Artemia salina dengan metode BSLT.
Pada penentuan total fenolik, digunakan asam galat sebagai pembaku dengan
menggunakan Spektrofotometer UV-Vis dengan hasil akhir adalah mgGAE/g
sampel. Hasil total fenolik Refluks yaitu 867,2131 mgGAE/g, Maserasi 754,8894
mg GAE/g dan UAE 718,5983 mgGAE/g. Pada toksisitas menggunakan BSLT,
parameter efek toksisitas yang dapat diukur dengan menggunakan nilai Letal
Consentration 50 (LC50). Hasil penelitian ini didapatkan LC50 pada metode Refluks
sebesar 2559,553 µg/ml, Maserasi sebesar 2645,534 µg/ml, dan UAE sebesar
2883,715 µg/ml. Total fenolik tertinggi diperoleh dari metode ekstraksi refluks.
Berdasarkan hasil uji sitotoksik dengan metode BSLT dapat disimpulkan bahwa
ekstrak etanol 70% tanaman galing memiliki efek sitotoksik yang lemah.
Kata kunci : Aktivitas Toksiksitas, Brine Shrimp Lethality Test, Kadar Fenolik
Total, Tanaman Galing
date: 2021
date_type: completed
full_text_status: public
institution: Universitas Muhammadiyah Prof. DR. HAMKA
department: Fakultas Farmasi dan Sains
thesis_type: bachelor
thesis_name: bphil
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citation:   Rafiq, Muhammad dan Hikmawanti, Ni Putu Ermi dan Fatmawati, Sofia  (2021) KADAR TOTAL FENOLIK DAN PENGUJIAN TOKSISITAS AWAL DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) PADA EKSTRAK ETANOL 70 % TANAMAN GALING (Causonis trifolia (L.) Mabb. & J.Wen).  Bachelor thesis, Universitas Muhammadiyah Prof. DR. HAMKA.   
document_url: http://repository.uhamka.ac.id/id/eprint/20960/1/FS03-210273.pdf