eprintid: 17997 rev_number: 9 eprint_status: archive userid: 1993 dir: disk0/00/01/79/97 datestamp: 2023-02-01 09:35:04 lastmod: 2023-02-01 09:35:04 status_changed: 2023-02-01 09:35:04 type: article metadata_visibility: show creators_name: Budiman, Muhamad Arif creators_id: arifbudiman@uhamka.ac.id title: Aktivitas Antioksidan, Total Fenolik, dan Total Flavonoid Madu Apis mellifera dari Hutan Akasia (Accacia crassicarpa) Riau, Indonesia dengan Beberapa Perlakuan Pengeringan ispublished: pub subjects: QD subjects: QL abstract: Madu Indonesia memiliki kadar air yang cukup tinggi dan seringkali melebihi batas maksimal standar kualitas madu berdasarkan regulasi nasional dan internasional. Hal ini disebabkan oleh kelembaban udara yang cukup tinggi dan karakter higroskopis madu. Provinsi Riau merupakan salah satu penghasil madu tertinggi di Indonesia, namun kadar airnya cukup tinggi yaitu pada rentang 23-27%. Salah satu upaya untuk mengurangi kadar air madu adalah dengan pemanasan, namun senyawa metabolit sekunder pada madu berisiko mengalami kerusakan dan berdampak pada penurunan sifat fungsional madu terhadap kesehatan. Penelitian terkait pengaruh pemanasan terhadap stabilitas senyawa metabolit sekunder pada madu belum banyak dilakukan. Penelitian ini bertujuan untuk melihat pengaruh pemanasan terhadap stabilitas senyawa metabolit sekunder pada madu Apis mellifera hutan akasia dari Provinsi Riau, Indonesia, diantaranya analisis kadar air, penapisan senyawa metabolit sekunder, pengukuran aktivitas antioksidan, pengukuran TPC dan pengukuran TFC. Hasil penelitian menunjukkan bahwa perlakuan panas 50oC selama 5 hari dan perlakuan panas 105oC selama 6 jam dapat menurunkan kadar air madu tanpa mengganggu stabilitas senyawa metabolit sekunder pada madu. Hal ini ditunjukkan pada nilai IC50 madu tanpa perlakuan (21103,74 μg/mL) yang lebih besar dari madu dengan perlakuan 50oC (16503,83 μg/mL) dan 105oC (777,33 μg/mL). Nilai IC50 yang rendah menunjukkan aktivitas antioksidan yang tinggi, sejalan dengan TPC dan TFC. Nilai TPC dan TFC sampel tanpa perlakuan (TPC= 0,67 mgGAE/g; TFC=0,34 mgQE/g) lebih rendah dari sampel dengan pemanasan 50oC (TPC=1,59 mgGAE/g; TFC=0,46 mgQE/g) dan lebih rendah dari sampel dengan pemanasan 105oC (TPC=13,94 mgGAE/g; TFC=4,37 mgQE/g)." date: 2022 date_type: published publisher: Pusat Penelitian Biologi LIPI official_url: https://jurnalbiologi.perbiol.or.id/home/article/58eab3a6-f608-4235-bf96-47c86fce4cc0 id_number: 10.47349/jbi/18022022/231 full_text_status: public publication: Jurnal Biologi Indonesia volume: 18 number: 2 pagerange: 231-243 refereed: TRUE issn: 2338-834X referencetext: [BSN] Badan Standar Nasional. 1992. SNI 01-2891-1992. Cara Uji Makanan dan Minuman. Badan Standarisasi Nasional. 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