<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell\r\nline: A model system to understand Type 2 Diabetes Mellitus"^^ . "Type 2 Diabetes Mellitus (T2DM) is one of the major health issues in the world. The cellular mechanism of T2DM is still not fully understood.\r\nIt could be studied by using spheroid three-dimensional (3D) culture which is considered representative of the in vivo conditions. Several types\r\nof pancreatic β cell lines have been used, one of which is the insulin-GLase (iGL) cell line. This study aims to evaluate the effect of cell density\r\nand incubation time on spheroid morphology and cell viability in order to understand which one can be considered as the best option in studying\r\nT2DM using iGL cell. Spheroid was made by using the Hanging drop method. The variations of initial seeding cells were 50, 100, 200, and 400\r\ncells/µL then incubated for 1, 2, 3, and 4 days. The evaluated parameters in this study are spheroid morphology and cell viability. Spheroid\r\nmorphology was observed by using inverted phase contrast microscope integrated with camera (Nikon) and NIS-Elements Analysis D software.\r\nCell viability was determined by using LUNA-II™ Automated Cell Counter (Logos Biosystem). The result of this study showed that spheroid in\r\nall of the group cell concentration have formed since the first day and its diameter was significantly increased on the following days (p<0,05).\r\nThe spheroid size was positively correlated with the cell density in group 50-200 cells/µL. A single and stable spheroid morphology was\r\nobserved in 50-100 cells/µL group. Cell viability in 3D culture system was lower and significantly decreased since day 3 compared to 2D culture\r\n(p <0.05; 0.01). In conclusion, spheroid derived from iGL cell line with a stable morphology and good viability could be obtained from a cell\r\nconcentration of 50-100 cells / µL with two days of incubation.\r\nKeywords: iGL, cell density, spheroid, viability"^^ . "2021-04-23" . . "38" . "3" . . "Journal of Experimental and Clinical Medicine"^^ . . . "Journal of Experimental and Clinical Medicine"^^ . . . . . . . . . . . . . . . . . . . . . . . "Wawang"^^ . "Wawang Setiawan Sukarya"^^ . "Wawang Wawang Setiawan Sukarya"^^ . . "Yashiro"^^ . "Takashi Yashiro"^^ . "Yashiro Takashi Yashiro"^^ . . "Endin"^^ . "Endin Nokik Stujanna"^^ . "Endin Endin Nokik Stujanna"^^ . . "Suci"^^ . "Sri Suciati Ningsih"^^ . "Suci Sri Suciati Ningsih"^^ . . "Erlin"^^ . "Erlin Listyaningsih"^^ . "Erlin Erlin Listyaningsih"^^ . . "Kiki"^^ . "Rizkyana Avissa"^^ . "Kiki Rizkyana Avissa"^^ . . . . . . "Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell\r\nline: A model system to understand Type 2 Diabetes Mellitus (Text)"^^ . . . . . "Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell line_ A model system to understand Type 2 Diabetes Mellitus.pdf"^^ . . . "Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell\r\nline: A model system to understand Type 2 Diabetes Mellitus (Other)"^^ . . . . . . "Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell\r\nline: A model system to understand Type 2 Diabetes Mellitus (Other)"^^ . . . . . . "Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell\r\nline: A model system to understand Type 2 Diabetes Mellitus (Other)"^^ . . . . . . "Evaluation of morphology and viability of spheroid derived from Insulin-GLase cell\r\nline: A model system to understand Type 2 Diabetes Mellitus (Other)"^^ . . . . . "HTML Summary of #17282 \n\nEvaluation of morphology and viability of spheroid derived from Insulin-GLase cell \nline: A model system to understand Type 2 Diabetes Mellitus\n\n" . "text/html" . . . "R Medicine"@en . .