eprintid: 17168
rev_number: 16
eprint_status: archive
userid: 1940
dir: disk0/00/01/71/68
datestamp: 2022-10-09 07:15:28
lastmod: 2022-10-09 07:15:28
status_changed: 2022-10-09 07:15:28
type: thesis
metadata_visibility: show
creators_name: Rini Prastiwi, Rini
creators_id: rini_prastiwi@uhamka.ac.id
title: FEK HEPATOPROTEKTOR DAN IMUNOMODULATOR
BATANG BROTOWALI (Tinospora cordifolia Miers) PADA
INDUKSI DENGAN VAKSIN HEPATITIS B
ispublished: unpub
subjects: QH301
subjects: QK
subjects: R
subjects: RM
divisions: 48201
abstract: Tujuan dari penelititan ini adalah untuk mengevaluasi kemampuan brotowali
terhadap pengaturan fungsi hepatoprotektif dan imunomodulator dengan penginduksi
vaksin hepatitis B pada mencit balb c.
Pengujian aktifitas imunomodulator dengan menggunkan daya fagositosis sel
makrofag, sedangkan pengujian hepatoprotektor dengan pemeriksaaan serum ALT
(Alanin Aminotransaminase) dan perubahan histopatologi sel hati. Hewan uji dibagi
menjadi 6 kelompok perlakuan. Masing-masing terdiri dari 5 mencit. Kelompok T1
diberikan fraksi kloroform metanol dosis 0,3416 mg/20 gram BB mencit, T2 0,4844
mg/20 gram BB mencit, T3 0,0942 mg/20 gram BB mencit, T4 0,8671 mg/20 gram BB
mencit), kelompok kontrol positif dan kelompok kontrol negatif. Induksi menggunakan
vaksin hepatitis B (0,52 mcg / 20 g BB mencit) pada hari ke 7 dan 14. Percobaan
dilakukan selama 28 hari.
Hasil penelitian menunjukkan bahwa pengobatan dengan fraksi kloroform :
metanol Tinospora cordifolia Miers memberikan pertolongan pada perbaikan fungsi hati
dan fungsi imun pada penginduksian dengan vaksin hepatitis B. Fraksi yang aktif
sebagai imunostimulan dan hepatoprotektor adalah fraksi T1 dengan dosis 0,3416 mg/20
gram BB mencit.
date: 2009-10-17
date_type: submitted
full_text_status: public
pages: 82
institution: USB
department: Farmasi
thesis_type: masters
thesis_name: other
referencetext: Anonim, 2005, Hepatitis B Vaccine, di akses dari http://www.cdc.gov.hip/publication/vip
hep-b.pdf, diakses 5 Mei 2008-05-15
Anonim, 1978, Dyeing Reagents for Thin Layer and Paper Chromatography, p.41,
E.Merck, Darmstadt, Germany.
Adhavaryu et al., 2007, Effect of Four Indian Medicinal Herbs on Isoniazid, Rifampicin,
and Pyrazinamid, induced Hepatic Injury and Imunosupression in Guinea Pigs,
India
Angel JH, Sommer AR, Citron MK., 1979, Toxicity of antituberculosis drug with special
reference to hepatotoxicity. Bull IUAT 1979; 54(47).
Benjamini, E., Coic R., Sunshine., 2000, Imunology, Ashort Course, Edisi IV, A. John
Willey & Suns, Inc, Publication, New York.
Burgess G.W, 1994, Prinsip Dasar Elisa, dan Variasi Konvigurasi, dalam Burgesss, B.W.,
ELISA, dalam Diagnosis dan Penelitian, Diterjemahkan oleh Wayan T., Adana,
Gajah Mada University, Yogyakarta.
Bairy , 2004, Efficacy of Tinospora cordifolia on Learning and Memory in Healthy
Volunteers: A Double-Blind, Randomized, Placebo Controlled Study, 1735-
2657/04/32-57-60, Iranian Journal Of Pharmacology & Therapeutic .
Bairy dan karkal, 2007, Razi Institute for Drug Research (RIDR) , Safety of Aqueous
Extract of Tinospora cordifolia (Tc) in Healthy Volunteers: A Double Blind
Randomised Placebo Controlled Study, Iranian Journal of Pharmacology &
Therapeutics, IJPT 6:59-61.
Baron, D.N Kapita Selekta Patologi Klinik, 1981, diterjemahkan oleh Andrianto dan
Gunawan, Penerbit Buku Kedokteran ,Jakarta, 1981
Bishayi et al.., 2002, Turki, Hepatoprotective an modulatory Properties of Tinospora
cordifolia In CCL4 Intoxicated Mature Albino Rats, The Journal Toxicology
Sciences, Vol 27, No. 3, 139-146
Chandrasoma dan Taylor. 2006. Ringkasan Patologi Anatomi. Edisi 2. Jakarta : EGC
Chintalwar G, Jain A, Sipahimalani A, Banerji A, Sumariwalla P, Ramakrishnan R, et al.,
1999, An immunologically active arabinogalactan from Tinospora cordifolia.
Phytochemistry. 1999;52:1089–93.
56
Desai, 2002, An immunomodulator from Tinospora cordifolia with antioxidant activity in
cell-free systems, Proc. Indian Acad. Sci. (Chem. Sci.), Vol. 114, No. 6,
December 2002, pp 713–719
Dahanukar SA, Thatte UM, Pai NR, More PB, 1988, Karandikar SM.
Immunotherapeutic modification by Tinospora cordifolia of abdominal sepsis
induced by caecal ligation in rats. Ind J Gastroenterol. 1988;7:21–3.
Dahanukar et al., 2000, Pharmacology Of Medicinal Plants And Natural Products, Indian
Journal Of Pharmacology,vol 32, Mumbai, 81-118
Dhanasekaran et al., 2008, Chemopreventive potential of Epoxy clerodane diterpene
from Tinospora cordifolia against diethylnitrosamine-induced hepatocellular
carcinoma., 14 Oktober 2008.
DepKes RI., 2001, Inventaris Tanaman Obat (I), jilid 2, Departemen Kesehatan dan
Kesejahteraan Sosial RI, Badan Penelitian dan Pengembangan Kesehatan, 337
Gibbs, 2000, Anticancer Drug Target : Growth Factorand Growth Factor Signaling,
J.Clin, Inves, 105 (1)
Gangan VD, Pradhan P, Sipahimalani AT., 1997, Phytoecdysones from Tinospora
cordifolia: structural elucidation of ecdysterone and makisterone A by 2D NMR
spectroscopy. Ind J Chem. 1997;36B:787–92.
Gregory A. Poland, M.D., and Robert M. Jacobson, M.D, 2008, Prevention of Hepatitis B
with the Hepatitis B Vaccine, n engl j med 351;27 www.nejm.org December 30,
2004, Downloaded from www.nejm.org on November 15, 2008 .
Horn Tom and Learned james, 2005, Viral Hepatitis and HIV, AIDS Community
Research Initiative of America (ACRIA): www.acria.
Hargono, Djoko1996, Sekelumit Obat Nabati dan Sistem Imun, Cermin Dunia
Kedokteran No. 108, 1996.
Jagetia GC, Nayak V, Vidyasagar MS, 1998, Evaluation of the antineoplastic activity of
guduchi (Tinospora cordifolia) in cultured HeLa cells. Can Lett. 1998;127:71–82.
Jagetia GC, Rao SK., 2006, Evaluation of the antineoplastic activity of guduchi
(Tinospora cordifolia) in Ehrilch ascites carcinoma bearing mice. Biological and
Pharmaceutical Bulletin. 2006;29:460–6.
Kalikar et al., 2008, Immunomodulatory effect of Tinosporae cordifolia extract in Human
Immuno-deficiency virus Positive Patient, Indian Journal Pharmacology, Juni
2008, Vol 4, issue 3.107-110
57
Kresno, R.J.B., 2001, Imunologi : Diagnosis dan Prosedur Laboratoirium, ed IV,
Fakultas Kedokteran Universitas Indonesia, Jakarta.
Kresno, S.B., 2001, Imunologis : Diagnosis dan Prosedur Laboratorium,Balai Penerbit
FKUI, Jakarta, 5
Kisworo, Heru, 2008, Pengaruh Pemberian Ekstrak Kloroform Buah Mengkudu
(Morindra citrifolia L.) Terhadap Titer Imunoglobulin G (IgG) dan Prolifersi
Limfosit pada mencit yang diinduksi vaksin Hepatitis B, skripsi, 2008.
Karabulut AB et al., 2002, A Comparison of Erythrocyte Superoxide Dismutase and
Catalase Activity in Patients With Hepatitis C Infection, Turk J Med Sci, Vol 32,
hal 313-316
Kapil and Sharma, 1997, Imunopotentiating Compound From Tinospora cordifolia,
Jurnal of Ethnopharmacology, 58 :89-95
Leeson et al.., 1996, Textbook of Histology, diterjemahkan oleh Siswojo et al., Penerbit
Buku Kedokteran EGC, 1996, Jakarta.128
Liang, Jake T, Marcghany, M.D, 2002, National Institute of Diabetesand Digestive and
Kidney Diseases Bethesda, MD 20892 Hepatitis B e Antigen—The Dangerous
Endgame Of Hepatitis B, N Engl J Med, Vol. 347, No. 3·July 18, 2002· 2008 .
Mangkuwijoyo, 2003, Pedoman Workshop Teknologi Dasar Antiibodi Monoklonal,
Laboratorium Ilmu Hayati, UGM, Yogyakarta.
Meghna et al., 2008, Prevention of hepatotoxicity due to anti tuberculosis
treatment: A novel integrative approach, 14 Aguatua 2008, World Journal of
Gastroenterology, , 4753-4762
Manjrekar PN, Jolly CI, Narayanan S, 2000, Comparative studies of the
immunomodulatory activity of Tinospora cordifolia and Tinospora sinensis,
Fitoterapia 71: 254-257.
Metiani R, 2008, Uji Aktivitas Imunostimulator Ekstrak Etanol Jamur Merang
(Volvariella volvaceae) Terhadap Proliferasi limfosit Pada Mencit balb/c Yang
Diinduksi Vaksin Hepatitis B, Skripsi.
Nagarkatti DS et al., 1994, Modulation of Kupffer Cells Activity by Tinospora cordifolia
in Liver Damage, Journal Post Grad Med, 40, hal 62-67
Price and Willson. 2006. Patofisiologi. Ed :6 . Jakarta: EGC.
58
Panchabhai et al., 2008, Protective effect of Tinospora cordifolia, Phyllanthus emblica
and their combination against antitubercular drugs induced hepatic damage: an
experimental study, Jurnal Inter Science, Volume 22, Issue 5 , hal 646 – 650
Published Online: 3 Apr 2008
Ranjith, 2008 , Enhanced Phagocytosis and Antibody Production by Tinospora cordifolia
- A new dimension inImmunomodulation, African Journal of Biotechnology Vol.
7 (2), pp. 081-085, 18 January,
Rantam, 2003, Metode Imunology, Airlangga University Press, Surabaya
Soedarto. 1990. Penyakit-Penyakit Infeksi di Indonesia. Jakarta : Widya Medika.
Sherlock S, 1981, Diseases of the Liver and Billiary System. 6th ed. London: Blackwell
Scient PubI.; 295.
Stead WW, Dutt AK, 1982, Chemotherapy of tuberculosis today. Am. Rev. Res.Dis.;
125: 94–101.
Singh Nisha et al., 2005, Effect of Tinospora cordifolia on Tumor Activity of TumorAssociated macrophagesi-derive Dendritic Cells, Imunopharmacology
Imunotoxicologi 27 (1), hal 1-14
Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC (2003). Chemistry
and medicinal properties of Tinospora cordifolia (guduchi) Indian J. Pharmacol.
35: 83-91. Educational Forum.
Soewignjo Soemohardjo,et al.1983. Tes faal Hati (Dasar-Dasar Teoritik dan Pemakaian
dalam Klinik ). Bandung: Penerbit Alumni.hlm 45-52. Sugiyanto, 1995, Petunjuk
Praktikum Farmakologi, Edisi IV.
Subramoniam A., Pushpangadan P., 1999, Educational Forum Development Of
Phytomedicines For Liver Diseases, Indian Journal of Pharmacology 1999; 31:
166-175
Thatte UM, Kulkarni MR, Dahanukar SA. 1992, Immunotherapeutic modification of
Escherichia coli peritonitis and bacteremia by Tinospora cordifolia. J Postgrad
Med. 1992;38:13.
Thalhah MKA, 2008, Pengaruh Pemberian Ekstrak Kloroform Buah Mengkudu
(Morindra citrifolia L.) Terhadap Titer Imunoglobulin M (IgM) dan Prolifeerasi
Sel Limfosit Pada Rehat Yang Diinduksi Vaksin Hepatitis B.
Wagner, H., 1984, Plant Drug Analysis a Thin Layer Chromatography Atlas, p.164,
Springer-Verlag.
funders: 0
citation:   Rini Prastiwi, Rini  (2009) FEK HEPATOPROTEKTOR DAN IMUNOMODULATOR BATANG BROTOWALI (Tinospora cordifolia Miers) PADA INDUKSI DENGAN VAKSIN HEPATITIS B.  Masters thesis, USB.   
document_url: http://repository.uhamka.ac.id/id/eprint/17168/1/RINI%20PRASTIWI%20TESIS.pdf