eprintid: 13934
rev_number: 8
eprint_status: archive
userid: 2319
dir: disk0/00/01/39/34
datestamp: 2022-08-22 11:58:09
lastmod: 2022-08-22 11:58:09
status_changed: 2022-08-22 11:58:09
type: thesis
metadata_visibility: show
creators_name: Regeswar, Lovina
creators_name: Priyo Wahyudi, Priyo
creators_name: Elly Wardani, Elly
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
title: Penghambatan Biofilm Streptococcus Mutans Dari Hasil Kromatografi Kolom Fraksi Metanol Daun Lempuyang Gajah (Zingiber Zerumbet (L.) Roscoe Ex Sm.)
ispublished: pub
subjects: RS
divisions: 48201
abstract: Streptococcus mutans mensintesis glukan sebagai pengikat dari sukrosa dan mempercepat proses pembentukan biofilm. Daun lempuyang gajah (Zingiber zerumbet (L.) Roscoe ex Sm.) memiliki aktivitas penghambatan biofilm Streptococcus mutans. Penelitian ini bertujuan untuk mengetahui penghambatan biofilm Streptococcus mutans tertinggi dari hasil kromatografi kolom fraksi metanol daun lempuyang gajah. Penelitian ini diawali dengan maserasi bertingkat menggunakan pelarut n-Heksan (non polar), etil asetat (semi polar), dan metanol (polar), kemudian fraksi metanol yang diperoleh dielusi menggunakan kromatografi kolom dengan metode step gradient polarity (SGP). Hasil dari kromatografi kolom, selanjutnya dilakukan pemisahan berdasarkan Rf dengan metode Kromatografi Lapis Tipis (KLT). Hasil kromatografi kolom dengan Rf yang mendekati, kemudian dikelompokkan dan dilakukan uji penghambatan biofilm Streptococcus mutans. Hasil yang didapat menunjukkan bahwa hasil kromatografi kolom fraksi metanol daun lempuyang gajah pada rentang Rf 0,52-0,67 memiliki penghambatan biofilm Streptococcus mutans tertinggi dengan nilai IC50 sebesar 171,48 μg/ml dan potensi relatif 8,04 kali klorheksidin glukonat.

Kata kunci: Daun lempuyang gajah, Hasil kromatografi kolom, Penghambatan biofilm, Streptococcus mutans.
date: 2022-04-15
date_type: completed
full_text_status: restricted
institution: Universitas Muhammadiyah  Dr  Hamka
department: Fakultas Farmasi Dan Sains
thesis_type: bachelor
thesis_name: bphil
referencetext: Agoes G. 2009. Teknologi Bahan Alam Edisi Revisi. Penerbit ITB. Bandung. Hlm. 44. Atlas RM. 2010. Handbook of Microbiological Media 4th Edition. CRC Press Taylor and Francis Group. Boca Raton. Hlm. 227, 249. Baker B, Patricia A, McKernan, Marsik F. 2014. Clinical and Regulatory Development of Antibiofilm Drugs: The Need, The Potential, and The Challenges. Dalam: Raumbaugh P, Ahmad I (Eds.). Antibiofilm Agents From Diagnosis to Treatment and Prevention Volume 8. Springer Sciences and Business Media. New York. Hlm. 475. Bandar H, Hijazi A, Ranmal H, Hachem A, Saad Z, Badran B. 2013. Techniques for The Extraction of Bioactive Compounds From Lebanese Urtica dioica. American Journal of Phytomedicine and clinical Therapeutics. 1(6): 507-513. Baumgartner JC, Siqueira JF, Sedgley CM, Kishen A. 2008. Microbiology of Endodontic Disease. Dalam: Ingle JI, Bakland LK, Baumgartner JC (Eds.). Ingle’s Endodontic. BC Decker Inc. Hamilton. Hlm. 277. Berkovitz BKB, Moxham BJ, Linden RWA, Sloan AJ. 2011. Master Dentistry Volume 3: Oral Biology. Churchill Livingstone. Missouri. Hlm. 96. Bird DL, Robinson DS. 2017. Modern Dental Assisting 12th Edition. Elsevier Saunders. Missouri. Hlm. 169. Black GB. 2008. Microbiology 7th Edition. John Wiley & Sons Inc. Singapore. Hlm. 167, 330-331. Brooks GF, Caroll KC, Butel JS, Morse SA, Mietzner TA. 2010. Jawetz, Melnick, and Adelberg Mikrobiologi Kedokteran Edisi 25. Terjemahan: Nugroho AW, Ramadhani D, Santasa H, Yesdelita N, Nirmala WK. Penerbit Buku Kedokteran EGC. Jakarta. Hlm. 146, 169. Brown. 2012. Benson’s Microbiological Applications: Laboratory Manual in General Microbiology Complete Version 12th Edition. McGraw-Hill. New York. Hlm. 459. Burtis CA, Ashwood ER, Bruns D. 2012. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics 5th. Elsevier Saunders. Missouri. Hlm. 307. Burton GRW, Engelkirk PG. 2011. Microbiology for the health Sciences 10th Edition. Lippincott Co. Philadelphia. Hlm. 325. Cho B, Kim M, Cho J. 2015. Comparison of Streptococcus mutans Biofilm Formation on Dental Materials. International Journal of Clinical Preventive Dentistry. 11(4): 251-260. Costa EM, Silva S, Tavaria FK, Pintado MM. 2013. Study of The Effects of Chitosan upon Streptococcus mutans Adherence and Biofilm Formation. Journal of The Anaerobe Society of The Americans. 20(20Darby ML, Walsh MM. 2010. Dental Hygiene Theory and Practice 3th ed. Saunders Elsevier. Missouri. Hlm. 268, 271, 285, 299. Departemen Kesehatan Republik Indonesia. 2008. Farmakope Herbal Indonesia Edisi I. Departemen Kesehatan Republik Indonesia. Jakarta. Hlm. 169, 172, 174-175. Departemen Kesehatan Republik Indonesia. 2014. Farmakope Indonesia Jilid V. Departemen Kesehatan Republik Indonesia. Jakarta. Hlm. 1588. Enerva LT, Atienza TV, Glifonea ZR, Villamor OB, Villa NA. 2015. Cytotoxicity and Antimikrobial Property of The Leaf Extract of Euphorbia hirta (Tawa-tawa). Journal of Social Science. 3(2015): 167-170. Engelkirk PG, Duben-Engelkirk J. 2015. Burton’s Microbiology for The Health Sciences 10th Edition. Wolters Kluwer Health. Philadelphia. Hlm. 325. Fauzi A. 2009. Aneka Tanaman Obat dan Khasiatnya. Medpress. Yogyakarta. Hlm. 71. Ghasemzadeh A, Jaafar HZE, Ashkani S, Rahmat A, Juraimi AS, Puteh A, Mohamed MTM. 2016. Variation in Secondary Metabolite Production as well as Antioxidant and Bacterial Activities of Zingiber zerumbet L. at Differet Stages of Growth. BMC Complementary and Alternative Medicine. 16: 1-10. Gibbons S. 2006. An Introduction to Planar Chromatography. Dalam: Sarker SD, Latif Z, Gray AI (Eds.). Natural Product Isolation 2nd Edition. Humana Press. New Jersey. Hlm. 87. Gopalsamy B, Farouk AAO, Shah TA, Mohamad T, Sulaiman MR, Perimal EK. 2017. Antiallodynic and Antihyperalgesic Activities of Zerumbone via The Suppression of IL-Iβ, IL-6, and TNF-α in A Mouse Model of Neuropathic Pain. Journal of Pain Research. 10: 2605-2619. Hanani E. 2016. Analisis Fitokimia. Penerbit Buku Kedokteran EGC. Jakarta. Hlm. 10-11, 20, 22, 83, 85-100, 111-113, 149, 183, 233. Harrewijn P, Van-Oosten AM, Piron PGM. 2000. Natural Terpenoids as Messengers: A Multidisciplinary Study of Their Production, Biological Functions and Practical Applications. Springer Science Business Media. Heidelberg. Hlm. 172. Hatrick CD, Eakle WS, Bird WF. 2013. Dental Materials: Clinical Apllications for Dental Assistant and Dental Hygienist 2nd Edition. Elsevier Saunders. Missouri. Hlm. 70. Hean NY, Othman SNAM, Basar N, Jemon K. 2015. Antibiofilm and Antiadhesion Activities of Phaleria macrocarpa Against Oral Streptococcus mutans. Jurnal Teknologi. 77(31): 31-35. Herrera D, Serrano J. 2015. Chemical Oral and Dental Biofilm Control. Dalam: Lindhe J, Lang NP (Eds.). Clinical Periodontology and Implant Dentistry 6th Edition. John Wiley and Sons, Ltd. Oxford. Hlm. 717, 718.
13): 27-31.

Hughes D, Andersson DI. 2004. Antibiotic Development and Resistence. Dalam: Burman LG, Olsson-Liljequist B (Eds.). A Global Perspective on Bacterial Infection, Antibiotic Usage, and The Antibiotic Resistance Problem. Taylor and Francis. London. Hlm. 13. Indrawati NL, Razimin. 2013. Bawang Dayak Si Umbi Ajaib Penakluk Aneka Penyakit. Agro Media Pustaka. Jakarta. Hlm. 85. Kader G, Nikkon F, Rashid MA, Yeasmin T. 2011. Antimicrobial Activities of The Rhizome Extract of Zingiber zerumbet L. Asian Pacific Journal of Biomedicine. 1(5): 409-412. Kementerian Kesehatan Republik Indonesia. 2010. Suplemen I Farmakope Herbal Indonesia. Menteri Kesehatan Republik Indonesia. Jakarta. Hlm. xxi, 98. Kim D, Hwang G, Liu Y, Wang Y, Singh AP, Vorsa N, Koo H. 2015. Cranberry Flavonoids Modulate Cariogenic Properties of Mixed-Species Biofilm Through Exopolysaccharides-Matrix Disruption. Plos One Journal. 10(12): 1-13. Kumpati P. 2008. Bacterial Mutation Assay. Dalam: Gad SC (Ed.). Preclinical Development Handbook Toxicology. John Wiley & Sons Inc. New Jersey. Hlm. 119. Lamont RJ, Jenkinson HF. 2010. Oral Microbiology at Glance. Wiley-Blacwell. Singapore. Hlm. 43. Lee G. 2013. Comparison of Oral Biofilm Formation on Materials of Dental Appliences. International Journal of Clinical Preventive Dentistry. 9(3): 139-144. Liu Y, Xu Y, Song Q, Wang F, Sun L, Liu L, Yang X, Yi J, Bao Y, Ma H, Huang H, Yu C, Huang Y, Wu Y, Li Y. 2017. Anti-biofilm Activities from Bergenia crassifolia Leaves Against Streptococcus mutans. Frontiers in Microbiology. 8(1738): 1-9. Magee P. 2009. Antiseptic Drugs and Disinfectants. Dalam: Aronson JK (Ed.). Side Effects of Drugs Annual: A Worldwide Yearly Survey of New Data and Trends in Adverse Drug Reactions 1st Edition. Elsevier. Oxford. Hlm. 410. Mariotti A, Hefti AF. 2014. Drugs for The Control of Supragingival Plaque. Dalam: Craig CR, Stitzel RE (Eds). Modern Pharmacology with Clinical Applications. Lippincott Williams & Wilkins. Baltimore. Hlm. 499, 502. Marsh PD, Martin MV. 2009. Oral Microbiology 5th Edition. Elsevier Limited. Philadelphia. Hlm. 29, 32, 137-140. Mehdawi IM, Young A. 2014. Antibacterial Composite Restorative Materials for Dental Applications. Dalam: Barnes L, Cooper IR (Eds.). Biomaterials and Medical Device-associated Infections. Woodhead Publishing. Cambridge. Hlm. 200. Miquel S, Lagrafeuille R, Souweine B, Forestier C. Anti-biofilm Activity as A Health Issue. 2016. Dalam: Los Reyes-Gavilan CG, Salazar N (Eds.). Insights Into Microbe-Microbe Interactions in Human Microbial

Ecosystems: Strategies To Be Competitive. Frontiers Media SA. Lausanne Hlm. 47. Mulyani M, Arifin B, Nurdin H. 2013. Uji Antioksidan dan Isolasi Senyawa Metabolit Sekunder dari Daun Srikaya (Annona Squamosa L.). Jurnal Kimia Unand. 2(1): 6-12. Nield-Gehrig JS, Willmann DE. 2012. Foundations of Periodontics for The Dental Hygienist 3rd Edition. Lippincott Williams and Wilkins. Philadelphia. Hlm. 74. Octareza R. 2016. Penapisan Ekstrak Daun Zingiberaceae Sebagai Antibakteri Streptococcus mutans dan Pendegradasi Biofilm pada Gigi. Skripsi. FMIPA IPB, Bogor. Hlm. 14. Odell EW. 2017. Cawson’s Essentials of Oral Pathology and Oral Medicine 9th Edition. Elsevier Saunders. Missouri. Hlm. 55. Perry DA, Beemsterboer PL, Essex G. 2015. Periodontology for The Dental Hygienist 4th Edition. Elsevier Saunders. Missouri. Hlm. 189. Poole CF. 2015. Instrumental Thin Layer Chromatography. Elsevier Inc. Oxford. Hlm. 19. Putri JD. 2017. Aktivitas Antibiofilm Ekstrak Etil Asetat Kulit Buah Manggis (Garcinia mangostana L.) Terhadap Streptococcus mutans. Skripsi. Fakultas Farmasi dan Sains Uhamka, Jakarta. Hlm. 11. Rohman A. 2014. Statistika dan Kemometrika Dasar dalam Analisis Farmasi. Penerbit Pustaka Pelajar. Yogyakarta. Hlm. 121-124. Rubiyanto D. 2017. Metode Kromatografi: Prinsip Dasar, Praktikum, dan Pendekatan Pembelajaran Kromatografi. Penerbit Deepublish. Yogyakarta. Hlm. 2, 8, 22-23, 27-28, 39. Rukmana HR. 2004. Temu-temuan, Apotik Hidup di Pekarangan. Penerbit Kanisius. Yogyakarta. Hlm. 26. Saifudin A. 2014. Senyawa Alam Metabolit Sekunder. Penerbit Deepublish. Yogyakarta. Hlm. 39, 47, 52-55, 62. Sakaue Y, Domon H, Oda M, Takenaka S, Kubo M, Fukuyama Y, Okiji T, Terao Y. 2016. Anti-biofilm and Bactericidal Effects of Magnolia Bark-derived Magnolol and Honokiol on Streptococcus mutans. Journal of Microbiology and Immunology. 60(1): 10-16. Samaranayake LP. 2011. Microbiology for Dentistry 4th Edition. Elsivier Limited. Philadelphia. Hlm. 273, 281. Savitri A. 2016. Tanaman Ajaib! Basmi Penyakit Dengan Tanaman Obat Keluarga. Bibit Publisher. Jakarta. Hlm. 156. Sherma J. 2014. Thin Layer Chromatography. Dalam: Cazes J (Ed.). Ewing’s Analytical Instrumentation Handbook 3rd Edition. CRC Press. Boca Raton. Hlm. 955.

Slobodnikova L, Fialová S, Randecová K, Kováć J, Muĉaji P. 2016. Antibiofilm activity of Plant Polyphenols. Journal of Molecules. 1717(21): 1-15. Soedarto. 2015. Mikrobiologi Kedokteran. Sagung Seto. Jakarta. Hlm. 212. Sumawinata N. 2004. Senarai Istilah Kedokteran Gigi. Penerbit Buku Kedokteran EGC. Jakarta. Hlm. 38. Ulung G. 2014. Sehat Alami dengan Herbal: 250 Tanaman Berkhasiat Obat. Penerbit Gramedia Pustaka Utama. Jakarta. Hlm. 241. Vanmeter KC, Vanmeter WG, Hubert RJ. 2010. Microbiology for The Healthcare Professional. Mosby Elsevier. Missouri. Hlm 130. Wassel MO, Khattab MA. 2017. Antibacterial Activity Against Streptococcus mutans and Inhibition of Bacterial Induced Enamel Demineralization of Propolis, Miswak, and Chitosan Nanoparticles Based Dental Varnishes. Journal of Advanced Research. 8(2017): 387-392. Weiss J, Gulseren I, Kjatannasson G. 2011. Physicochemical Effect of High-Intensity Ultrasonication on Food Proteins and Carbohydrates. Dalam: Zhang HQ, Barbosa-Canovas GV, Balasubramaniam VM, Dunne CP, Farkas DF, Yuan JTC (Eds.). Nonthermal Processing Technologies for Food. Blackwell Publishing Ltd. New Jersey. Hlm, 123. Wilson BA, Salyers AA, Whitt DD, Winkler EM. 2011. Bacterial Pathogenesis: A molecular Approach. ASM Press. Washington D.C. Hlm. 9. Wulandari L. 2011. Kromatografi lapis tipis. PT. Taman Kampus Presindo. Jember. Hlm. 38.
citation:   Regeswar, Lovina dan Priyo Wahyudi, Priyo dan Elly Wardani, Elly  (2022) Penghambatan Biofilm Streptococcus Mutans Dari Hasil Kromatografi Kolom Fraksi Metanol Daun Lempuyang Gajah (Zingiber Zerumbet (L.) Roscoe Ex Sm.).  Bachelor thesis, Universitas Muhammadiyah Dr Hamka.   
document_url: http://repository.uhamka.ac.id/id/eprint/13934/1/FFS_FARMASI_S03-190136_LOVINA%20REGESWARI.pdf