eprintid: 13555
rev_number: 8
eprint_status: archive
userid: 2319
dir: disk0/00/01/35/55
datestamp: 2022-10-09 07:02:09
lastmod: 2022-10-09 07:02:09
status_changed: 2022-10-09 07:02:09
type: thesis
metadata_visibility: show
creators_name: Kusumaningtyas Nova, Aulia
creators_name: Supandi Supandi, Supandi
creators_name: Almawati Situmorang, Almawati
creators_id: supandi@uhamka.ac.id
creators_orcid: 0000-0002-9233-1557
creators_orcid: 0000-0002-9233-1557
title: Optimasi Dan Validasi Metode Analisis Atorvastatin Dalam Plasma In Vitro Secara Kromatografi Cair Kinerja Ultra Tinggi-Tandem Spektrometer Massa (Kckut-Sm)
ispublished: pub
subjects: RS
divisions: 48201
abstract: Atorvastatin termasuk ke dalam golongan obat anti hiperlipidemia yang masuk ke
dalam kategori obat wajib bioekivalensi (BE). Penelitian ini bertujuan untuk
memperoleh kondisi optimum untuk analisis atorvastatin dalam plasma in vitro
menggunakan kromatografi cair kinerja ultra tinggi-tandem spektrometer massa
(KCKUT-SM) dengan detektor Waters Triple Quadropole (TQ) dan melakukan
validasi metode analisis tersebut. Analisis secara kromatografi dilaksanakan
dengan teknik gradient pada kolom fase terbalik kolom BEH C18, fase gerak
asetonitril-asam asetat (variasi 2), dengan laju alir variasi 2, dan dideteksi pada
m/z 559,25>440,38 untuk analit utama dan m/z 260,06> 183,166 untuk baku
dalam. Teknik penyiapan sampel dilakukan dengan cara ekstraksi cair-cair
menggunakan etil asetat. Propranolol digunakan sebagai baku dalam. Metode ini
valid menurut EMEA dalam Guideline on bioanalytical method validation (2011),
dengan persamaan regresi linear y = 0.017x + 0.0048 dan nilai koefisien korelasi
r = 0,9988, kurva linier pada kisaran konsentrasi 1-50 ng/mL, batas terendah
kuantitasi (LLOQ) adalah sebesar 1,0 ng/mL. Hasil uji akurasi atorvastatin intra
hari diperoleh %diff dan antar hari telah memenuhi persyaratan. Validasi pada
penelitian ini dilakukan secara parsial.

Kata kunci: validasi parsial, KCKUT-SM, atorvastatin, plasma in vitro.
date: 2022-03-07
date_type: completed
full_text_status: restricted
institution: Univerisitas Muhammadiyah Dr Hamka
department: Fakultas Farmasi Dan Sains
thesis_type: bachelor
thesis_name: bphil
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citation:   Kusumaningtyas Nova, Aulia dan Supandi Supandi, Supandi dan Almawati Situmorang, Almawati  (2022) Optimasi Dan Validasi Metode Analisis Atorvastatin Dalam Plasma In Vitro Secara Kromatografi Cair Kinerja Ultra Tinggi-Tandem Spektrometer Massa (Kckut-Sm).  Bachelor thesis, Univerisitas Muhammadiyah Dr Hamka.   
document_url: http://repository.uhamka.ac.id/id/eprint/13555/1/FFS_FARMASI_S03-170320_AULIA%20NOVA%20KUSUMANINGTYAS.pdf