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<http://repository.uhamka.ac.id/id/eprint/11591> <http://purl.org/dc/terms/title> "Metode Analisis Thymoquinone\r\nDalam Larutan Dapar Fosfat Ph 7,4 Secara Ultra High Performance Liquid Chromatography (Uhplc)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.uhamka.ac.id/id/eprint/11591> <http://purl.org/ontology/bibo/abstract> "Thymoquinone adalah senyawa aktif yang terdapat dalam jinten hitam (Nigella sativa L) yang memiliki aktivitas sebagai antiinflamasi. Senyawa aktif dari bahan alam memerlukan metode analisis yang valid. Prosedur analisis membutuhkan larutan dapar untuk keberhasilan pengujian. Tujuan penelitian ini untuk validasi metode analisis thymoquinone dalam larutan dapar fosfat pH 7,4 menggunakan Ultra High Performance Liquid Chromatography (UHPLC). Kondisi kromatografi terdiri dari fase gerak metanol - dapar fosfat pH 7,4 (65:35) dan fase diam kolom C18, laju alir 1,0 mL/menit, volume penyuntikan 1 µL pada λ 258 nm. Hasil analisis diperoleh waktu retensi 1,309 menit. Nilai Limit of Detection (LOD) dan Limit of Quantification (LOQ) adalah 5,4235 µg/mL dan 16,4349 µg/mL. Nilai linieritas yang r= 0.9991, %recovery 94,5551%. Uji presisi konsentrasi16,224 ppm, 40,56 ppm, dan 76,05 ppm nilai %RSD sebesar 0,4021%, 0,3299%, dan 0,3748%. Hasil validasi metode analisis telah memenuhi persyaratan Farmakope Edisi V yang ditetapkan sehingga dapat digunakan untuk analisis thymoquinone dalam larutan dapar fosfat pH 7,4.\r\n\r\nKata kunci : Thymoquinone, UHPLC, Validasi Metode."^^<http://www.w3.org/2001/XMLSchema#string> .
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