eprintid: 11090
rev_number: 7
eprint_status: archive
userid: 1993
dir: disk0/00/01/10/90
datestamp: 2021-09-09 09:12:43
lastmod: 2021-09-09 09:12:43
status_changed: 2021-09-09 09:12:43
type: article
metadata_visibility: show
creators_name: Muhamad Arif Budiman, MAB
creators_id: arifbudiman@uhamka.ac.id
creators_orcid: 0000-0002-7202-0735
title: HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA
ispublished: pub
subjects: QD
subjects: R1
divisions: 11201
abstract: Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzymelinked immunosorbent assay) method. Folate binding protein is a protein that can bind folate,therefore it considered can be used as a tool that can replace antibody dependent ELISA method. Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk. Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine's milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide. Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363. Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).
date: 2018-12-29
date_type: published
publisher: Indonesian Society for Biochemistry and Molecular Biology (PBBMI)
official_url: http://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/17
full_text_status: public
publication: Acta Biochimica Indonesiana
volume: 1
number: 2
pagerange: 59-67
refereed: FALSE
issn: 2654-6108
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citation:   Muhamad Arif Budiman, MAB  (2018) HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA.  Acta Biochimica Indonesiana, 1 (2).  pp. 59-67.  ISSN 2654-6108     
document_url: http://repository.uhamka.ac.id/id/eprint/11090/1/271700-human-serum-folate-can-be-measured-using-eabb7741.pdf