<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA"^^ . "Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzymelinked immunosorbent assay) method. Folate binding protein is a protein that can bind folate,therefore it considered can be used as a tool that can replace antibody dependent ELISA method. Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk. Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine's milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide. Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363. Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA)."^^ . "2018-12-29" . . "1" . "2" . . "Indonesian Society for Biochemistry and Molecular Biology (PBBMI)"^^ . . . "Acta Biochimica Indonesiana"^^ . . . "26546108" . . . . . . . "MAB"^^ . "Muhamad Arif Budiman"^^ . "MAB Muhamad Arif Budiman"^^ . . . . . . "HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA (Text)"^^ . . . "271700-human-serum-folate-can-be-measured-using-eabb7741.pdf"^^ . . . "HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #11090 \n\nHUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA\n\n" . "text/html" . . . "QD Chemistry"@en . . . "R Medicine (General)"@en . .