The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.)

Wahyu Hidayati, WahyudanAde Nur Padillah, AdedanMaharadingga, MaharadinggadanNi Putu Ermi Hikmawati, ErmidanRini Prastiwi, RinidanAni Pratiwi, AnidanLady Farahmayuni, LadydanRezza Syahputra, RezzadanMuhammad Fahrul, Fahrul The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.). The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.).

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Abstract

Indonesian people use bay leaves as spices in local culinary and as traditional medicine, particularly to treat diabetes. The problems with the mass production of antidiabetic drugs from bay leaves can be solved by utilizing endophytic fungi as an alternative source for antidiabetic compounds. This study aimed to isolate endophytic fungi from bay leaves and identify their antidiabetic activity through the in vitro inhibition of alpha-amylase. The leaves were processed on potato dextrose agar media, and five isolates were grown in an agar medium. The fermentation used a potato dextrose yeast medium that was left for five days on an orbital shaker at room temperature. The crude was extracted using ethyl acetate solvent. In the in vitro alpha-amylase inhibition test, the antidiabetic assay used the ethyl acetate extract of the endophytic fungi. The inhibition percentage was calculated from the absorbance value read by a microplate reader. All isolates inhibited alpha-amylase activity, but only three of them had high inhibition percentages (14.385%, 12.849%, and 39.246%). As a conclusion, the endophytic fungi isolated from bay leaves are potential as an alternative source for the production of secondary metabolites to cure diabetes.

Item Type: Article
Subjects: Fakultas Agama Islam > R Medicine (General)
Fakultas Agama Islam > RS Pharmacy and materia medica
Divisions: Fakultas Keguruan & Ilmu Pendidikan > School of Medicine
Depositing User: Furqon furqon furqon
Date Deposited: 04 Feb 2020 09:15
Last Modified: 04 Feb 2020 09:15
URI: http://repository.uhamka.ac.id/id/eprint/871

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